Selective modification of surface-exposed thiol groups in Trigonopsis variabilis D-amino acid oxidase using poly(ethylene glycol) maleimide and its effect on activity and stability of the enzyme

Biotechnology and Bioengineering

Volumn 96, Issue 1, 2007, Pages 9-17

  Slavica, Anita ^a,b   Dib, Iskandar ^a,b   Nidetzky, Bernd ^a,b  

^a RESEARCH CENTRE APPLIED BIOCATALYSIS   (Austria)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

(Thermo) stabilizations; Cofactor dissociation; Cysteine modification; D amino acid oxidase; FAD; PEG conjugate

Indexed keywords

(THERMO) STABILIZATIONS; COFACTOR DISSOCIATION; CYSTEINE MODIFICATION; D-AMINO ACID OXIDASE; FAD; PEG-CONJUGATE;

AMINO ACIDS; CATALYSIS; DISSOCIATION; ENZYMES; POLYETHYLENE GLYCOLS; THERMODYNAMIC STABILITY;

ALCOHOLS;

2,6 DICHLOROPHENOLINDOPHENOL; DEXTRO AMINO ACID OXIDASE; FLAVINE ADENINE NUCLEOTIDE; MACROGOL; MACROGOL MALEIMIDE; MALEIMIDE DERIVATIVE; METHIONINE; OXYGEN; THIOL GROUP; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; CONJUGATE; COVALENT BOND; DEAMINATION; DERIVATIZATION; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME STABILITY; NONHUMAN; OXIDATION; STEADY STATE; SURFACE PROPERTY; TEMPERATURE; THERMOSTABILITY; TRIGONOPSIS VARIABILIS; YEAST;

D-AMINO-ACID OXIDASE; ENZYME ACTIVATION; ENZYME STABILITY; MALEIMIDES; POLYETHYLENE GLYCOLS; SACCHAROMYCETALES; SULFHYDRYL COMPOUNDS; SURFACE PROPERTIES;

TRIGONOPSIS VARIABILIS;

EID: 33845924496     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21181     Document Type: Article

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