메뉴 건너뛰기




Volumn 63, Issue 1, 2007, Pages 101-112

Effects of fixation and storage on flow cytometric analysis of marine bacteria

Author keywords

Fixation; Flow cytometry; HDNA bacteria; LDNA bacteria; Marine bacteria; Storage

Indexed keywords

BACTERIUM; FIXATION; FLOW CYTOMETRY; MARINE ENVIRONMENT; MICROBIAL COMMUNITY;

EID: 33846022335     PISSN: 09168370     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10872-007-0008-7     Document Type: Article
Times cited : (35)

References (43)
  • 1
    • 0018216331 scopus 로고
    • The effect of chemical fixatives on cell walls of Bacillus subtilis
    • Beveridge, T. J., F. M. R. Williams and J. J. Koval (1978): The effect of chemical fixatives on cell walls of Bacillus subtilis. Can. J. Microbiol., 24, 1439-1451.
    • (1978) Can. J. Microbiol. , vol.24 , pp. 1439-1451
    • Beveridge, T.J.1    Williams, F.M.R.2    Koval, J.J.3
  • 2
    • 0021346973 scopus 로고
    • The current status of fixation for electron microscopy: A review
    • Bullock, G. R. (1984): The current status of fixation for electron microscopy: A review. J. Microsc., 133, 1-15.
    • (1984) J. Microsc. , vol.133 , pp. 1-15
    • Bullock, G.R.1
  • 3
    • 0035317027 scopus 로고    scopus 로고
    • Determination of DNA content of aquatic bacteria by flow cytometry
    • Button, D. K. and B. R. Robertson (2001): Determination of DNA content of aquatic bacteria by flow cytometry. Appl. Environ. Microbiol., 67, 1636-1645.
    • (2001) Appl. Environ. Microbiol. , vol.67 , pp. 1636-1645
    • Button, D.K.1    Robertson, B.R.2
  • 4
    • 0035839320 scopus 로고    scopus 로고
    • Double-stranded DNA measurement in lakes with the fluorescent strain PicoGreen and the application to bacterial bioassays
    • Cotner, J. B., M. L. Ogdahl and B. A. Biddanda (2001): Double-stranded DNA measurement in lakes with the fluorescent strain PicoGreen and the application to bacterial bioassays. Aquat. Microb. Ecol., 25, 65-74.
    • (2001) Aquat. Microb. Ecol. , vol.25 , pp. 65-74
    • Cotner, J.B.1    Ogdahl, M.L.2    Biddanda, B.A.3
  • 5
    • 0029832083 scopus 로고    scopus 로고
    • Flow cytometry and cell sorting of heterogeneous microbial populations: The importance of single-cell analyses
    • Davey, H. M. and D. B. Kell (1996): Flow cytometry and cell sorting of heterogeneous microbial populations: The importance of single-cell analyses. Microbiol. Rev., 60, 641-696.
    • (1996) Microbiol. Rev. , vol.60 , pp. 641-696
    • Davey, H.M.1    Kell, D.B.2
  • 6
    • 0032192340 scopus 로고    scopus 로고
    • Bacterial loss and degradation of bacterial membrane in preserved seawater samples
    • Decamp, O. and N. Rajendran (1998): Bacterial loss and degradation of bacterial membrane in preserved seawater samples. Mar. Pollut. Bull., 36, 856-859.
    • (1998) Mar. Pollut. Bull. , vol.36 , pp. 856-859
    • Decamp, O.1    Rajendran, N.2
  • 7
    • 0029789243 scopus 로고    scopus 로고
    • Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13
    • del Giorgio, P. A., D. F. Bird, Y. T. Prairie and D. Planas (1996): Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13. Limnol. Oceanogr., 41, 783-789.
    • (1996) Limnol. Oceanogr. , vol.41 , pp. 783-789
    • del Giorgio, P.A.1    Bird, D.F.2    Prairie, Y.T.3    Planas, D.4
  • 8
    • 84985088099 scopus 로고
    • An assessment of methods for measuring volumes of planktonic bacteria, with particular reference to television image analysis
    • Fry, J. C. and A. R. Davies (1985): An assessment of methods for measuring volumes of planktonic bacteria, with particular reference to television image analysis. J. Appl. Bacteriol., 58, 105-112.
