Single amino acid substitutions at the N-terminus of a recombinant cytotoxic ribonuclease markedly influence biochemical and biological properties

Biochemistry

Volumn 37, Issue 15, 1998, Pages 5173-5183

  Newton, Dianne L ^b   Boque, Lluis ^a   Wlodawer, Alexander ^a   Huang, Charles Y ^c   Rybak, Susanna M ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b SAIC FREDERICK INC   (United States)

^c NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BREFELDIN A; MONENSIN; RIBONUCLEASE;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANTINEOPLASTIC ACTIVITY; ARTICLE; CYTOTOXICITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; PROTEIN SYNTHESIS INHIBITION; STRUCTURE ANALYSIS;

ANIMALS; ANTINEOPLASTIC AGENTS; CELL-FREE SYSTEM; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG DESIGN; EGG PROTEINS; HUMANS; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; PROTEIN SYNTHESIS INHIBITORS; RABBITS; RECOMBINANT PROTEINS; RETICULOCYTES; RIBONUCLEASES; RNA, TRANSFER; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TUMOR CELLS, CULTURED;

ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS;

EID: 0032515910     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi972147h     Document Type: Article

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