Purification of Histidine-Tagged Single-Chain Fv-Antibody Fragments by Metal Chelate Affinity Precipitation Using Thermoresponsive Copolymers

Biotechnology and Bioengineering

Volumn 84, Issue 4, 2003, Pages 494-503

  Kumar, Ashok ^a   Wahlund, Per Olof ^a   Kepka, Cecilia ^a   Galaev, Igor Yu ^a   Mattiasson, Bo ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Affinity macroligands; Bioseparation; His6 scFv fragments; Metal chelate affinity precipitation; Thermoprecipitation; Thermore sponsive copolymers

Indexed keywords

COPOLYMERS; POLYMERIZATION; PRECIPITATION (CHEMICAL); PURIFICATION; SYNTHESIS (CHEMICAL);

RADICAL POLYMERIZATION;

ANTIBODIES;

COPOLYMER; COPPER ION; EDETIC ACID; HISTIDINE TAGGED SINGLE CHAIN FV ANTIBODY FRAGMENT; IMIDAZOLE DERIVATIVE; IMMUNOGLOBULIN FRAGMENT; LIGAND; METAL AFFINITY MACROLIGAND; METAL CHELATE; METAL ION; NICKEL; POLY(N ISOPROPYLACRYLAMIDE); POLYMER; UNCLASSIFIED DRUG; VINYLIMIDAZOLE;

ARTICLE; BINDING AFFINITY; BIOSEPARATION; CHELATION; ESCHERICHIA COLI; FERMENTATION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INTERMETHOD COMPARISON; METAL CHELATE AFFINITY PRECIPITATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERIZATION; PRECIPITATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN POLYMER COMPLEX; PROTEIN PURIFICATION;

ACRYLIC RESINS; AFFINITY LABELS; CHELATING AGENTS; CHROMATOGRAPHY, AFFINITY; COPPER; HEAT; HISTIDINE; IMIDAZOLES; IMMUNOGLOBULIN FRAGMENTS; METALS; NICKEL; POLYMERS; PRECIPITATION;

EID: 0142104893     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10810     Document Type: Article

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