The in vitro characterization of the iterative avermectin glycosyltransferase AveBI reveals reaction reversibility and sugar nucleotide flexibility

Journal of the American Chemical Society

Volumn 128, Issue 51, 2006, Pages 16420-16421

  Zhang, Changsheng ^a   Albermann, Christoph ^a   Fu, Xun ^a   Thorson, Jon S ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGLYCONE; AVERMECTIN; AVERMECTIN GLYCOSYLTRANSFERASE; CARBOHYDRATE; GLYCOSYLTRANSFERASE; MACROLIDE; SUGAR NUCLEOTIDE; UNCLASSIFIED DRUG;

ADDITION REACTION; ANTIBIOTIC BIOSYNTHESIS; ARTICLE; CATALYSIS; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME SPECIFICITY; GLYCOSYLATION; IN VITRO STUDY; STREPTOMYCES LIVIDANS;

CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME ACTIVATION; GLYCOSYLTRANSFERASES; IVERMECTIN; MOLECULAR CONFORMATION; NUCLEOTIDES; SENSITIVITY AND SPECIFICITY; STEREOISOMERISM;

EID: 33845935524     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja065950k     Document Type: Article

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19. 2. Reversibility of AveBI reaction was assayed by co-incubation of 100 μM avermectin Bla (1) or ivermectin (7) and 2 mM TDP with 12 μM AveBI at 30 °C overnight.
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