    • (1985) J. Appl. Bacteriol. , vol.58 , pp. 105-112
    • Fry, J.C.1    Davies, A.R.2
  • 9
    • 0033865159 scopus 로고    scopus 로고
    • Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities
    • Gasol, J. M. and P. A. del Giorgio (2000): Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. Sci. Mar., 64, 197-224.
    • (2000) Sci. Mar. , vol.64 , pp. 197-224
    • Gasol, J.M.1    del Giorgio, P.A.2
  • 10
    • 0000253139 scopus 로고    scopus 로고
    • Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry
    • Gasol, J. M. and X. A. Moran (1999): Effects of filtration on bacterial activity and picoplankton community structure as assessed by flow cytometry. Aquat. Microb. Ecol., 16, 251-264.
    • (1999) Aquat. Microb. Ecol. , vol.16 , pp. 251-264
    • Gasol, J.M.1    Moran, X.A.2
  • 11
    • 0032829065 scopus 로고    scopus 로고
    • Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria
    • Gasol, J. M., U. L. Zweifel, F. Peters, J. A. Fuhrman and A. Hagstrom (1999): Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria. Appl. Environ. Microbiol., 65, 4475-4483.
    • (1999) Appl. Environ. Microbiol. , vol.65 , pp. 4475-4483
    • Gasol, J.M.1    Zweifel, U.L.2    Peters, F.3    Fuhrman, J.A.4    Hagstrom, A.5
  • 12
    • 0036161361 scopus 로고    scopus 로고
    • A transplant experiment to identify the factors controlling bacterial abundance, activity, production, and community composition in a eutrophic canyon-shaped reservoir
    • Gasol, J. M., M. Comerma, J. C. Garcia, J. Armengol, E. O. Casamayor, P. Kojecka and K. Simek (2002): A transplant experiment to identify the factors controlling bacterial abundance, activity, production, and community composition in a eutrophic canyon-shaped reservoir. Limnol. Oceanogr., 47, 62-77.
    • (2002) Limnol. Oceanogr. , vol.47 , pp. 62-77
    • Gasol, J.M.1    Comerma, M.2    Garcia, J.C.3    Armengol, J.4    Casamayor, E.O.5    Kojecka, P.6    Simek, K.7
  • 13
    • 0036122384 scopus 로고    scopus 로고
    • Involvement of RNA and DNA in the staining of Escherichia coli by SYTO 13
    • Guindulain, T. and J. Vives-Rego (2002): Involvement of RNA and DNA in the staining of Escherichia coli by SYTO 13. Lett. Appl. Microbiol., 34, 182-188.
    • (2002) Lett. Appl. Microbiol. , vol.34 , pp. 182-188
    • Guindulain, T.1    Vives-Rego, J.2
  • 14
    • 0030661565 scopus 로고    scopus 로고
    • Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry
    • Guindulain, T., J. Comas and J. Vies-Rego (1997): Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry. Appl. Environ. Microbiol., 63, 4608-4611.
    • (1997) Appl. Environ. Microbiol. , vol.63 , pp. 4608-4611
    • Guindulain, T.1    Comas, J.2    Vies-Rego, J.3
  • 15
    • 0029832917 scopus 로고    scopus 로고
    • Factors influencing the loss of bacteria in preserved seawater samples
    • Gundersen, K., G. Bratbak and M. Heldal (1996): Factors influencing the loss of bacteria in preserved seawater samples. Mar. Ecol. Prog. Ser., 137, 305-310.
    • (1996) Mar. Ecol. Prog. Ser. , vol.137 , pp. 305-310
    • Gundersen, K.1    Bratbak, G.2    Heldal, M.3
  • 16
    • 0010301218 scopus 로고
    • The reaction of formaldehyde with polynucleotides
    • Haselkorn, R. and P. Doty (1961): The reaction of formaldehyde with polynucleotides. J. Biol. Chem., 236, 2738-2745.
    • (1961) J. Biol. Chem. , vol.236 , pp. 2738-2745
    • Haselkorn, R.1    Doty, P.2
  • 17
    • 0029835177 scopus 로고    scopus 로고
    • Metabolic activity of bacterioplankton communities assessed by flow cytometry and single carbon substrate utilization
    • Jellett, J. F., W. K. W. Li, P. M. Dickie, A. Boraie and P. E. Kepkay (1996): Metabolic activity of bacterioplankton communities assessed by flow cytometry and single carbon substrate utilization. Mar. Ecol. Prog. Ser., 136, 213-225.
