Ginsenoside Rb1 promotes adipogenesis in 3T3-L1 cells by enhancing PPARγ2 and C/EBPα gene expression

Life Sciences

Volumn 80, Issue 7, 2007, Pages 618-625

  Shang, Wenbin ^a,b   Yang, Ying ^a   Jiang, Boren ^a   Jin, Hua ^a   Zhou, Libin ^a   Liu, Shangquan ^a   Chen, Mingdao ^a  

^a SHANGHAI SECOND MEDICAL UNIVERSITY   (China)

^b NANJING UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

3T3 L1 adipocytes; Adipogenesis; C EBP ; Ginsenosides Rb1; GLUT1; GLUT4; PPAR ; Proliferation

Indexed keywords

CCAAT ENHANCER BINDING PROTEIN ALPHA; GINSENOSIDE RB 1; GLUCOSE TRANSPORTER 1; GLUCOSE TRANSPORTER 4; MESSENGER RNA; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA 2; AZO COMPOUND; CCAAT ENHANCER BINDING PROTEIN BETA; FABP4 PROTEIN, MOUSE; FATTY ACID BINDING PROTEIN; GINSENOSIDE; GLUCOSE; INSULIN; OIL RED O; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; PLANT EXTRACT; TRIACYLGLYCEROL; UNCLASSIFIED DRUG;

ADIPOCYTE; ADIPOGENESIS; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DOSE RESPONSE; GENE; GENE EXPRESSION; GINSENG; GLUCOSE TRANSPORT; LIPID STORAGE; MOUSE; NONHUMAN; UPREGULATION; ANIMAL; CELL PROLIFERATION; CELL STRAIN 3T3; CHEMISTRY; CYTOLOGY; DRUG COMBINATION; DRUG EFFECT; FIBROBLAST; GENE EXPRESSION REGULATION; GENETICS; METABOLISM;

PANAX GINSENG;

3T3 CELLS; ADIPOCYTES; ADIPOGENESIS; ANIMALS; AZO COMPOUNDS; CCAAT-ENHANCER-BINDING PROTEIN-BETA; CELL PROLIFERATION; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG COMBINATIONS; FATTY ACID-BINDING PROTEINS; FIBROBLASTS; GENE EXPRESSION REGULATION; GINSENOSIDES; GLUCOSE; INSULIN; MICE; PANAX; PLANT EXTRACTS; PPAR GAMMA; RNA, MESSENGER; TRIGLYCERIDES;

EID: 33846064201     PISSN: 00243205     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.lfs.2006.10.021     Document Type: Article

References (44)

