Perfusion culture improves the maintenance of cultured liver tissue slices

Tissue Engineering

Volumn 13, Issue 1, 2007, Pages 197-205

  Schumacher, Karl ^a   Khong, Yuet Mei ^a,b   Chang, Shi ^c   Ni, Jun ^a,b   Sun, Wanxin ^a   Yu, Hanry ^a,b,c  

^a INSTITUTE OF BIOENGINEERING AND NANOTECHNOLOGY   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; BIOLOGICAL ORGANS; BIOMEDICAL ENGINEERING; BLOOD; METABOLISM; PROTEINS;

BLOOD SUPPLY; EXTRACELLULAR MATRIX (ECM); NON-PARENCHYMAL CELLS; XENOBIOTICS;

CELL CULTURE;

COLLAGEN TYPE 4; HEPATOCYTE NUCLEAR FACTOR 4ALPHA; VIMENTIN;

ANIMAL TISSUE; CELL FUNCTION; CELL INTERACTION; CELL STRUCTURE; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; EXTRACELLULAR MATRIX; LIVER; LIVER PARENCHYMA; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; TISSUE CULTURE; XENOBIOTIC METABOLISM;

ANIMALS; BIOLOGICAL MARKERS; GENE EXPRESSION REGULATION; HEPATOCYTES; LIVER; MALE; PERFUSION; PROTEINS; RATS; RATS, WISTAR; TISSUE CULTURE TECHNIQUES;

EID: 33846570333     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/ten.2006.0046     Document Type: Conference Paper

References (24)

1. Bhatia, S. N., Yarmush, M. L. and Toner, M. Controlling cell interactions by micropatterning in co-cultures: hepatocytes and 3T3 flbroblasts. J Biomed Mater Res 34, 189, 1997.
2. Chia, S. M., Lin, P. C. and Yu, H. TGF-beta1 regulation in hepatocyte-NIH3T3 co-culture is important for the enhanced hepatocyte function in 3D microenvironment. Biotechnol Bioeng 89, 565, 2005.
3. Bedossa, P. and Paradis, V. Liver extracellular matrix in health and disease. J Pathol 200, 504, 2003.
4. Moronvalle-Halley, V., Sacre-Salem, B., Sallez, V., Labbe, G. and Gautier, J. C. Evaluation of cultured, precision-cut rat liver slices as a model to study drug-induced liver apoptosis. Toxicology 207, 203, 2005.
5. Lerche-Langrand, C. and Toutain, H. J. Precision-cut liver slices: characteristics and use for in vitro pharmaco-toxicology. Toxicology 153, 221, 2000.
6. Vickers, A. E. and Fisher, R. L. Organ slices for the evaluation of human drug toxicity. Chem Biol Interact 150, 87, 2004.
7. Martin, H., Bournique, B., Blanchi, B. and Lerche-Langrand, C. High-throughput optimization by statistical designs: example with rat liver slices cryopreservation. Anal Biochem 319, 56, 2003.
8. Olinga, P. et al. Comparison of five incubation systems for rat liver slices using functional and viability parameters. J Pharmacol Toxicol Methods 38, 59, 1997.
9. Minuth, W. W. et al Modulation of cell differentiation in perfusion culture. Exp Nephrol 7, 394, 1999.
10. Schumacher, K., Strehl, R. and Minuth, W. W. Urea restrains aldosterone-induced development of peanut agglutinin-binding on embryonic renal collecting duct epithelia. J Am Soc Nephrol 14, 2758, 2003.
11. Otsu, N. A threshold selection method from gray level histograms. IEEE Trans Syst Man Cybern 9, 62, 1979.
12. Serra, J. Image Analysis and Mathematical Morphology. New York: Academic Press, 1982.
13. Nonaka, H. et al. Development of highly functional long-term culture method of liver slice embedded in agarose gel for bioartificial liver. Cell Transplant 12, 491, 2003.
14. Zeilinger, K. et al. Time course of primary liver cell reorganization in three-dimensional high-density bioreactors for extracorporeal liver support: an immunohistochemical and ultrastructural study. Tissue Eng 10, 1113, 2004.
15. Odom, D. T. et al. Control of pancreas and liver gene expression by HNF transcription factors. Science 303, 1378, 2004.
16. Amenta, P. S. and Harrison, D. Expression and potential role of the extracellular matrix in hepatic ontogenesis: a review. Microsc Res Tech 39, 372, 1997.
17. Martin, H. et al. Induction of cytochrome P450 2B6 and 3A4 expression by phenobarbital and cyclophosphamide in cultured human liver slices. Pharm Res 20, 557, 2003.
18. Hashemi, E., Dobrota, M., Till, C. and Ioannides, C. Structural and functional integrity of precision-cut liver slices in xenobiotic metabolism: a comparison of the dynamic organ and multiwell plate culture procedures. Xenobiotica 29, 11, 1999.
19. McKee, R. L. et al. Perifused precision-cut liver slice system for the study of hormone-regulated hepatic glucose metabolism. J Pharmacol Methods 19, 339, 1988.
20. Kan, P., Miyoshi, H. and Ohshima, N. Perfusion of medium with supplemented growth factors changes metabolic activities and cell morphology of hepatocyte-nonparenchymal cell coculture. Tissue Eng 10, 1297, 2004.
21. Kan, P., Miyoshi, H., Yanagi, K. and Ohshima, N. Effects of shear stress on metabolic function of the co-culture system of hepatocyte/nonparenchymal cells for a bioartificial liver. Asaio J 44, M441, 1998.
22. Koebe, H. G., Wick, M., Cramer, U., Lange, V. and Schildberg, F. W. Collagen gel immobilisation provides a suitable cell matrix for long term human hepatocyte cultures in hybrid reactors. Int J Artif Organs 17, 95, 1994.
23. Tilles, A. W., Baskaran, H., Roy, P., Yarmush, M. L. and Toner, M. Effects of oxygenation and flow on the viability and function of rat hepatocytes cocultured in a microchannel flat-plate bioreactor. Biotechnol Bioeng 73, 379, 2001.
24. Shigematsu, A., Motoji, N., Momose, Y., Iida, A. and Higashi, N. Viability of liver slices exhibiting absorption, metabolism, and elimination of substrates in culture medium. Exp Mol Pathol 69, 119, 2000.