PPARγ knockdown by engineered transcription factors: Exogenous PPARγ2 but not PPARγ1 reactivates adipogenesis

Genes and Development

Volumn 16, Issue 1, 2002, Pages 27-32

  Ren, Delin ^a   Collingwood, Trevor N ^b   Rebar, Edward J ^b   Wolffe, Alan P ^b   Camp, Heidi S ^a  

^a PFIZER INC   (United States)

^b SANGAMO BIOSCIENCES INC   (United States)

Author keywords

Adipogenesis; PPAR 1; PPAR 2; Zinc finger protein

Indexed keywords

ISOPROTEIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; TRANSCRIPTION FACTOR; ZINC FINGER PROTEIN; CELL RECEPTOR;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; BODY MASS; CELL CULTURE; CONTROLLED STUDY; DNA BINDING; GENE EXPRESSION REGULATION; GENE OVEREXPRESSION; GENE STRUCTURE; KNOCKOUT GENE; NONHUMAN; OBESITY; PRIORITY JOURNAL; PROMOTER REGION; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION; TRANSGENIC ANIMAL; ADIPOCYTE; ANIMAL; CELL DIFFERENTIATION; CELL STRAIN 3T3; CYTOLOGY; GENETICS; MOUSE; PHYSIOLOGY; RNA SPLICING;

ANIMALIA;

3T3 CELLS; ADIPOCYTES; ANIMALS; CELL DIFFERENTIATION; GENE EXPRESSION REGULATION; MICE; PROTEIN ISOFORMS; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RNA SPLICING; TRANSCRIPTION FACTORS; ZINC FINGERS;

EID: 0036007025     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.953802     Document Type: Article

References (30)

1. The common PPARγ Pro12Ala polymorphism is associated with decreased risk of type 2 diabetes
2. PPARγ is required for placental, cardiac, and adipose tissue development
3. Regulation of the MDR1 gene by transcriptional repressors selected using peptide combinatorial libraries
4. Association of the Pro12Ala variant in the peroxisome proliferator-activated receptor-γ2 gene with obesity in two Caucasian populations
5. Positive and negative regulation of endogenous genes by designed transcription factors
6. PPARγ activators down-regulate the expression of PPARγ in 3T3-L1 adipocytes
7. A novel potent antagonist of peroxisome proliferator-activated receptor gamma blocks adipocyte differentiation but does not revert the phenotype of terminally differentiated adipocytes
8. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells
9. Selection of DNA binding sites for zinc fingers using rationally randomized DNA reveals coded interactions
10. A Pro12Ala substitution in PPARγ2 associated with decreased receptor activity, lower body mass index and improved insulin sensitivity
11. Redesigning the DNA-binding specificity of a zinc finger protein: A data base-guided approach
12. The organization, promoter analysis, and expression of the human PPARγ gene
13. A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
14. A role for C/EBPα in regulating peroxisome proliferator-activated receptor gamma activity during adipogenesis in 3T3-L1 preadipocytes
15. 1 cyclin-dependent kinases
16. In vitro selection of zinc fingers with altered DNA-binding specificity
17. Differential expression and activation of a family of murine peroxisome proliferator-activated receptors
18. PPARγ mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance
19. Regulation of an endogenous locus using a panel of designed zinc finger proteins targeted to accessible chromatin regions. Activation of vascular endothelial growth factor A
20. Kruppel-associated boxes are potent transcriptional repression domains
21. Role of PPARγ in regulating a cascade expression of cyclin-dependent kinase inhibitors, p18(INK4c) and p21(Waf1/Cip1), during adipogenesis
22. Zinc finger phage: Affinity selection of fingers with new DNA-binding specificities
23. PPARγ is required for the differentiation of adipose tissue in vivo and in vitro
24. mPPARγ2: Tissue-specific regulator of an adipocyte enhancer
25. Stimulation of adipogenesis in fibroblasts by PPARγ2, a lipid-activated transcription factor
26. 1 cyclin-Cdk protein kinase activity, is related to p21
27. Ligand-independent activation domain in the N terminus of peroxisome proliferator-activated receptor gamma (PPARγ). Differential activity of PPARγ-1 and -2 isoforms and influence of insulin
28. PPARγ induces the insulin-dependent glucose transporter GLUT4 in the absence of C/EBPα during the conversion of 3T3 fibroblasts into adipocytes
29. Synthetic zinc finger transcription factor action at an endogenous chromosomal site. Activation of the human erythropoietin gene
30. Structural organization of mouse peroxisome proliferator-activated receptor gamma (mPPARγ) gene: Alternative promoter use and different splicing yield two mPPARγ isoforms