Enumerating nucleoid-visible marine bacterioplankton: Bacterial abundance determined after storage of formalin fixed samples agrees with isopropanol rinsing method

Aquatic Microbial Ecology

Volumn 14, Issue 2, 1998, Pages 149-154

  Vosjan, Jan H ^a   Van Noort, Govert J ^a  

^a ROYAL NETHERLANDS INSTITUTE FOR SEA RESEARCH   (Netherlands)

Author keywords

Active bacteria; Bacterioplankton; Enumeration; Epifluorescence microscopy; Fixation; Preservation

Indexed keywords

ALGAE; BACTERIA (MICROORGANISMS);

EID: 0001022353     PISSN: 09483055     EISSN: None     Source Type: Journal    
DOI: 10.3354/ame014149     Document Type: Article

References (23)

1. Ammerman JW, Fuhrman JA, Hagström Å, Azam F (1984) Bacterioplankton growth in seawater: I. Growth kinetics and cellular characteristics in seawater cultures. Mar Ecol Prog Ser 18:31-39
2. Azam F, Fenchel T, Field JG, Gray JS, Meyer-Reil LA, Thingstad F (1983) The ecological role of water-column microbes in the sea. Mar Ecol Prog Ser 10:257-263
3. Cadée GC, Hegeman J (1979) Phytoplankton primary production, chlorophyll and composition in an inlet of the Western Wadden Sea (Marsdiep). Neth J Sea Res 13: 224-241
4. Choi JW, Sherr EB, Sherr BF (1996) Relation between presence-absence of a visible nucleoid and metabolic activity in bacterioplankton cells. Limnol Oceanogr 41:1161-1168
5. Douglas DJ, Novitsky JA, Fournier RO (1987) Microautoradiography-based enumeration of bacteria with estimates of thymidine-specific growth and production rates. Mar Ecol Prog Ser 36:91-99
6. Grossmann S (1994) Bacterial activity in sea ice and open water of the Weddell Sea, Antarctica: a microautoradiographic study. Microb Ecol 28:1-18
7. Gundersen K, Bratbak G, Heldal M (1996) Factors influencing the loss of bacteria in preserved seawater samples. Mar Ecol Prog Ser 137:305-310
8. Heissenberger A, Leppard GG, Herndl GJ (1996) Relationship between the intracellular integrity and the morphology of the capsular envelope in attached and free-living marine bacteria. Appl Environ Microbiol 62:4521-4528
9. Hobbie JE, Daley RJ, Jasper S (1977) Use of Nuclepore filters for counting bacteria by epifluorescence microscopy. Appl Environ Microbiol 33:1225-1228
10. Hoppe HG (1977) Analysis of actively metabolizing bacterial populations. In: Rheinheimer G (ed) Microbial ecology of a brackish water environment. Springer-Verlag, Berlin, p 179-197
11. Hoppe HG (1978) Relations between active bacteria and heterotrophic potential in the sea. Neth J Sea Res 13:78-98
12. Karner M, Fuhrman JA (1997) Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining. Appl Environ Microbiol 63:1208-1213
13. Meyer-Reil LA (1978) Autoradiography and epifluorescence microscopy combined for the determination of number and spectrum of actively metabolizing bacteria in natural waters. Appl Environ Microbiol 36:506-512
14. Porter J, Diaper J, Edwards C, Pickup R (1995) Direct measurements of natural planktonic bacterial community viability by flow cytometry. Appl Environ Microbiol 61: 2783-2786
15. Porter KG, Feig YS (1980) The use of DAPI for identifying and counting aquatic microflora. Limnol Oceanogr 25:943-948
16. Posch T, Pernthaler J, Afreider A, Psenner R (1997) Cell-specific respiratory activity of aquatic bacteria studied with the tetrazolium reduction method, Cyto-clear slides, and image analysis. Appl Environ Microbiol 63:867-873
17. Pyle BH, Broadaway SC, McFeters GA (1995) Factors affecting the determination of respiratory activity on the basis of cyanoditolyl tetrazolium chloride reduction with membrane filtration. Appl Environ Microbiol 61:4304-4309
18. Tabor PS, Neihof RA (1982) Improved method for determination of respiring individual microorganisms in natural waters. Appl Environ Microbiol 43:1249-1255
19. Turley CM, Hughes DJ (1992) Effects of storage on direct estimates of bacterial numbers of preserved seawater samples. Deep Sea Res 39:375-394
20. Turley CM, Hughes DJ (1994) The effect of storage temperature on the enumeration of epifluorescence-detectable bacterial cells in preserved seawater. J Mar Biol Assoc UK 74:259-262
21. Zimmermann R, Iturriaga R, Becker-Birck J (1978) Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl Environ Microbiol 36:926-935
22. Zimmermann R, Meyer-Reil LA (1974) A new method for fluorescence staining of bacterial populations on membrane filters. Kieler Meeresforsch 30:24-27
23. Zweifel UL, Hagström Å (1995) Total counts of marine bacteria include a large fraction of non-nucleoid-containing bacteria (ghosts). Appl Environ Microbiol 61:2180-2185