Expanding pyrimidine diphosphosugar libraries via structure-based nucleotidylyltransferase engineering

Proceedings of the National Academy of Sciences of the United States of America

Volumn 99, Issue 21, 2002, Pages 13397-13402

  Barton, William A ^a,c   Biggins, John B ^b,c   Jiang, Jiqing ^b   Thorson, Jon S ^b,c   Nikolov, Dimitar B ^a,c  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c Weill Cornell Graduate School of Biomedical Sciences   (United States)

Author keywords

Enzyme; Glycorandomization; Glycosyltransferase; Rational design; X ray crystallography

Indexed keywords

NUCLEOTIDYLTRANSFERASE; PYRIMIDINE DERIVATIVE; SUGAR PHOSPHATE; THYMIDINE DIPHOSPHATE; URIDINE DIPHOSPHATE;

AMINO ACID SUBSTITUTION; ARTICLE; BIODIVERSITY; BIOENGINEERING; CONTROLLED STUDY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; SALMONELLA ENTERICA; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DRUG DESIGN; ESCHERICHIA COLI; GLYCOSYLATION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; NUCLEOTIDYLTRANSFERASES; PROTEIN ENGINEERING; PYRIMIDINE NUCLEOTIDES; RECOMBINANT PROTEINS; SALMONELLA ENTERICA; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; NEGIBACTERIA; SALMONELLA; SALMONELLA ENTERICA;

EID: 0037109032     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.192468299     Document Type: Article

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