Overexpression of biologically active VEGF121 fusion proteins in Escherichia coli

Journal of Biotechnology

Volumn 128, Issue 3, 2007, Pages 638-647

  Kim, Sehoon ^a   Mohamedali, Khalid A ^a   Cheung, Lawrence H ^a   Rosenblum, Michael G ^a  

^a UNIVERSITY OF TEXAS   (United States)

Author keywords

E. coli AD494 (DE3) pLysS; Fed batch cultivation; Vascular endothelial growth factor (VEGF); VEGF121 rGel

Indexed keywords

CELLS; ESCHERICHIA COLI; GENETIC ENGINEERING; GLYCEROL; GROWTH KINETICS; TUMORS;

CARBON SOURCE; FED-BATCH CULTIVATION; TUMOR ENDOTHELIAL CELLS; VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF);

PROTEINS;

GLYCEROL; HYBRID PROTEIN; POLYHISTIDINE TAG; VASCULOTROPIN 121; VASCULOTROPIN RECEPTOR 2;

ARTICLE; BIOREACTOR; CARBON SOURCE; CELL DENSITY; CULTURE MEDIUM; CYTOTOXICITY; ESCHERICHIA COLI; FED BATCH CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN TARGETING; YEAST;

ANIMALS; ANTINEOPLASTIC AGENTS; BIOREACTORS; CELLS, CULTURED; CLONING, MOLECULAR; ESCHERICHIA COLI; PLANT PROTEINS; PROTEIN ENGINEERING; PROTEIN ISOFORMS; RECOMBINANT FUSION PROTEINS; SWINE; TRANSFECTION; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

ESCHERICHIA COLI;

EID: 33846358984     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.11.027     Document Type: Article

References (28)