    • (1996) Mar. Ecol. Prog. Ser. , vol.136 , pp. 213-225
    • Jellett, J.F.1    Li, W.K.W.2    Dickie, P.M.3    Boraie, A.4    Kepkay, P.E.5
  • 18
    • 0035807129 scopus 로고    scopus 로고
    • Morphology and DNA content of bacterioplankton in the northern Gulf of Mexico: Analysis by epifluorescence microscopy and flow cytometry
    • Jochem, F. J. (2001): Morphology and DNA content of bacterioplankton in the northern Gulf of Mexico: Analysis by epifluorescence microscopy and flow cytometry. Cytometry, 25, 179-194.
    • (2001) Cytometry , vol.25 , pp. 179-194
    • Jochem, F.J.1
  • 19
    • 0028117074 scopus 로고
    • Use of fluorochromes for direct enumeration of total bacteria in environmental samples: Past and present
    • Kepner, R. L. and J. R. Pratt (1994): Use of fluorochromes for direct enumeration of total bacteria in environmental samples: Past and present. Microbiol. Rev., 58, 603-615.
    • (1994) Microbiol. Rev. , vol.58 , pp. 603-615
    • Kepner, R.L.1    Pratt, J.R.2
  • 20
    • 0018423107 scopus 로고
    • A tentative direct microscopic method for counting living marine bacteria
    • Kogure, K., U. Simidu and N. Taga (1979): A tentative direct microscopic method for counting living marine bacteria. Can. J. Microbiol., 25, 415-420.
    • (1979) Can. J. Microbiol. , vol.25 , pp. 415-420
    • Kogure, K.1    Simidu, U.2    Taga, N.3
  • 21
    • 0031596935 scopus 로고    scopus 로고
    • Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems
    • Lebaron, P., N. Parthuisot and P. Catala (1998): Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems. Appl. Environ. Microbiol., 64, 1725-1730.
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 1725-1730
    • Lebaron, P.1    Parthuisot, N.2    Catala, P.3
  • 22
    • 0035318548 scopus 로고    scopus 로고
    • Does the high nucleic acid content of individual bacterial cells allow us to discriminate between active cells and inactive cells in aquatic systems?
    • Lebaron, P., P. Servais and H. Agogue (2001): Does the high nucleic acid content of individual bacterial cells allow us to discriminate between active cells and inactive cells in aquatic systems? Appl. Environ. Microbiol., 67, 1775-1782.
    • (2001) Appl. Environ. Microbiol. , vol.67 , pp. 1775-1782
    • Lebaron, P.1    Servais, P.2    Agogue, H.3
  • 23
    • 0028995826 scopus 로고
    • DNA distributions in planktonic bacteria stained with TOTO or TO-PRO
    • Li, W. K. W., J. F. Jellett and P. M. Dickie (1995): DNA distributions in planktonic bacteria stained with TOTO or TO-PRO. Limnol. Oceanogr., 40, 1485-1495.
    • (1995) Limnol. Oceanogr. , vol.40 , pp. 1485-1495
    • Li, W.K.W.1    Jellett, J.F.2    Dickie, P.M.3
  • 24
    • 0029883988 scopus 로고    scopus 로고
    • Application of novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes
    • Marie, D., D. Vaulot and F. Partensky (1996): Application of novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes. Appl. Environ. Microbiol., 62, 1649-1655.
    • (1996) Appl. Environ. Microbiol. , vol.62 , pp. 1649-1655
    • Marie, D.1    Vaulot, D.2    Partensky, F.3
  • 25
    • 0030620443 scopus 로고    scopus 로고
    • Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I
    • Marie, D., F. Partensky, S. Jacquet and D. Vaulot (1997): Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I. Appl. Environ. Microbiol., 63, 186-193.
    • (1997) Appl. Environ. Microbiol. , vol.63 , pp. 186-193
    • Marie, D.1    Partensky, F.2    Jacquet, S.3    Vaulot, D.4
  • 26
    • 0003244274 scopus 로고    scopus 로고
    • Enumeration of phytoplankton, bacteria and viruses in marine samples
    • In ed. by J. P. Robinson, John Wiley & Sons, Inc., New York
    • Marie, D., F. Partensky, D. Vaulot and C. Brussaard (1999): Enumeration of phytoplankton, bacteria and viruses in marine samples. p. 11.11.1-11.11.15. In Current Protocols in Cytometry, ed. by J. P. Robinson, John Wiley & Sons, Inc., New York.