1. Attele A.S., Wu J.A., and Yuan C.S. Ginseng pharmacology: multiple constituents and multiple actions. Biochemical Pharmacology 58 11 (1999) 1685-1693
2. Attele A.S., Zhou Y.P., Xie J.T., Wu J.A., Zhang L., Dey L., Pugh W., Rue P.A., Polonsky K.S., and Yuan C.S. Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component. Diabetes 51 6 (2002) 1851-1858
3. Bays H., Mandarino L., and DeFronzo R.A. Role of the adipocyte, free fatty acids, and ectopic fat in pathogenesis of type 2 diabetes mellitus: peroxisomal proliferator-activated receptor agonists provide a rational therapeutic approach. The Journal of Clinical Endocrinology and Metabolism 89 2 (2004) 463-478
4. Cho J., Park W., Lee S., Ahn W., and Lee Y. Ginsenoside-Rb1 from Panax ginseng C.A. Meyer activates estrogen receptor-{alpha} and -{beta}, independent of ligand binding. The Journal of Clinical Endocrinology and Metabolism 89 7 (2004) 3510-3515
5. Chung S.H., Choi C.G., and Park S.H. Comparisons between white ginseng radix and rootlet for antidiabetic activity and mechanism in KKAy mice. Archives of Pharmacal Research 24 3 (2001) 214-218
6. Dey L., Xie J.T., Wang A., Wu J., Maleckar S.A., and Yuan C.S. Anti-hyperglycemic effects of ginseng: comparison between root and berry. Phytomedicine 10 6-7 (2003) 600-605
7. Ferre P. The biology of peroxisome proliferator-activated receptors: relationship with lipid metabolism and insulin sensitivity. Diabetes 53 Suppl 1 (2004) S43-S50
8. Fruhbeck G., Gomez-Ambrosi J., Muruzabal F.J., and Burrell M.A. The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation. American Journal of Physiology: Endocrinology and Metabolism 280 6 (2001) E827-E847
9. Gillis C.N. Panax ginseng pharmacology: a nitric oxide link?. Biochemical Pharmacology 54 1 (1997) 1-8
10. Gregoire F.M., Smas C.M., and Sul H.S. Understanding adipocyte differentiation. Physiological Reviews 78 3 (1998) 783-809
11. Han L.K., Zheng Y.N., Yoshikawa M., Okuda H., and Kimura Y. Anti-obesity effects of chikusetsusaponins isolated from Panax japonicus rhizomes. BMC Complementary and Alternative Medicine 5 1 (2005) 9
12. Han K.L., Jung M.H., Sohn J.H., and Hwang J.K. Ginsenoside 20(S)-protopanaxatriol (PPT) activates peroxisome proliferator-activated receptor gamma (PPARgamma) in 3T3-L1 adipocytes. Biological & Pharmaceutical Bulletin 29 1 (2006) 110-113
13. Harmon A.W., and Harp J.B. Differential effects of flavonoids on 3T3-L1 adipogenesis and lipolysis. American Journal of Physiology. Cell Physiology 280 4 (2001) C807-C813
14. Kim J.H., Hahm D.H., Yang D.C., Kim J.H., Lee H.J., and Shim I. Effect of crude saponin of Korean red ginseng on high-fat diet-induced obesity in the rat. Journal of Pharmacological Sciences 97 1 (2005) 124-131
15. Lehmann J.M., Moore L.B., Smith-Oliver T.A., Wilkison W.O., Willson T.M., and Kliewer S.A. An antidiabetic thiazolidinedione is a high affinity ligand for peroxisome proliferator-activated receptor gamma (PPAR gamma). The Journal of Biological Chemistry 270 22 (1995) 12953-12956
16. Lehrke M., and Lazar M.A. The many faces of PPAR gamma. Cell 123 6 (2005) 993-999
17. Lim W., Mudge K.W., and Vermeylen F. Effects of population, age, and cultivation methods on ginsenoside content of wild American ginseng (Panax quinquefolium). Journal of Agricultural and Food Chemistry 53 22 (2005) 8498-8505
18. Liu X., Kim J.K., Li Y., Li J., Liu F., and Chen X. Tannic acid stimulates glucose transport and inhibits adipocyte differentiation in 3T3-L1 cells. The Journal of Nutrition 135 2 (2005) 165-171
19. Nawrocki A.R., and Scherer P.E. Keynote review: the adipocyte as a drug discovery target. Drug Discovery Today 10 18 (2005) 1219-1230
20. Park M.Y., Lee K.S., and Sung M.K. Effects of dietary mulberry, Korean red ginseng, and banaba on glucose homeostasis in relation to PPAR-alpha, PPAR-gamma, and LPL mRNA expressions. Life Sciences 77 26 (2005) 3344-3354
21. Ramirez-Zacarias J.L., Castro-Munozledo F., and Kuri-Harcuch W. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97 6 (1992) 493-497
22. Ren D., Collingwood T.N., Rebar E.J., Wolffe A.P., and Camp H.S. PPARgamma knockdown by engineered transcription factors: exogenous PPARgamma2 but not PPARgamma1 reactivates adipogenesis. Genes and Development 16 1 (2002) 27-32
23. Rosen E.D. The transcriptional basis of adipocyte development. Prostaglandins, Leukotrienes, and Essential Fatty Acids 73 1 (2005) 31-34
