Use of modified U1 snRNAs to inhibit HIV-1 replication

Nucleic Acids Research

Volumn 35, Issue 1, 2007, Pages 247-255

  Sajic, R ^a   Lee, K ^a   Asai, K ^a   Sakac, D ^a   Branch, D R ^a   Upton, C ^b   Cochrane, Alan ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF VICTORIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

SMALL NUCLEAR RNA; VIRUS RNA;

ANTIVIRAL ACTIVITY; ARTICLE; GENE CONTROL; GENE EXPRESSION; GENE FUNCTION; GENE TARGETING; GENETIC REGULATION; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SEQUENCE ANALYSIS; VIRUS INHIBITION; WILD TYPE;

ANTI-HIV AGENTS; BASE SEQUENCE; CELL LINE; COMPUTATIONAL BIOLOGY; EXONS; GENE EXPRESSION REGULATION, VIRAL; HIV-1; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RNA, MESSENGER; RNA, SMALL NUCLEAR; RNA, VIRAL; VIRAL PROTEINS; VIRUS REPLICATION;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 33846676217     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkl1022     Document Type: Article

References (42)

1. Schwartz,S., Felber,B.K., Benko,D.M., Fenyo,E.-M. and Pavlakis,G.N. (1990) Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1. J. Virol., 64, 2519-2529.
2. Purcell,D. and Martin,M.A. (1993) Alternative splicing of human immunodeficiency virus type 1 mRNA modulates viral protein expression, replication, and infectivity. J. Virol., 67, 6365-6378.
3. O'Reilly,M.M., McNally,M.T. and Beemon,K.L. (1995) Two strong 5′ (splice sites and competing, suboptimal 3′ splice sites involved in alternative splicing of human immunodeficiency virus type 1 RNA. Virology, 213, 373-385.
4. Staffa,A. and Cochrane,A. (1994) The tat/rev intron of human immunodeficiency virus type 1 is inefficiently spliced because of suboptimal signals in the 3′ splice site. J. Virol., 68, 3071-3079.
5. Si,Z., Amendt,B.A. and Stoltzfus,C.M. (1997) Splicing efficiency of human immunodeficiency virus type 1 tat RNA is determined by both a suboptimal 3′ splice site and a 10 nucleotide exon splicing silencer element located within tat exon 2. Nucleic Acids Res., 25, 861-867.
6. Amendt,B., Si,Z. and Stoltzfus,C.M. (1995) Presence of exon splicing silencers within human immunodeficiency virus type 1 tat exon 2 and tat-rev exon 3: Evidence for inhibition mediated by cellular factors. Mol. Cell. Biol., 15, 4606-4615.
7. Amendt,B.A., Hesslein,D., Chang,L.J. and Stoltzfus,C.M. (1994) Presence of negative and positive cis-acting RNA splicing elements within and flanking the first tat coding exon of human immunodeficiency virus type 1. Mol. Cell. Biol., 14, 3960-3970.
8. Bilodeau,P.S., Domsic,J.K., Mayeda,A., Krainer,A.R. and Stoltzfus,C.M. (2001) RNA splicing at human immunodeficiency virus type 1 3′ splice site A2 is regulated by binding of hnRNP A/B proteins to an exonic splicing silencer element. J. Virol., 75, 8487-8497.
9. Caputi,M., Mayeda,A., Krainer,A. and Zahler,A. (1999) hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO J., 18, 4060-4067.
10. Si,Z.-H., Rauch,D. and Stoltzfus,M. (1998) The exon splicing silencer in the human immunodeficiency virus type 1 tat exon 3 is bipartite and acts early in spliceosome assembly. Mol. Cell. Biol., 18, 5404-5413.
11. Staffa,A. and Cochrane,A. (1995) Identification of positive and negative splicing regulatory elements within the terminal tat-rev exon of human immunodeficiency virus type 1. Mol. Cell. Biol., 15, 4597-4605.
12. McLaren,M., Asai,K. and Cochrane,A. (2004) A novel function for Sam68: enhancement of HIV-1 RNA 3′ end processing. RNA, 10, 1119-1129.
13. Pongoski,J., Asai,K. and Cochrane,A. (2002) Positive and negative modulation of human immunodeficiency virus type 1 rev function by cis and trans regulators of viral RNA splicing. J. Virol., 76, 5108-5120.
14. Zhao,J., Hyman,L. and Moore,C. (1999) Formation of mRNA 3′ ends in eukaryotes: Mechanism, regulation, and interrelationships with other steps in mRNA synthesis. Microbiol. Mol. Biol. Rev., 63, 405-445.
15. Colgan,D.F. and Manley,J.L. (1997) Mechanism and regulation of mRNA polyadenylation. Genes Dev., 11, 2755-2766.
16. Ashe,M.P., Pearson,L.H. and Proudfoot,N.J. (1997) The HIV-1 5′ LTR poly(A) site is inactivated by U1 snRNP interaction with the downstream major splice donor site. EMBO J., 16, 5752-5763.
