Activity, conformation and dynamics of cutinase adsorbed on poly(methyl methacrylate) latex particles

Journal of Biotechnology

Volumn 102, Issue 3, 2003, Pages 241-249

  Baptista, R P ^a   Santos, A M ^a   Fedorov, A ^a   Martinho, J M G ^a   Pichot, C ^b   Elaissari A ^b   Cabral, J M S ^a   Taipa, M A ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b UNIVERSITÉ DE LYON   (France)

Author keywords

Adsorption; Circular dichroism; Cutinase; Fluorescence; Lipolytic activity; PMMA

Indexed keywords

ADSORPTION; CATALYST ACTIVITY; ISOTHERMS; MOLECULES; POLYMETHYL METHACRYLATES;

CUTINASE;

ENZYMES;

CUTINASE; FUNGAL ENZYME; LATEX; PEPTIDE; POLY(METHYL METHACRYLATE); PROTEIN; TRYPTOPHAN;

ACIDITY; ADSORPTION; ALKALINITY; ALPHA HELIX; ANISOTROPY; ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; CORRELATION ANALYSIS; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME SPECIFICITY; FLUORESCENCE; FUSARIUM SOLANI; ISOTHERM; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; TEMPERATURE DEPENDENCE; ULTRAVIOLET RADIATION;

FUSARIUM; FUSARIUM SOLANI;

EID: 0037688019     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(03)00033-6     Document Type: Article

References (19)

1. Baptista R.P., Cabral J.M.S., Melo E.P. Trehalose delays the reversible but not the irreversible thermal denaturation of cutinase. Biotechnol. Bioeng. 70:(6):2000;699-703.
2. Brocklehurst K. Electrochemical Assays: The pH-Stat, Enzymic Assays: A Practical Approach. 1995;191-205 IRL Press, Oxford.
3. Carvalho C.M.L., Aires-Barros M.R., Cabral J.M.S. Cutinase: from molecular level to bioprocess development. Biotechnol. Bioeng. 66:(1):1999;17-34.
4. Gitlesen T., Bauer M., Adlercreutz P. Adsorption of lipase on propylene powder. Biochem. Biophys. Acta. 1345:1997;188-196.
5. Kleijn M., Norde W. The adsorption of proteins from aqueous solution on solid surfaces. Heterogen. Chem. Rew. 2:1995;157-172.
6. Koops B.C., Verheij H.M., Slotboom A.J., Egmond M.R. Effect of chemical modification on the activity of lipases in organic solvents. Enzyme Microb. Technol. 25:1999;622-631.
7. Lauwereys M., de Geus P., de Meutter J., Stranssens P., Matthyssens G. Schmid R.D., Alberghina L., Verger R. GBF Monographs. 16:1991;243-251 VCH, Weinheim.
8. Longhi S., Cambillau C. Structure-activity of cutinase, a small lipolytic enzyme. Biochem. Biophys. Acta. 1441:1999;185-196.
9. Martinho, J.M.G., Santos, A.M., Fedorov, A., Baptista, R.P., Taipa, M.A., Cabral, J.M.S., 2003. Tryptophan fluorescence of cutinase, influence of temperature, pH and light irradiation, submitted for publication.
10. Melo E.P., Faria T.Q., Martins L.O., Gonçalves A.M., Cabral J.M.S. Cutinase unfolding and stabilization by trehalose and mannosylglycerate. Poteins Struct. Funct. Genet. 42:2001;542-552.
11. Norde W., Zoungrana T. Surface-induced changes in the structure and activity of enzymes physically immobilized at solid-liquid interfaces. Biotechnol. Appl. Biochem. 28:1998;133-143.
12. Petersen S.B., Fojan P., Petersen Evamaria I., Petersen M.T.N. The thermal stability of Fusarium solani pisi cutinase as a function of pH. J. Biomed. Biotechnol. 1:(2):2001;62-69.
13. Prompers J.J., Groenberg A., Hilbers C.W., Pepermans H.A.M. Backbone dynamics of Fusarium solani pisi cutinase probed by nuclear magnetic resonance; the lack of interfacial activation revisited. Biochemistry. 38:1999;5315-5327.
14. Santos, A.M., Martinho, J.M.G., Pichot, C., Elaïssari, A., 2003. Preparation of low-size monodisperse carboxylic charged polymethyl(meta)acrylate latexes. Prog. Coll. Pol. Sci., in press.
15. Sebastião M.J., Cabral J.M.S., Aires-Barros M.R. Improved purification of a Fusarium solani pisi recombinant cutinase by phase partitioning in aqueous two-phase systems of polyethylene glycol and phosphate. Enzyme Microb. Technol. 18:1996;251-260.
16. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:1985;76-85.
17. Sivakumar M., Panduranga R. Synthesis, characterization, and in vitro release of ibuprofen from poly(MMA-HEMA) copolymeric core-shell hydrogel microspheres for biomedical applications. J. Appl. Polym. Sci. 83:2002;3045-3054.
18. Wahlgren M., Arnebrant T. Protein adsorption to solid surfaces. TIBTECH. 9:1991;201-208.
19. Yoon J.-Y., Park H.-Y., Kim J.-H., Kim W.-S. Adsorption of BSA on highly carboxylated microspheres-quantitative effects of surface functional groups and interaction forces. J. Coll. Interf. Sci. 177:1996;613-620.