Affinity separation using an Fv antibody fragment-"smart" polymer conjugate

Biotechnology and Bioengineering

Volumn 79, Issue 3, 2002, Pages 271-276

  Fong, Robin B ^a   Ding, Zhongli ^a   Hoffman, Allan S ^a   Stayton, Patrick S ^a  

^a University of Washington   (United States)

Author keywords

LCST; Lysozyme; Precipitation; Temperature responsive

Indexed keywords

ANTIBODIES; ANTIGENS; DISSOLUTION; ESCHERICHIA COLI; GENETIC ENGINEERING; ORGANIC POLYMERS; PRECIPITATION (CHEMICAL); PURIFICATION; SEPARATION;

CRITICAL TEMPERATURE BEHAVIOR;

BIOTECHNOLOGY;

ANTIBODY CONJUGATE; DIETHYLAMINE; EGG WHITE; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN G ANTIBODY; LYSOZYME; POLY(N ISOPROPYLACRYLAMIDE); POLYMER; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; WATER;

ANTIBODY PRODUCTION; ANTIGEN BINDING; ANTIGENICITY; ARTICLE; BINDING AFFINITY; COOLING; DISSOLUTION; ESCHERICHIA COLI; GENETIC ENGINEERING; HEATING; PRECIPITATION; TEMPERATURE DEPENDENCE; THERMAL ANALYSIS;

ACRYLIC RESINS; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CHICKENS; CHROMATOGRAPHY, AFFINITY; FLUOROIMMUNOASSAY; HUMANS; IMMUNOGLOBULIN FRAGMENTS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MURAMIDASE; PRECIPITATION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TEMPERATURE;

ESCHERICHIA COLI;

EID: 0037026239     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10315     Document Type: Article

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