Temperature-triggered purification of antibodies

Biotechnology and Bioengineering

Volumn 90, Issue 3, 2005, Pages 373-379

  Kim, Jae Young ^a,b   Mulchandani, Ashok ^a   Chen, Wilfred ^a  

^a University of California Riverside   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Affinity precipitation; Protein A; Protein purification

Indexed keywords

BINDING ENERGY; BIOTECHNOLOGY; CELL CULTURE; CHELATION; PRECIPITATION (CHEMICAL); PROTEINS; PURIFICATION; TEMPERATURE CONTROL;

ANTIBODY-BINDING; BINDING LIGANDS; ELASTIN-LIKE PROTEIN (ELP); TEMPERATURE TRIGGER;

ANTIBODIES;

ANTIBODY; ELASTIN; HYBRID PROTEIN; IMMUNOGLOBULIN G; PROTEIN A; PROTEIN G;

ARTICLE; EXPRESSION VECTOR; HYBRIDOMA CELL CULTURE; LIGAND BINDING; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRECIPITATION; PROTEIN PURIFICATION; RABBIT; TEMPERATURE DEPENDENCE; WESTERN BLOTTING;

ANTIBODIES; BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; DNA-BINDING PROTEINS; ELASTIN; FRACTIONAL PRECIPITATION; IMMUNOGLOBULIN G; NERVE TISSUE PROTEINS; PROTEIN BINDING; TEMPERATURE;

ANIMALIA;

EID: 20344386976     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20451     Document Type: Article

References (35)

