Expression of recombinant proteins lacking methionine as N-terminal amino acid in plastids: Human serum albumin as a case study

Journal of Biotechnology

Volumn 127, Issue 4, 2007, Pages 593-604

  Fernández San Millán, Alicia ^a   Farran, Inmaculada ^a   Molina, Andrea ^a   Mingo Castel, Angel M ^a   Veramendi, Jon ^a  

^a PUBLIC UNIVERSITY OF NAVARRE   (Spain)

Author keywords

Human serum albumin; Methionine removal; N end rule; Plastid transformation; Post translational modifications; Transit peptide

Indexed keywords

AMINO ACIDS; GENETIC ENGINEERING; PLANT CELL CULTURE;

HUMAN SERUM ALBUMIN; METHIONINE REMOVAL; N-END RULE; PLASTID TRANSFORMATION; POST-TRANSLATIONAL MODIFICATIONS; TRANSIT PEPTIDE;

PROTEINS;

METHIONINE; METHIONYL AMINOPEPTIDASE; RECOMBINANT PROTEIN; RIBULOSEBISPHOSPHATE CARBOXYLASE; SERUM ALBUMIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CHLOROPLAST; HUMAN; NONHUMAN; PLASTID; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN STABILITY; TOBACCO;

AMINO ACID SEQUENCE; CHLOROPLASTS; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; HUMANS; METHIONINE; MOLECULAR SEQUENCE DATA; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; RIBULOSE-BISPHOSPHATE CARBOXYLASE; SERUM ALBUMIN; TOBACCO;

NICOTIANA TABACUM;

EID: 33845645433     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.08.010     Document Type: Article

References (46)