17. Furth,P.A., Choe,W.T., Rex,J.H., Byrne,J.C. and Baker,C.C. (1994) Sequences homologous to 5′ splice sites are required for the inhibitory activity of papillomavirus late 3′ untranslated regions. Mol. Cell. Biol., 14, 5278-5289.
18. Gunderson,S.I., Polycarpou-Schwarz,M. and Mattaj,I.W. (1998) U1 snRNP inhibits pre-mRNA polyadenylation through a direct interaction between U1 70K and poly(A) polymerase. Mol. Cell, 1, 255-264.
19. Kato,K., Hitomi,Y., Imamura,K. and Esumi,H. (2002) Hyperstable, U1snRNA complementary to the K-ras transcripts induces cell death in pancreatic cancer cells. Br. J. Cancer, 87, 898-904.
20. Vagner,S., Ruegsegger,U., Gunderson,S.I., Keller,W. and Mattaj,I.W. (2000) Position-dependent inhibition of the cleavage step of pre-mRNA 3′-end processing by U1 snRNP. RNA, 6, 178-188.
21. Beckley,S.A., Liu,P., Stover,M.L., Gunderson,S.I., Lichtler,A.C. and Rowe,D.W. (2001) Reduction of target gene expression by a modified U1 snRNA. Mol. Cell. Biol., 21, 2815-2825.
22. Fortes,P., Cuevas,Y., Guan,F., Liu,P., Pentlicky,S., Jung,S.P., Martinez-Chantar,M.L., Prieto,J., Rowe,D. and Gunderson,S.I. (2003) Inhibiting expression of specific genes in mammalian cells with 5′ end-mutated U1 small nuclear RNAs targeted to terminal exons of pre-mRNA. Proc. Natl Acad. Sci. USA, 100, 8264-8269.
23. Liu,P., Gucwa,A., Stover,M.L., Buck,E., Lichtler,A. and Rowe,D. (2002) Analysis of inhibitory action of modified U1 snRNAs on target gene expression discrimination of two RNA targets differing by a 1 bp mismatch. Nucleic Acids Res., 30, 2329-2339.
24. Kriegler,M. (1990) Gene Transfer and Expression: A Laboratory Manual, 1st edn. Stockton Press, NY.
25. Chomczynski,P. and Sacchi,N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
26. Branch,D.R., Valenta,L.J., Yousefi,S., Sakac,D., Singla,R., Bali,M., Sahai,B.M. and Ma,X.Z. (2002) VPAC1 is a cellular neuroendocrine receptor expressed on T cells that actively facilitates productive HIV-1 infection. AIDS, 16, 309-319.
27. Makalowski,W. (2001) The human genome structure and organization. Acta Biochim. Pol., 48, 587-598.
28. Liu,D., Donegan,J., Nuovo,G., Mitra,D. and Laurence,J. (1997) Stable human immunodeficiency virus type 1 (BIV-1) resistance in transformed CD4+ monocytic cells treated with multitargeting HIV-1 antisense sequences incorporated into U1 snRNA. J. Virol., 71, 4079-4085.
29. Ashe,M.P., Furger,A. and Proudfoot,N.J. (2000) Stem-loop 1 of the U1 snRNP plays a critical role in the suppression of HIV-1 polyadenylation. RNA, 6, 170-177.
30. Proudfoot,N.J., Furger,A. and Dye,M.J. (2002) Integrating mRNA processing with transcription. Cell, 108, 501-512.
31. Huang,Y. and Carmichael,G.C. (1996) Role of polyadenylation in nucleocytoplasmic transport of mRNA. Mol. Cell. Biol., 16, 1534-1542.
32. Ford,L.P., Bagga,P.S. and Wilusz,J. (1997) The poly(A) tail inhibits the assembly of a 3′-to-5′ exonuclease in an in vitro RNA stability system. Mol. Cell. Biol., 17, 398-406.
33. Preiss,T. and Hentze,M.W. (1999) From factors to mechanisms: Translation and translational control in eukaryotes. Curr. Opin. Gen. Dev., 9, 515-521.
34. Maniatis,T. and Reed,R. (2002) An extensive network of coupling among gene expression machines. Nature, 416, 499-506.
35. Reed,R. (2003) Coupling transcription, splicing and mRNA export. Curr. Opin. Cell. Biol., 15, 326-331.
36. Vasudevan,S. and Peltz,S.W. (2003) Nuclear mRNA surveillance. Curr. Opin. Cell Biol., 15, 332-337.
37. Tang,H., Kuhen,K.L. and Wong-Staal,F. (1999) Lentivirus replication and regulation. Annu. Rev. Gen., 33, 133-170.
38. Will,C.L. and Luhrmann,R. (2001) Spliceosomal UsnRNP biogenesis, structure and function. Curr. Opin. Cell. Biol., 13, 290-301.
39. Jacque,J.M., Triques,K. and Stevenson,M. (2002) Modulation of HIV-1 replication by RNA interference. Nature, 418, 435-438.
40. Lee,N.S. and Rossi,J.J. (2004) Control of HIV-1 replication by RNA interference. Virus Res., 102, 53-58.
41. Michienzi,A., Castanotto,D., Lee,N., Li,S., Zaia,J.A. and Rossi,J.J. (2003) RNA-mediated inhibition of HIV in a gene therapy setting. Ann. N. Y. Acad. Sci., 1002, 63-71.
42. Boden,D., Pusch,O., Silbermann,R., Lee,F., Tucker,L. and Ramratnam,B. (2004) Enhanced gene silencing of HIV-1 specific siRNA using microRNA designed hairpins. Nucleic Acids Res., 32, 1154-1158.