Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria

Aquatic Microbial Ecology

Volumn 14, Issue 2, 1998, Pages 113-118

  Noble, Rachel T ^a   Fuhrman, Jed A ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

Epifluorescence; Marine ecology; SYBR Green I; Virus

Indexed keywords

BACTERIA (MICROORGANISMS); VISCUM ALBUM;

EID: 0001679973     PISSN: 09483055     EISSN: None     Source Type: Journal    
DOI: 10.3354/ame014113     Document Type: Article

References (22)

1. Bergh O, Børsheim KY, Bratbak G, Heldal M (1989) High abundance of viruses found in aquatic environments. Nature 340:467-468
2. Børsheim KY, Bratbak G, Heldal M (1990) Enumeration and biomass estimation of planktonic bacteria and viruses by transmission electron microscopy. Appl Environ Microbiol 56:352-356
3. Bratbak G, Heldal M, Norland S, Thingstad TF (1990) Viruses as partners in spring bloom microbial trophodynamics. Appl Environ Microbiol 56:1400-1405
4. Cochlan WP, Wikner J, Steward GF, Smith DC, Azam F (1993) Spatial distribution of viruses, bacteria and chlorophyll a in neritic, oceanic and estuarine environments. Mar Ecol Prog Ser 92:77-87
5. Frank H, Moebus K (1987) An electron microscopic study of bacteriophages from marine waters. Helgol Meeresunters 41:385-414
6. Fuhrman JA, Noble RT (1995) Viruses and protists cause similar bacterial mortality in coastal seawater. Limnol Oceanogr 40(7):1236-1242
7. Fuhrman JA, Suttle CA (1993) Viruses in marine planktonic systems. Oceanography 6:51-63
8. Fuhrman JA, Wilcox RM, Noble RT, Law NC (1993) Viruses in marine food webs. In: Guerrero R, Pedrós-Alió C (eds) Trends in microbial ecology. Spanish Society for Microbiology, Barcelona, p 295-298
9. Hara S, Terauchi K, Koike I (1991) Abundance of viruses in marine waters: assessment by epifluorescence and transmission electron microscopy. Appl Environ Microbiol 57(9): 2731-2734
10. Hennes KP, Suttle CA (1995) Direct counts of Viruses in natural waters and laboratory cultures by epifluorescence microscopy. Limnol Oceanogr 40(6):1050-1055
11. Hobbie JE, Daley RJ, Jasper S (1977) Use of Nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol 33:1225-1228
12. Marie D, Partensky F, Jacquet S, Vaulot D (1997) Enumeration and cell-cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic-acid stain SYBR green I. Appl Environ Microbiol 63(1):186-193
13. Mathews J, Buthala DA (1970) Centrifugal sedimentation of virus particles for electron microscope counting. J Virol 5(5):598-603
14. Paul JH, Jiang SC, Rose JB (1991) Concentration of viruses and dissolved DNA from aquatic environments by vortex flow filtration. Appl Environ Microbiol 57(8):2197-2204
15. Proctor LM, Fuhrman JA (1990) Viral mortality of marine bacteria and cyanobacteria. Nature 343:60-62
16. Proctor LM, Fuhrman JA (1992) Mortality of marine bacteria in response to enrichments of the virus size fraction from seawater. Mar Ecol Prog Ser 87:283-293
17. Stockner JG, Klut ME, Cochlan WP (1990) Leaky filters: a warning to aquatic ecologists. Can J Fish Aquat Sci 47: 16-23
18. Suttle CA (1993) Enumeration and isolation of marine viruses. In: Kemp PF, Sherr BF, Sherr EB, Cole JJ (eds) Current methods in aquatic microbial ecology. Lewis Publishing, Boca Raton, p 121-134
19. Suttle CA, Chan AM, Cottrell MT (1990) Infection of phytoplankton by viruses and reduction of primary productivity. Nature 387:467-469
20. Weinbauer MG, Suttle CA (1997) Comparison of epifluorescence and transmission electron microscopy for counting viruses in natural marine waters. Aquat Microb Ecol 13: 225-232
21. Wommack KE, Hill RT, Kessel M, Russek-Cohen E, Colwell RR (1992) Distribution of viruses in Chesapeake Bay. Appl Environ Microbiol 58:2965-2970
22. Xenopolous MA, Bird DF (1997) Virus à la sauce Yo-Pro: Microwave enhanced staining for counting viruses by epi-fluorescence microscopy. Limnol Oceanogr 42(7)