    • (1999) Current Protocols in Cytometry
    • Marie, D.1    Partensky, F.2    Vaulot, D.3    Brussaard, C.4
  • 28
    • 0001679973 scopus 로고    scopus 로고
    • Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria
    • Noble, R. T. and J. A. Fuhrman (1998): Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria. Aquat. Microb. Ecol., 14, 113-118.
    • (1998) Aquat. Microb. Ecol. , vol.14 , pp. 113-118
    • Noble, R.T.1    Fuhrman, J.A.2
  • 29
    • 22044441984 scopus 로고    scopus 로고
    • Simultaneous measurement of metabolic activity and membrane integrity in marine bacterioplankton determined by confocal laser-scanning microscopy
    • Pirker, H., C. Pausz, K. E. Stoderegger and G. J. Herndl (2005): Simultaneous measurement of metabolic activity and membrane integrity in marine bacterioplankton determined by confocal laser-scanning microscopy. Aquat. Microb. Ecol., 39, 225-233.
    • (2005) Aquat. Microb. Ecol. , vol.39 , pp. 225-233
    • Pirker, H.1    Pausz, C.2    Stoderegger, K.E.3    Herndl, G.J.4
  • 30
    • 0017087978 scopus 로고
    • Histone redistribution and conformational effect on chromatin induced by formaldehyde
    • Polacow, I., L. Cabasso and H. J. Li (1976): Histone redistribution and conformational effect on chromatin induced by formaldehyde. Biochemistry, 15, 4559-4565.
    • (1976) Biochemistry , vol.15 , pp. 4559-4565
    • Polacow, I.1    Cabasso, L.2    Li, H.J.3
  • 31
    • 84986870589 scopus 로고
    • The use of DAPI for identifying and counting aquatic microflora
    • Porter, K. G. and Y. S. Feig (1980): The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr., 25, 943-948.
    • (1980) Limnol. Oceanogr. , vol.25 , pp. 943-948
    • Porter, K.G.1    Feig, Y.S.2
  • 33
    • 33747595639 scopus 로고    scopus 로고
    • Comparison of SYBR Green I and SYBR Gold stains for enumerating bacteria and viruses by epifluorescence microscopy
    • Shibata, A., Y. Goto, H. Saito, T. Kikuchi, T. Toda and S. Taguchi (2006): Comparison of SYBR Green I and SYBR Gold stains for enumerating bacteria and viruses by epifluorescence microscopy. Aquat. Microb. Ecol., 43, 223-231.
    • (2006) Aquat. Microb. Ecol. , vol.43 , pp. 223-231
    • Shibata, A.1    Goto, Y.2    Saito, H.3    Kikuchi, T.4    Toda, T.5    Taguchi, S.6
  • 34
    • 0018774817 scopus 로고
    • Morphological changes in membrane systems of Staphylococci after different fixation procedures
    • Suganuma, A. and H. Morioka (1979): Morphological changes in membrane systems of Staphylococci after different fixation procedures. J. Electron. Microsc., 28, 29-35.
    • (1979) J. Electron. Microsc. , vol.28 , pp. 29-35
    • Suganuma, A.1    Morioka, H.2
  • 35
    • 0028885164 scopus 로고
    • Flow cytometric analysis of coastal lagoon bacterioplankton and picophytoplankton: Fixation and storage effects
    • Troussellier, M., C. Courties and S. Zettelmaier (1995): Flow cytometric analysis of coastal lagoon bacterioplankton and picophytoplankton: fixation and storage effects. Estuar. Coast. Shelf Sci., 40, 621-633.
    • (1995) Estuar. Coast. Shelf Sci. , vol.40 , pp. 621-633
    • Troussellier, M.1    Courties, C.2    Zettelmaier, S.3
  • 36
    • 0032814029 scopus 로고    scopus 로고
    • Flow cytometric discrimination of bacterial populations in seawater based on SYTO 13 staining of nucleic acids
    • Troussellier, M., C. Courties, P. Lebaron and P. Servais (1999): Flow cytometric discrimination of bacterial populations in seawater based on SYTO 13 staining of nucleic acids. FEMS Microbiol. Ecol., 29, 319-330.