24. Rosen E.D., and Spiegelman B.M. Molecular regulation of adipogenesis. Annual Review of Cell and Developmental Biology 16 (2000) 145-171
25. Sekiya K., and Okuda H. Enhancement of adipose differentiation of mouse 3T3-L1 fibroblasts by ginsenosides. Phytotherapy Research 1 2 (1987) 58-60
26. Sengupta S., Toh S.A., Sellers L.A., Skepper J.N., Koolwijk P., Leung H.W., Yeung H.W., Wong R.N., Sasisekharan R., and Fan T.P. Modulating angiogenesis: the yin and the yang in ginseng. Circulation 110 10 (2004) 1219-1225
27. Shepherd P.R., Gnudi L., Tozzo E., Yang H., Leach F., and Kahn B.B. Adipose cell hyperplasia and enhanced glucose disposal in transgenic mice overexpressing GLUT4 selectively in adipose tissue. The Journal of Biological Chemistry 268 30 (1993) 22243-22246
28. Sotaniemi E.A., Haapakoski E., and Rautio A. Ginseng therapy in non-insulin-dependent diabetic patients. Diabetes Care 18 10 (1995) 1373-1375
29. Spiegelman B.M. PPAR-gamma: adipogenic regulator and thiazolidinedione receptor. Diabetes 47 4 (1998) 507-514
30. Tafuri S.R. Troglitazone enhances differentiation, basal glucose uptake, and Glut1 protein levels in 3T3-L1 adipocytes. Endocrinology 137 11 (1996) 4706-4712
31. Twentyman P.R., and Luscombe M. A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. British Journal of Cancer 56 3 (1987) 279-285
32. Vuksan V., Sievenpiper J.L., Koo V.Y., Francis T., Beljan-Zdravkovic U., Xu Z., and Vidgen E. American ginseng (Panax quinquefolius L) reduces postprandial glycemia in nondiabetic subjects and subjects with type 2 diabetes mellitus. Archives of Internal Medicine 160 7 (2000) 1009-1013
33. Wang H., Reaves L.A., and Edens N.K. Ginseng extract inhibits lipolysis in rat adipocytes in vitro by activating phosphodiesterase 4. The Journal of Nutrition 136 2 (2006) 337-342
34. Washida D., and Kitanaka S. Determination of polyacetylenes and ginsenosides in Panax species using high performance liquid chromatography. Chemical and Pharmaceutical Bulletin (Tokyo) 51 11 (2003) 1314-1317
35. Weiland M., Schurmann A., Schmidt W.E., and Joost H.G. Development of the hormone-sensitive glucose transport activity in differentiating 3T3-L1 murine fibroblasts. Role of the two transporter species and their subcellular localization. The Biochemical Journal 270 2 (1990) 331-336
36. Wood I.S., and Trayhurn P. Glucose transporters (GLUT and SGLT): expanded families of sugar transport proteins. The British Journal of Nutrition 89 1 (2003) 3-9
37. Xie J.T., Aung H.H., Wu J.A., Attel A.S., and Yuan C.S. Effects of American ginseng berry extract on blood glucose levels in ob/ob mice. The American Journal of Chinese Medicine 30 2-3 (2002) 187-194
38. Xie J.T., Zhou Y.P., Dey L., Attele A.S., Wu J.A., Gu M., Polonsky K.S., and Yuan C.S. Ginseng berry reduces blood glucose and body weight in db/db mice. Phytomedicine 9 3 (2002) 254-258
39. Xie J.T., Mehendale S.R., Li X., Quigg R., Wang X., Wang C.Z., Wu J.A., Aung H.H., P, A.R., Bell G.I., and Yuan C.S. Anti-diabetic effect of ginsenoside Re in ob/ob mice. Biochimica et Biophysica Acta 1740 3 (2005) 319-325
40. Xie J.T., Wang C.Z., Wang A.B., Wu J., Basila D., and Yuan C.S. Antihyperglycemic effects of total ginsenosides from leaves and stem of Panax ginseng. Acta Pharmacologica Sinica 26 9 (2005) 1104-1110
41. Yang J., Clark A.E., Kozka I.J., Cushman S.W., and Holman G.D. Development of an intracellular pool of glucose transporters in 3T3-L1 cells. The Journal of Biological Chemistry 267 15 (1992) 10393-10399
42. Yang X., Jansson P.A., Nagaev I., Jack M.M., Carvalho E., Sunnerhagen K.S., Cam M.C., Cushman S.W., and Smith U. Evidence of impaired adipogenesis in insulin resistance. Biochemical and Biophysical Research Communications 317 4 (2004) 1045-1051
43. Yun S.N., Moon S.J., Ko S.K., Im B.O., and Chung S.H. Wild ginseng prevents the onset of high-fat diet induced hyperglycemia and obesity in ICR mice. Archives of Pharmacal Research 27 7 (2004) 790-796
44. Zisman A., Peroni O.D., Abel E.D., Michael M.D., Mauvais-Jarvis F., Lowell B.B., Wojtaszewski J.F., Hirshman M.F., Virkamaki A., Goodyear L.J., Kahn C.R., and Kahn B.B. Targeted disruption of the glucose transporter 4 selectively in muscle causes insulin resistance and glucose intolerance. Nature Medicine 6 8 (2000) 924-928