Simultaneous measurement of metabolic activity and membrane integrity in marine bacterioplankton determined by confocal laser-scanning microscopy

Aquatic Microbial Ecology

Volumn 39, Issue 3, 2005, Pages 225-233

  Pirker, Heidemarie ^a   Pausz, Clemens ^a   Stoderegger, Karen E ^a   Herndl, Gerhard J ^a  

^a ROYAL NETHERLANDS INSTITUTE FOR SEA RESEARCH   (Netherlands)

Author keywords

Active versus dead; Bacteria; Metabolic activity; Microautoradiography; Propidium iodide method; Single cell analysis

Indexed keywords

EID: 22044441984     PISSN: 09483055     EISSN: None     Source Type: Journal    
DOI: 10.3354/ame039225     Document Type: Article

References (29)

1. Arrieta JM, Herndl GJ (2002) Changes in bacterial β-glucosidase diversity during a coastal phytoplankton bloom. Limnol Oceanogr 47:594-599
2. Berman T, Kaplan B, Chava S, Viner Y, Sherr BF, Sherr EB (2001) Metabolically active bacteria in Lake Kinneret. Aquat Microb Ecol 23:213-224
3. Carman KR (1990) Radioactive labeling of a natural assemblage of marine sedimentary bacteria and microalgae for trophic studies - an autoradiographic study. Microb Ecol 19:279-290
4. Choi JW, Sherr EB, Sherr BF (1996) Relation between presence and absence of a visible nucleoid and metabolic activity in bacterioplankton cells. Limnol Oceanogr 41:1161-1168
5. Choi JW, Sherr BF, Sherr EB (1999) Dead or alive? A large fraction of ETS-inactive marine bacterioplankton cells, as assessed by reduction of CTC, can become ETS-active with incubation and substrate addition. Aquat Microb Ecol 18:105-115
6. Cottrell MT, Kirchman DL (2003) Contribution of major bacterial groups to bacterial biomass production (thymidine and leucine incorporation) in the Delaware estuary. Limnol Oceanogr 48:168-178
7. Del Giorgio P, Scarborough G (1995) Increase in the proportion of metabolically active bacteria along gradients of enrichment in freshwater and marine plankton: implications for estimates of bacterial growth and production. J Plankton Res 17:1905-1924
8. Gasol JM, Del Giorgio PA (2000) Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. Sci Mar 64:197-224
9. Gregori G, Citterio S, Ghiani A, Labra M, Sgorbati S, Brown S, Denis M (2001) Resolution of viable and membrane-compromised bacteria in freshwater and marine waters based on analytical flow cytometry and nucleic acid double staining. Appl Environ Microbiol 67:4662-4670
10. Heissenberger A, Leppard G, Herndl GJ (1996) Relationship between the intracellular integrity and the morphology of the capsular envelope in attached and free-living marine bacteria. Appl Environ Microbiol 62:4521-4528
11. Hobbie J, Daley R, Jasper S (1977) Use of Nuclepore filters for counting bacteria by epifluorescence microscopy. Appl Environ Microbiol 33:1225-1228
12. Howard-Jones M, Frischer M, Verity P (2001) Determining the physiological status of individual bacterial cells. In: Paul JH (ed) Marine microbiology, Vol 30. Academic Press, San Diego, CA, p 176-205
13. Jernaes MW, Steen HB (1994) Staining of Escherichia coli for flow-cytometry - influx and efflux of ethidium-bromide. Cytometry 17:302-309
14. Karner M, Fuhrman J (1997) Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining. Appl Environ Microbiol 63:1208-1213
15. Lebaron P, Servais P, Agogue H, Courties C, Joux F (2001) Does the high nucleic acid content of individual bacterial cells allow us to discriminate between active cells and inactive cells in aquatic systems? Appl Environ Microbiol 67:1775-1782
16. Lopez-Amoros R, Comas J, Vives-Rego J (1995) Flow cytometric assessment of Escherichia coli and Salmonella typhimurium starvation-survival in seawater using rhodamine 123, propidium iodide, and oxonol. Appl Environ Microbiol 61:2521-2526
17. Lopez-Amoros R, Castel S, Comas-Riu J, Vives-Rego J (1997) Assessment of E. coli and Salmonella viability and starvation by confocal laser microscopy and flow cytometry using rhodamine 123, DiBAC4(3), propidium iodide, and CTC. Cytometry 29:298-305
18. Lovejoy C, Legendre L, Klein B, Tremblay JE, Ingram R, Therriault JC (1996) Bacterial activity during early winter mixing (Gulf of St Lawrence, Canada). Aquat Microb Ecol 10:1-13
19. Meyer-Reil L (1978) Autoradiography and epifluorescence microscopy combined for the determination of number and spectrum of actively metabolizing bacteria in natural waters. Appl Environ Microbiol 36:506-512
20. Pernthaler A, Preston CM, Pernthaler J, DeLong EF, Amann R (2002a) Comparison of fluorescently labeled oligonucleotide and polynucleotide probes for the detection of pelagic marine Bacteria and Archaea. Appl Environ Microbiol 68:661-667
21. Pernthaler A, Pernthaler J, Amann R (2002b) Fluorescence in situ hybridization and catalysed reporter deposition for the identification of marine Bacteria. Appl Environ Microbiol 68:3094-3101
22. Schumann R, Schiewer U, Karsten U, Rieling T (2003) Viability of bacteria from different aquatic habitats. II. Cellular fluorescent markers for membrane integrity and metabolic activity. Aquat Microb Ecol 32:137-150
23. Sherr BF, Del Giorgio P, Sherr EB (1999a) Estimating abundance and single-cell characteristics of respiring bacteria via the redox dye CTC. Aquat Microb Ecol 18:117-131
24. Sherr EB, Sherr BF, Sigmon CT (1999b) Activity of marine bacteria under incubated and in situ conditions. Aquat Microb Ecol 20:213-223
25. Smith EM, Del Giorgio AA (2003) Low fractions of active bacteria in natural aquatic communities? Aquat Microb Ecol 31:203-208
26. Steen HB, Jernaes MW, Skarstad K, Boye E (1994) Staining and measurement of DNA in bacteria. Methods in cell biology, Vol 42. Academic Press, San Diego, CA, p 477-487
27. Vives-Rego J, Lebaron P, Nebe-von Caron G (2000) Current and future applications of flow cytometry in aquatic microbiology. FEMS Microbiol Rev 24:429-448
28. Williams SC, Hong Y, Danavall DCA, Howard-Jones MH, Gibson D, Frischer ME, Verity PG (1998) Distinguishing between living and nonliving bacteria: evaluation of the vital stain propidium iodide and its combined use with molecular probes in aquatic samples. J Microbiol Methods 32:225-236
29. Zweifel U, Hagström A (1995) Total counts of marine bacteria include a large fraction of non-nucleoid-containing bacteria (ghosts). Appl Environ Microbiol 61:2180-2185