1. Akerstrom B, Bjorcks L. 1986. A physicochemical study of protein G, a molecule with unique immunoglobulin G-binding properties. J Biol Chem 261:10240-10247.
2. Bibila TA, Robinson DK. 1995. In pursuit of the optimal fed-batch process for monoclonal antibody production. Biotechnol Prog 11:1-13.
3. Bjorck L. 1988. Protein L: a novel bacterial cell wall protein with affinity for Ig L chains. J Immunol 140:1194-1197.
4. Bjorck L, Kronvall G. 1984. Purification and some properties of streptococcal protein G, a novel IgG-binding reagent. J Immunol 133: 969-974.
5. Carter P. 2001. Improving the efficacy of antibody-based cancer therapies. Nat Rev Cancer 1:118-129.
6. Chen JP, Hoffman AS. 1990. Polymer-protein conjugates. II. Affinity precipitation separation of human immunogammaglobulin by a poly(N- isopropylacrylamide)-protein A conjugate. Biomaterials 11: 631-634.
7. Dancette OP, Taboureau JL, Tournier E, Charcosset C, Blond P. 1999. Purification of immunoglobulins G by protein A/G affinity membrane chromatography. J Chromatogr B 723:61-68.
8. Ding Z, Long CJ, Hayashi Y, Bulmus EV, Hoffman AS, Stayton PS. 1999. Temperature control of biotin binding and release with a streptavidin-poly(N- isopropylacrylamide) site-specific conjugate. Bioconj Chem 10:395-400.
9. Fassina G, Ruvo M, Palombo G, Verdoliva A, Marino M. 2001. Novel ligands for the affinity-chromatographic purification of antibodies. J Biochem Biophys Methods 49:481-490.
10. Fong RB, Ding Z, Hoffman AS, Stayton PS. 2002. Affinity separation using an Fv antibody fragment-"smart" polymer conjugate. Biotechnol Bioeng 79:271-276.
11. Galaev IY, Mattiasson B. 1993. Affinity thermoprecipitation: contribution of the efficiency of ligand-protein interaction and access of the ligand. Biotechnol Bioeng 41:1101-1106.
12. Guerrier L, Flayeux I, Schwarz A, Fassina G, Boschetti E. 1998. IRIS 97: an innovative protein A-peptidomimetic solid phase medium for antibody purification. J Mol Recognit 11:107-109.
13. Gupta MN, Mattiasson B. 1994. Affinity precipitation. In: Street G, editor. Highly selective separation in biotechnology. London: Blackie Academic, p 7-33.
14. Huse K, Bohme HJ, Scholz GH. 2002. Purification of antibodies by affinity chromatography. J Biol Chem 51:217-231.
15. Kastern W, Sjobring U, Bjorck L. 1992. Structure of peptostreptococcal protein L and identification of repeat immunoglobulin light chain-binding domain. J Biol Chem 267:12820-12825.
16. Keller MA, Stiehm ER. 2000. Passive immunity in prevention and treatment of infectious diseases. Clin Microbiol Rev 13:602-614.
17. Kihlberg BM, Sjobring U, Kastern W, Bjorck L. 1992. Protein LG: a hybrid molecule with unique immunoglobulin binding properties. J Biol Chem 267:25583-25588.
18. Kostal J, Mulchandani A, Chen W. 2001. Tunable biopolymers for heavy metal removal. Macromolecules 34:2257-2261.
19. Kostal J, Mulchandani A, Chen W. 2004. Affinity purification of plasmid DNA by temperature-triggered precipitation. Biotechnol Bioeng 85: 293-297.
20. Kumar A, Kamihira M, Galaev IY, Mattiasson B, Iijima S. 2001. Type-specific separation of animal cells in aqueous two-phase systems using antibody conjugates with temperature-sensitive polymers. Biotechnol Bioeng 75:571-580.
21. Lindmark R, Thren-Tolltng K, Sjoquist J. 1983. Binding of immunoglobulins to protein a and immunoglobin levels in mammalian sera. J Immunol Methods 62:1-13.
22. Maynard JA, Maassen CBM, Leppla SH, Brasky K, Patterson JL, Iverson BL, Georgiou G. 2002. Protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity. Nat Biotechnol 20:597-601.
23. Mckinney KL, Dilwith R, Belfort G. 1995. Optimizing antibody production in batch hybridoma cell culture. J Biotechnol 40:31-48.
24. Meyer DE, Chilkoti A. 1999. Purification of recombinant proteins by fusion with thermally-responsive polypeptides. Nat Biotechnol 17: 1112-1115.
25. Meyer DE, Trabbic-Carlson K, Chilkoti A. 2001. Protein purification by fusion with an environmentally responsive elastin-like polypeptide: effect of polypeptide length on the purification of thioredoxin. Biotechnol Prog 17:720-728.
26. Moks T, Abrahmsen L, Nilsson B, Hellman U, Sjoquist J, Uhlen M. 1986. Staphylococcal protein A consists of five IgG-binding domains. Eur J Biochem 156:637-643.
27. Sambrook J, Russell DW. 2001. Molecular cloning - a laboratory manual. New York: Cold Spring Harbor.
28. Shimazu M, Mulchandani A, Chen W. 2003. Thermally triggered purification and immobilization of elastin-OPH fusions. Biotechnol Bioeng 81:74-79.
29. Stiborova H, Kostal J, Mulchandani A, Chen W. 2003. One-step metal affinity purification of histidine-tagged proteins by temperature-triggered precipitation. Biotechnol Bioeng 82:605-611.
30. Templin MF, Stoll D, Schwenk JM, Potz O, Kramer S, Joos TO. 2003. Protein microarrys: promising tools for proteomic research. Proteomics 3:2155-2166.
31. Thomas TM, Shave EE, Bate IM, Gee SC, Franklin S, Rylatt DB. 2002. Preparative electrophoresis: a general method for the purification of polyclonal antibodies. J Chromatogr A 944:161-168.
32. Trabbic-Carlson K, Meyer DE, Liu L, Piervincenzi R, Nath N, LaBean T, Chilkoti A. 2004. Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity? Protein Eng Design Select 17:57-66.
33. Urry DW. 1992. Free energy transduction in polypeptides and proteins based on inverse temperature transitions. Prog Biophys Mol Bio 57: 23-57.
34. Urry DW. 1997. Physical chemistry of biological free energy transduction demonstrated by elastic protein-based polymers. J Phys Chem B 101: 11007-11028.
35. Vandekerckhove BAE, Jones D, Punnonen J, Schols D, Lin HC, Duncan B, Bacchetta R, Vries JE, Roncarolo MG. 1993. Human Ig production and isotype switching in severe combined immunodeficient-human mice. J Immunol 151:128-137.