    • (1999) FEMS Microbiol. Ecol. , vol.29 , pp. 319-330
    • Troussellier, M.1    Courties, C.2    Lebaron, P.3    Servais, P.4
  • 37
    • 0026564045 scopus 로고
    • Effects of storage on direct estimates of bacterial numbers of preserved seawater samples
    • Turley, C. M. and D. J. Hughes (1992): Effects of storage on direct estimates of bacterial numbers of preserved seawater samples. Deep-Sea Res., 39, 375-394.
    • (1992) Deep-Sea Res. , vol.39 , pp. 375-394
    • Turley, C.M.1    Hughes, D.J.2
  • 38
    • 0035807127 scopus 로고    scopus 로고
    • Dynamics of whole community bacterial production and grazing losses in seawater incubations as related to the changes in the proportions of bacteria with different DNA content
    • Vaque, D., E. O. Casamayor and J. M. Gasol (2001): Dynamics of whole community bacterial production and grazing losses in seawater incubations as related to the changes in the proportions of bacteria with different DNA content. Aquat. Microb. Ecol., 25, 163-177.
    • (2001) Aquat. Microb. Ecol. , vol.25 , pp. 163-177
    • Vaque, D.1    Casamayor, E.O.2    Gasol, J.M.3
  • 39
    • 0032805947 scopus 로고    scopus 로고
    • A quantitative fluorescence-based microplate assay for the determination of double-stranded DNA using SYBR Green I and a standard ultraviolet transilluminator gel imaging system
    • Vitzthum, F., G. Geiger, H. Bisswanger, H. Brunner and J. Bernhagen (1999): A quantitative fluorescence-based microplate assay for the determination of double-stranded DNA using SYBR Green I and a standard ultraviolet transilluminator gel imaging system. Anal. Biochem., 276, 59-64.
    • (1999) Anal. Biochem. , vol.276 , pp. 59-64
    • Vitzthum, F.1    Geiger, G.2    Bisswanger, H.3    Brunner, H.4    Bernhagen, J.5
  • 40
    • 0001022353 scopus 로고    scopus 로고
    • Enumerating nucleoid-visible marine bacterioplankton: Bacterial abundance determined after storage of formalin fixed samples agrees with isopropanol rinsing method
    • Vosjan, J. H. and G. J. van Noort (1998): Enumerating nucleoid-visible marine bacterioplankton: Bacterial abundance determined after storage of formalin fixed samples agrees with isopropanol rinsing method. Aquat. Microb. Ecol., 14, 149-154.
    • (1998) Aquat. Microb. Ecol. , vol.14 , pp. 149-154
    • Vosjan, J.H.1    van Noort, G.J.2
  • 41
    • 0031736602 scopus 로고    scopus 로고
    • Utility of green fluorescent nucleic acid dyes and aluminum oxide membrane filters for rapid epifluorescence enumeration of soil and sediment bacteria
    • Weinbauer, M. G., C. Beckmann and M. G. Hofle (1998): Utility of green fluorescent nucleic acid dyes and aluminum oxide membrane filters for rapid epifluorescence enumeration of soil and sediment bacteria. Appl. Environ. Microbiol., 64, 5000-5003.
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 5000-5003
    • Weinbauer, M.G.1    Beckmann, C.2    Hofle, M.G.3
  • 42
    • 0034609891 scopus 로고    scopus 로고
    • Seasonal variation of two different heterotrophic bacterial assemblages in subarctic coastal seawater
    • Yanada, M., T. Yokokawa, C. W. Lee, H. Tanaka, I. Kudo and Y. Maita (2000): Seasonal variation of two different heterotrophic bacterial assemblages in subarctic coastal seawater. Mar. Ecol. Prog. Ser., 204, 289-292.
    • (2000) Mar. Ecol. Prog. Ser. , vol.204 , pp. 289-292
    • Yanada, M.1    Yokokawa, T.2    Lee, C.W.3    Tanaka, H.4    Kudo, I.5    Maita, Y.6
  • 43
    • 0035516025 scopus 로고    scopus 로고
    • Comparison of cellular and biomass specific activities of dominant bacterioplankton groups in stratified waters of the Celtic Sea
    • Zubkov, M. V., B. M. Fuchs, P. H. Burkill and R. Amann (2001): Comparison of cellular and biomass specific activities of dominant bacterioplankton groups in stratified waters of the Celtic Sea. Appl. Environ. Microbiol., 67, 5210-5218.
    • (2001) Appl. Environ. Microbiol. , vol.67 , pp. 5210-5218
    • Zubkov, M.V.1    Fuchs, B.M.2    Burkill, P.H.3    Amann, R.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.