Adsorption of human IgG on to poly(N-isopropylacrylamide)-based polymer particles

Biotechnology Letters

Volumn 28, Issue 24, 2006, Pages 2019-2025

  Silva, Claudia S O ^a   Baptista, Ricardo P ^a   Santos, Andrea M ^a   Martinho, José M G ^a   Cabral, Joaquim M S ^a   Taipa, M Ângela ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Adsorption; Human immunoglobulin G; Poly(methyl methacrylate); Poly(N isopropylacrylamide); Thermosensitive polymers

Indexed keywords

ADSORPTION; AMINES; GELS; IMMUNOLOGY; PH EFFECTS; PROTEINS; SYNTHESIS (CHEMICAL);

HUMAN IMMUNOGLOBULIN G (HIGG); POLY METHYL METHACRYLATE; POLY N ISOPROPYLACRYLAMIDE; THERMOSENSITIVE POLYMERS;

ORGANIC POLYMERS;

ACRYLAMIDE DERIVATIVE; IMMUNOGLOBULIN G; NANOPARTICLE; POLY(N ISOPROPYLACRYLAMIDE); POLY(N-ISOPROPYLACRYLAMIDE); POLYMER;

ADSORPTION; ARTICLE; CHEMISTRY; EVALUATION; HUMAN; METABOLISM; PH; TEMPERATURE; TIME;

ACRYLAMIDES; ADSORPTION; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; NANOPARTICLES; POLYMERS; TEMPERATURE; TIME FACTORS;

EID: 33845297264     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9188-2     Document Type: Article

References (18)

1. Baptista RP, Santos AM, Fedorov A, Martinho JM, Pichot C, Elaissari A, Cabral JM, Taipa MA (2003) Activity, conformation and dynamics of cutinase adsorbed on poly(methyl methacrylate) latex particles. J Biotechnol 102(3):241-249
2. Duracher D, Sauzedde F, Elaissari A, Perrin A, Pichot C (1998a) Cationic amino-containing N-isopropylacrylamide-styrene copolymer latex particles: 1-Particle size and morphology vs. polymerization process. Colloid Polym Sci 276(3):219-231
3. Fong RB, Ding Z, Hoffman AS, Stayton PS (2002) Affinity separation using an Fv antibody fragment-"smart" polymer conjugate. Biotechnol Bioeng 79(3):271-276
4. Galaev IY, Mattiasson B (1999) 'Smart' polymers and what they could do in biotechnology and medicine. Trends Biotechnol 17(8):335-340
5. Gil ES, Hudson SA (2004) Stimuli-reponsive polymers and their bioconjugates. Prog Polym Sci 29(12):1173-1222
6. Hilbrig F, Freitag R (2003) Protein purification by affinity precipitation. J Chromatogr B Analyt Technol Biomed Life Sci 790(1-2):79-90
7. Hoffman AS (2000) Bioconjugates of intelligent polymers and recognition proteins for use in diagnostics and affinity separations. Clin Chem 46(9):1478-1486
8. Hoffman AS (2002) Hydrogels for biomedical applications. Adv Drug Deliv Rev 54(1):3-12
9. Kawaguchi H, Fujimoto K, Mizuhara Y (1992) Hydrogel microspheres.3. temperature-dependent adsorption of proteins on poly-N-isopropylacrylamide hydrogel microspheres. Colloid Polym Sci 270(1):53-57
10. Kim JY, Mulchandani A, Chen W (2005) Temperature-triggered purification of antibodies. Biotechnol Bioeng 90(3):373-379
11. Kumar A, Wahlund PO, Kepka C, Galaev IY, Mattiasson B (2003) Purification of histidine-tagged single-chain Fv-antibody fragments by metal chelate affinity precipitation using thermoresponsive copolymers. Biotechnol Bioeng 84(4):494-503
12. Pelton R (2000) Temperature-sensitive aqueous microgels. Adv Colloid Interface Sci 85(1):1-33
13. Roque AC, Lowe CR, Taipa MA (2004) Antibodies and genetically engineered related molecules: production and purification. Biotechnol Prog 20(3):639-654
14. Roque AC, Taipa MA, Lowe CR (2005a) An artificial protein L for the purification of immunoglobulins and fab fragments by affinity chromatography. J Chromatogr A 1064(2):157-167
15. Roque AC, Taipa MA, Lowe CR (2005b) Synthesis and screening of a rationally designed combinatorial library of affinity ligands mimicking protein L from Peptostreptococcus magnus. J Mol Recognit 18(3):213-224
16. Santos AM, Elaissari A, Martinho JMG, Pichot C (2005) Synthesis of cationic poly(methyl methacrylate)-poly(N-isopropyl acrylamide) core-shell latexes via two-stage emulsion copolymerization. Polymer 46(4):1181-1188
17. Taipa MA, Kaul RH, Mattiasson B, Cabral JM (2000) Recovery of a monoclonal antibody from hybridoma culture supernatant by affinity precipitation with Eudragit S-100. Bioseparation 9(5):291-298
18. Taniguchi T, Duracher D, Delair T, Elaissari A, Pichot C (2003) Adsorption/desorption behavior and covalent grafting of an antibody onto cationic amino-functionalized poly(styrene-N-isopropylacrylamide) core-shell latex particles. Colloids Surf B 29(1):53-65