Recovery of a monoclonal antibody from hybridoma culture supernatant by affinity precipitation with Eudragit S-100

Bioseparation

Volumn 9, Issue 5, 2000, Pages 291-298

  Taipa, M Ângela ^a   Kaul, Rajni Hatti ^b   Mattiasson, Bo ^b   Cabrai, Joaquim M S ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b CHEMICAL CENTER   (Sweden)

Author keywords

Affinity precipitation; Eudragit S 100; Monoclonal antibodies; Purification

Indexed keywords

AFFINITY CHROMATOGRAPHY; AQUEOUS SOLUTION; DIALYSIS; ELUTION; GLYCINE; HYBRIDOMA CELL CULTURE; INCUBATION; LIGAND; MONOCLONAL ANTIBODY; PH;

AFFINITY CHROMATOGRAPHY; CELL CULTURE; DIALYSIS; PH; PRECIPITATION (CHEMICAL);

HETEROBIFUNCTIONAL LIGANDS;

MONOCLONAL ANTIBODIES;

ANTIBODIES, MONOCLONAL; HYBRIDOMAS; POLYMETHACRYLIC ACIDS; PRECIPITATION; TECHNOLOGY, PHARMACEUTICAL;

EID: 0009506003     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1011183904966     Document Type: Article

References (26)

1. Bradshaw AP and Sturgeon RJ (1990) The synthesis of soluble polymer-ligand complexes for affinity precipitation studies. Biotechnol. Tech. 4: 67-71.
2. Chen J-P (1990) Novel affinity-based processes for protein purification. J. Ferment. Bioeng. 70: 199-209.
3. Daniak MB, Izumrudov VA, Muronetz VI, Galaev I and Mattiasson B (1998-99). Affinity precipitation of monoclonal antibodies by nonstoichiometric polyelectrolyte complexes. Bioseparation 7: 231-240.
4. Galaev I and Mattiasson B (1999) 'Smart' polymers and what they could do in biotechnology and medicine. Trends Biotechnol. 17: 335-340.
5. Goding JW (1986) Monoclonal Antibodies - Principles and Practice, Academic Press, London, UK.
6. Guoqiang D, Batra R, Kaul RH, Gupta MN and Mattiasson B (1995) Alternative modes of precipitation of Eudragit S-100: apotential ligand carrier for affinity precipitation of protein. Bioseparation 5: 339-350.
7. Guoqiang D, Lali A, Kaul RH and Mattiasson B (1994) Affinity thermoprecipitation of lactate dehydrogenase and pyruvate kinase from porcine muscle using Eudragit bound Cibacron blue. J. Biotechnol. 37: 23-31.
8. Gupta MN, Kaul RH, Dissing U and Mattiasson B (1996) Affinity precipitation of proteins. J. Mol. Recognit. 9: 356-359.
9. Hill CR, Thompson LG and Kenney AC (1989) Immunopurification. In: Harris ELV & Angal S (eds.) Protein Purification Methods - A Practical Approach, (pp. 282-292) IRL Press, Oxford, UK.
10. Irwin JA and Tipton KF (1995) Affinity precipitation: a novel approach to protein purification. Essays Biochem. 29: 137-157.
11. Kamihira M, Iijima S and Kobayashi T (1992a) Stabilities of antigen and antibody under elution conditions in immunoaffinity chromatography using monoclonal antibody. Bioseparation 3: 185-188.
12. Kamihira M, Kaul R and Mattiasson B (1992b) Purification of recombinant protein A by aqueous two-phase extraction and affinity precipitation for the purification of lactate dehydrogenase. Biotechnol. Bioeng. 40: 1381-1387.
13. Kaul RH and Mattiasson B (1992) Secondary purification. Bioseparation 3: 1-26.
14. Kondo A, Kaneko T and Higashitani K (1994) Development and application of thermo-sensitive immunomicrospheres for antibody purification. Biotechnol. Bioeng. 44: 1-6.
15. Linné-Larsson E and Mattiasson B (1994) Isolation of concanavalin A by affinity precipitation. Biotechnol. Tech. 8: 51-56.
16. Linné-Larsson E, Galaev IY, Lindahl L and Mattiasson B (1996) Affinity precipitation of Concanavalin A with p-aminophenyl-α-D-glucopyranoside modified Eudragit S-100. Bioseparation 6: 273-282.
17. Lowe CR, Burton SJ, Burton NP, Alderton WK, Pitts JM and Thomas JA (1992) Designer dyes. Biomimetic ligands for the purification of pharmaceutical proteins by affinity chromatography. Trends Biotechnol. 10, 442-448.
18. Mattiasson B, Kumar A and Galaev I (1998) Affinity precipitation of proteins: design criteria for an efficient polymer. J. Mol. Recognit. 11: 211-216.
19. Powers D, Carbonell R and Kilpatrick PK (1994) Affinity precipitation of antibody by ligand-modified phospholipids. Biotechnol. Bioeng. 44: 509-522.
20. Sadana A and Beelaram AM (1994) Protein inactivations during novel bioseparation techniques. Bioseparation 4: 39-61.
21. Shu H-C, Guoqiang D, Kaul RH and Mattiasson B (1994) Purification of the D-lactate dehydrogenase from Leuconostoc mesenteroides ssp. cremoris using a sequential precipitation procedure. J. Biotechnol. 34: 1-11.
22. Taipa MA, Kaul RH, Mattiasson B and Cabral JMS (1998) Preliminary studies on the purification of a monoclonal antibody by affinity precipitation. J. Mol. Recognit. 11: 240-242.
23. Taipa MA, Liebeton K, Costa JV, Cabral JMS and Jaeger K-E (1995) Lipase from Chromobacterium viscosum: biochemical characterization indicating homology to lipase from Pseudomonas glumae. Biochim. Biophys. Acta 1256: 396-402.
24. Taniguchi M, Kobayashi M and Fujii M (1989a) Properties of a reversible soluble-insoluble cellulase and its application to repeated hydrolysis of crystaline cellulose. Biotechnol. Bioeng. 34: 1092-1097.
25. Taniguchi M, Kobayashi M, Natsui K and Fujii M (1989b) Purification of staphylococcal protein A by affinity precipitation using a reversibly insoluble polymer with human IgG as a ligand. J. Ferment. Bioeng. 68: 32-36.
26. Yarmush ML, Antonsen KP, Sundaram S and Yarmush DM (1992) Immunoadsorption: strategies for antigen elution and production of reusable adsorbents. Biotechnol. Prog. 8: 168-176.