1. 121/gelonin chimeric protein. Mol. Pharmacol. 68 6 (2005) 1543-1550
2. Arora N., Masood R., Zheng T., Cai J., Smith D.L., and Gill P.S. Vascular endothelial growth factor chimeric toxin is highly active against endothelial cells. Cancer Res. 59 (1999) 183-188
3. Backer M.V., and Backer J.M. Functionally active VEGF fusion proteins. Protein Express. Purif. 23 1 (2001) 1-7
4. Better M. T-cell-targeted immunofusion proteins from E. coli. Ann. NY Acad. Sci. 782 1 (1996) 544-554
5. Choi S.-J., Park D.-H., and Jung K.-H. Development and optimization of two-stage cyclic fed-batch culture for hG-CSF production using l-arabinose promoter of Escherichia coli. Bioprocess Biosyst. Eng. 24 (2001) 51-58
6. Frings E., Sauer T., and Galinski E.A. Production of hydroxyectoine: high cell-density cultivation and osmotic downshock of Marinococcus strain M52. J. Biotechnol. 43 (1995) 53-61
7. Harrison J.S., Keshavarz-Moore E., Dunnill P., Berry M.J., Fellinger A., and Frenken L. Factors affecting the fermentative production of a lysozyme-binding antibody fragment in Escherichia coli. Biotechnol. Bioeng. 53 6 (1997) 611-622
8. Hoffman B.J., Broadwater J.A., Johnson P., Harper J., Fox B.G., and Kenealy W.R. Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21(DE3): process control yielding high level of metal-incorporated, soluble protein. Protein Express. Purif. 6 (1995) 646-654
9. Hotz H.G., Gill P.S., Masood R., Hotz B., Buhr H.J., Foitzik T., Hines O.J., and Reber H.A. Specific targeting of tumor vasculature by diphtheria toxin-vascular endothelial growth factor fusion protein reduces angiogenesis and growth of pancreatic cancer. J. Gastrointest. Surg. 6 2 (2002) 159-166
10. Jeong K.J., and Lee S.Y. Secretory production of human granulocyte colony stimulating factor in Escherichia coli. Protein Express. Purif. 23 (2001) 311-318
11. Jurado P., Ritz D., Beckwith J., Lorenzo V., and Fernández L.A. Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli. J. Mol. Biol. 320 (2002) 1-10
12. 121/rGel inhibits the growth of orthotopic human bladder carcinoma tumors. Neoplasia 7 10 (2005) 912-920
13. Morera Y., Lamdan H., Bequet M., Ayala M., Rojas G., Muñoz Y., and Gavilondo J.V. Biologically active vascular endothelial growth factor as a bacterial recombinant glutathione S-transferase fusion protein. Biotechnol. Appl. Biochem. 44 (2006) 45-53
14. Plate K.H., Breier G., Weich H.A., and Risau W. Vascular endothelial growth factor is a potential tumor angiogenesis factor in human gliomas in vivo. Nature 359 (1992) 845-848
15. Provoda C.J., Stier E.M., and Lee K.-D. Tumor cell killing enabled by listeriolysin O-liposome-mediated delivery of the protein toxin gelonin. J. Biol. Chem. 278 37 (2003) 35102-35108
16. 121/rGel inhibits pulmonary metastases of MDA-MB-231 breast tumors. Neoplasia 7 5 (2005) 486-496
17. Richard C., Drider D., Elmorjani K., Marion D., and Prévost H. Heterologous expression and purification of active divercin V41, a class IIa bacteriocin encoded by a synthetic gene in Escherichia coli. J. Bacteriol. 186 13 (2004) 4276-4284
18. Roy H., Bhardwaj S., and Ylä-Herttuala S. Biology of vascular endothelial growth factors. FEBS Lett. 580 (2006) 2879-2887
19. Sandee D., Tungpradabkul S., Kurokawa Y., Fukui K., and Takagi M. Combination of Dsb coexpression and an addition of sorbitol markedly enhanced soluble expression of single-chain Fv in Escherichia coli. Biotechnol. Bioeng. 91 4 (2005) 418-424
20. Sandvig K., and Deurs B. Delivery into cells: lessons learned from plant and bacterial toxins. Gene Therapy 12 (2005) 865-872
21. Santala V., and Lamminmäki U. Production of a biotinylated single-chain antibody fragment in the cytoplasm of Escherichia coli. J. Immunol. Meth. 284 (2004) 165-175
22. Scrofani S.D., Fabri L.J., Xu P., Maccarone P., and Nash A.D. Purification and refolding of vascular endothelial growth factor-B. Protein Sci. 9 10 (2000) 2018-2025
23. Shin C.S., Hong M.S., Bae C.S., and Lee J. Enhanced production of human mini-proinsulin in fed-batch cultures at high cell density of Escherichia coli BL21(DE3)[pET-3aT2M2]. Biotechnol. Progr. 13 (1997) 249-257
24. Siemeister G., Schnurr B., Mohrs K., Schächtele C., Marmé D., and Martiny-Baron G. Expression of biologically active isoforms of the tumor angiogenesis factor VEGF in Escherichia coli. Biochem. Biophys. Res. Commun. 222 2 (1996) 249-255
25. Stirpe F., Olsnes S., and Pihl A. Gelonin, a new inhibitor of protein synthesis, nontoxic to intact cells. Isolation, characterization, and preparation of cytotoxic complexes with concanavalin A. J. Biol. Chem. 255 (1980) 6947-6953
26. 121/rGelonin chimeric fusion toxin targeting the neovasculature of solid tumors. Proc. Natl. Acad. Sci. U.S.A. 99 12 (2002) 7866-7871
27. Woestenenk E.A., Hammarström M., Berg S., Härd T., and Berglund H. His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors. J. Struct. Funct. Genom. 5 (2004) 217-229
28. Yuan L.-D., and Hua Z.-C. Expression, purification, and characterization of a biologically active bovine enterokinase catalytic subunit in Escherichia coli. Protein Express. Purif. 25 (2002) 300-304