1. Arokiaraj P., Ruker F., Obermayr E., Shamsul Bahri A.R., Hafsah J., Carter D.C., and Yeang H.Y. Expression of human serum albumin in transgenic Hevea Brasiliensis. Malaysian Rubber Borrad 5 (2002) 157-166
2. Ben-Bassat A., Bauer K., Chang S.Y., Myambo K., Boosman A., and Chang S. Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure. J. Bacteriol. 169 (1987) 751-757
3. Berry-Lowe S., and Schmidt B.J. Chloroplast protein transport. In: Bogorad L., and Vasil I.K. (Eds). The Molecular Biology of Plastids and Mitochondria (1991), Academic Press, Orlando 257-301
4. Bruce B.D. The paradox of plastid transit peptides: conservation of function despite divergence in primary structure. Biochim. Biophys. Acta 1541 (2001) 2-21
5. Daniell H. Transformation and foreign gene expression in plants by microprojectile bombardment. Meth. Mol. Biol. 62 (1997) 463-489
6. Daniell H., Chebolu S., Kumar S., Singleton M., and Falconer R. Chloroplast-derived vaccine antigens and other therapeutic proteins. Vaccine 23 (2005) 1779-1783
7. Daniell H., Khan M.S., and Allison L. Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology. Trends Plant Sci. 7 (2002) 84-91
8. Dieryck W., Pagnier J., Poyart C., Marden M.C., Gruber V., Bournat P., Baudino S., and Merot B. Human haemoglobin from transgenic tobacco. Nature 386 (1997) 29-30
9. Emanuelsson O., Nielsen H., and von Heijne G. ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci. 8 (1999) 978-984
10. Erhard M.H., Kellner J., Schmidhuber S., Schams D., and Losch U. Identification of antigenic differences of recombinant and pituitary bovine growth hormone using monoclonal antibodies. J. Immunoassay 15 (1994) 1-19
11. Farran I., Sanchez-Serrano J.J., Medina J.F., Prieto J., and Mingo-Castel A.M. Targeted expression of human serum albumin to potato tubers. Transgenic Res. 11 (2002) 337-346
12. Fernández-San Millán A., Mingo-Castel A.M., Miller M., and Daniell H. A chloroplast transgenic approach to hyper-express and purify human serum albumin, a protein susceptible to proteolytic degradation. Plant Biotech. J. 1 (2003) 71-79
13. Giglione C., and Meinnel T. Organellar peptide deformylases: universality of the N-terminal methionine cleavage mechanism. Trends Plant Sci. 6 (2001) 566-572
14. Giglione C., Vallon O., and Meinnel T. Control of protein life-span by N-terminal methionine excision. Embo J. 22 (2003) 13-23
15. Gomez S.M., Bil K.Y., Aguilera R., Nishio J.N., Faull K.F., and Whitelegge J.P. Transit peptide cleavage sites of integral thylakoid membrane proteins. Mol. Cell Proteomics 2 (2003) 1068-1085
16. Gomez S.M., Nishio J.N., Faull K.F., and Whitelegge J.P. The chloroplast grana proteome defined by intact mass measurements from liquid chromatography mass spectrometry. Mol. Cell Proteomics 1 (2002) 46-59
17. Hirel P.H., Schmitter M.J., Dessen P., Fayat G., and Blanquet S. Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid. Proc. Natl. Acad. Sci. U.S.A. 86 (1989) 8247-8251
18. Houtz R.L., Stults J.T., Mulligan R.M., and Tolbert N.E. Post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase. Proc. Natl. Acad. Sci. U.S.A. 86 (1989) 1855-1859
19. Knight J.S., Madueno F., and Gray J.C. Import and sorting of proteins by chloroplasts. Biochem. Soc. Trans. 21 (1993) 31-36
20. Kusnadi A., Nikolov Z., and Howard J. Production of recombinant proteins in transgenic plants: practical considerations. Biotechnol. Bioeng. 56 (1997) 473-484
21. Latta M., Knap M., Sarmientos P., Brefort G., Becquart J., Guerrier L., Jung G., and Mayaux J.F. Synthesis and purification of mature human serum albumin from Escherichia coli. Bio/Technology 5 (1987) 1309-1314
22. Leelavathi S., Gupta N., Maiti S., Ghosh A., and Reddy V.S. Overproduction of an alkali and thermostable xylanase in tobacco chloroplasts and efficient recovery of the enzyme. Mol. Breeding 11 (2003) 59-67
23. Maliga P. Progress towards commercialization of plastid transformation technology. Trends Biotechnol. 21 (2003) 20-28
24. Maliga P. Plastid transformation in higher plants. Annu. Rev. Plant Biol. 55 (2004) 289-313
25. Martin W., Rujan T., Richly E., Hansen A., Cornelsen S., Lins T., Leister D., Stoebe B., Hasegawa M., and Penny D. Evolutionary analysis of Arabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleus. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 12246-12251
26. Molina A., Hervas-Stubbs S., Daniell H., Mingo-Castel A.M., and Veramendi J. High-yield expression of a viral peptide animal vaccine in transgenic tobacco chloroplasts. Plant Biotech. J. 2 (2004) 141-153
27. Nawrath C., Poirier Y., and Somerville C. Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of A. thaliana results in high levels of polymer accumulation. Proc. Natl. Acad. Sci. U.S.A. 91 (1994) 12760-12764
28. Newton D.L., Boque L., Wlodawer A., Huang C.Y., and Rybak S.M. Single amino acid substitutions at the N-terminus of a recombinant cytotoxic ribonuclease markedly influence biochemical and biological properties. Biochemistry 37 (1998) 5173-5183
29. Peters T.J. All About albumin (1995), Academic Press, San Diego
30. Proudfoot A.E., Power C.A., Hoogewerf A.J., Montjovent M.O., Borlat F., Offord R.E., and Wells T.N. Extension of recombinant human RANTES by the retention of the initiating methionine produces a potent antagonist. J. Biol. Chem. 271 (1996) 2599-2603
31. Richter S., and Lamppa G.K. A chloroplast processing enzyme functions as the general stromal processing peptidase. Proc. Natl. Acad. Sci. U.S.A. 95 (1998) 7463-7468
32. Richter S., and Lamppa G.K. Stromal processing peptidase binds transit peptides and initiates their ATP-dependent turnover in chloroplasts. J. Cell Biol. 147 (1999) 33-44
33. Schlott B., Hartmann M., Guhrs K.H., Birch-Hirschfeid E., Pohl H.D., Vanderschueren S., Van de Werf F., Michoel A., Collen D., and Behnke D. High yield production and purification of recombinant staphylokinase for thrombolytic therapy. Biotechnology (NY) 12 (1994) 185-189
34. Sherman F., Stewart J.W., and Tsunasawa S. Methionine or not methionine at the beginning of a protein. Bioessays 3 (1985) 27-31
35. Staub J.M., García B., Graves J., Hajdukiewicz P.T.J., Hunter P., Nehra N.S., Paradkar V., Schlittler M., Carroll J.A., Spatola L., Ward D., Ye G., and Russell D.A. High-level production of a human therapeutic protein in tobacco chloroplasts. Nat. Biotechnol. 18 (2000) 333-338
36. Theg S.M., and Scott S.V. Protein import into chloroplasts. Trends Cell Biol. 3 (1993) 186-190
37. Tregoning J.S., Nixon P., Kuroda H., Svab Z., Clare S., Bowe F., Fairweather N., Ytterberg J., van Wijk K.J., Dougan G., and Maliga P. Expression of tetanus toxin fragment C in tobacco chloroplasts. Nucl. Acids Res. 31 (2003) 1174-1179
38. VanderVere P.S., Bennett T.M., Oblong J.E., and Lamppa G.K. A chloroplast processing enzyme involved in precursor maturation shares a zinc-binding motif with a recently recognized family of metalloendopeptidases. Proc. Natl. Acad. Sci. U.S.A. 92 (1995) 7177-7181
39. Varshavsky A. The N-end rule: functions, mysteries, uses. Proc. Natl. Acad. Sci. U.S.A. 93 (1996) 12142-12149
40. Watson J., Koya V., Leppla S.H., and Daniell H. Expression of Bacillus anthracis protective antigen in transgenic chloroplasts of tobacco, a non-food/feed crop. Vaccine 22 (2004) 4374-4384
41. Whitney S.M., and Andrews T.J. The gene for the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit relocated to the plastid genome of tobacco directs the synthesis of small subunits that assemble into Rubisco. Plant Cell 13 (2001) 193-205
42. Whitney S.M., and Andrews T.J. Plastome-encoded bacterial ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) supports photosynthesis and growth in tobacco. Proc. Natl. Acad. Sci. U.S.A. 98 (2001) 14738-14743
43. Whitney S.M., Baldet P., Hudson G.S., and Andrews T.J. Form I Rubiscos from non-green algae are expressed abundantly but not assembled in tobacco chloroplasts. Plant J. 26 (2001) 535-547
44. Yadava U.L. A rapid and nondestructive method to determine chlorophyll content in intact leaves. Hortscience 21 (1986) 1449-1450
45. Yamaguchi K., and Subramanian A.R. The plastid ribosomal proteins. Identification of all the proteins in the 50 S subunit of an organelle ribosome (chloroplast). J. Biol. Chem. 275 (2000) 28466-28482
46. Yamaguchi K., von Knoblauch K., and Subramanian A.R. The plastid ribosomal proteins. Identification of all the proteins in the 30 S subunit of an organelle ribosome (chloroplast). J. Biol. Chem. 275 (2000) 28455-28465