Relative quantification and detection of different types of infectious bursal disease virus in bursa of Fabricius and cloacal swabs using real time RT-PCR SYBR green technology

Research in Veterinary Science

Volumn 82, Issue 1, 2007, Pages 126-133

  Li, Y P ^a,b   Handberg, K J ^a   Kabell, S ^a   Kusk, M ^a   Zhang, M F ^b   Jorgensen P H ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Bursa of Fabricius (BF); Infectious bursal disease virus (IBDV); Quantification; Quantitative real time RT PCR

Indexed keywords

BETA ACTIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; RNA 28S;

ANIMAL TISSUE; ARTICLE; BURSA FABRICII; CHICKEN; CLOACA; CONTROLLED STUDY; INFECTIOUS BURSAL DISEASE VIRUS; INOCULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS INFECTION; VIRUS LOAD; VIRUS STRAIN; VIRUS VIRULENCE;

ACTINS; ANIMALS; BURSA OF FABRICIUS; CHICKENS; CLOACA; FLUORESCENT DYES; GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (NADP+); INFECTIOUS BURSAL DISEASE VIRUS; POULTRY DISEASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 28S; SPECIFIC PATHOGEN-FREE ORGANISMS;

GALLUS GALLUS; INFECTIOUS BURSAL DISEASE VIRUS;

EID: 33845239195     PISSN: 00345288     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.rvsc.2006.03.002     Document Type: Article

References (30)

1. Bismar T.A., Bianco F.J., Zhang H., Li X., Sarkar F.H., Sakr W.A., Grignon D.J., and Che M. Quantification of G250 mRNA expression in renal epithelial neoplasms by real-time reverse transcription-PCR of dissected tissue from paraffin sections. Pathology 35 (2003) 513-517
2. Boot H.J., ter Huurne A.A., Hoekman A.J., Peeters B.P., and Gielkens A.L. Rescue of very virulent and mosaic infectious bursal disease virus from cloned cDNA: VP2 is not the sole determinant of the very virulent phenotype. J. Virol. 74 (2000) 6701-6711
3. Bustin S.A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25 (2000) 169-193
4. Cao Y.C., Yeung W.S., Law M., Bi Y.Z., Leung F.C., and Lim B.L. Molecular characterization of seven Chinese isolates of infectious bursal disease virus: classical, very virulent, and variant strains. Avian Dis. 42 (1998) 340-351
5. Clementi M., Menzo S., Manzin A., and Bagnarelli P. Quantitative molecular methods in virology. Arch. Virol. 140 (1995) 1523-1539
6. Cursiefen D., Kaufer I., and Becht H. Loss of virulence in a small plaque mutant of the infectious bursal disease virus. Arch. Virol. 59 (1979) 39-46
7. Ginzinger D.G. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp. Hematol. 30 (2002) 503-512
8. Gorski D.H., Leal A.D., and Goydos J.S. Differential expression of vascular endothelial growth factor-A isoforms at different stages of melanoma progression. J. Am. Coll. Surg. 197 (2003) 408-418
9. Hassan M.K., al Natour M.Q., Ward L.A., and Saif Y.M. Pathogenicity, attenuation, and immunogenicity of infectious bursal disease virus. Avian Dis. 40 (1996) 567-571
10. Jackwood D.J., and Sommer S.E. Identification of infectious bursal disease virus quasispecies in commercial vaccines and field isolates of this double-stranded RNA virus. Virology 304 (2002) 105-113
11. Jackwood D.J., Spalding B.D., and Sommer S.E. Real-time reverse transcriptase-polymerase chain reaction detection and analysis of nucleotide sequences coding for a neutralizing epitope on infectious bursal disease viruses. Avian Dis. 47 (2003) 738-744
12. Kibenge F.S., Dhillon A.S., and Russell R.G. Biochemistry and immunology of infectious bursal disease virus. J. Gen. Virol. 69 (1988) 1757-1775
13. Lange H., Muller H., Kaufer I., and Becht H. Pathogenic and structural properties of wild type infectious bursal disease virus (IBDV) and virus grown in vitro. Arch. Virol. 92 (1987) 187-196
14. Li Y.P., Bang D.D., Handberg K.J., Jorgensen P.H., and Zhang M.F. Evaluation of the suitability of six host genes as internal control in real time RT-PCR assays in chicken embryo fibroblasts infected with infectious bursal disease virus. Vet. Microbiol. 110 (2005) 155-165
15. Lim B.L., Cao Y., Yu T., and Mo C.W. Adaptation of very virulent infectious bursal disease virus to chicken embryonic fibroblasts by site-directed mutagenesis of residues 279 and 284 of viral coat protein VP2. J. Virol. 73 (1999) 2854-2862
16. Mackay I.M., Arden K.E., and Nitsche A. Real-time PCR in virology. Nucleic Acids Res. 30 (2002) 1292-1305
17. Mill J., Asherson P., Browes C., D'Souza U., and Craig I. Expression of the dopamine transporter gene is regulated by the 3' UTR VNTR: evidence from brain and lymphocytes using quantitative RT-PCR. Am. J. Med. Genet. 114 (2002) 975-979
18. Moody A., Sellers S., and Bumstead N. Measuring infectious bursal disease virus RNA in blood by multiplex real-time quantitative RT-PCR. J. Virol. Methods 85 (2000) 55-64
19. Mundt E. Tissue culture infectivity of different strains of infectious bursal disease virus is determined by distinct amino acids in VP2. J. Gen. Virol. 80 (1999) 2067-2076
20. Peters M.A., Lin T.L., and Wu C.C. Infectious bursal disease virus polyprotein expression arrests growth and mitogenic stimulation of B lymphocytes. Arch. Virol. 149 (2004) 2413-2426
21. Raue R., and Muller H. Detection and quantification of IBDV by real-time RT-PCR. II. International symposium on infectious Bursal disease and chicken infectious anaemia (2001), Rauischholzhausen, Germany 271-277
22. Rautenschlein S., Yeh H.Y., and Sharma J.M. Comparative immunopathogenesis of mild, intermediate, and virulent strains of classic infectious bursal disease virus. Avian Dis. 47 (2003) 66-78
23. Sasaki H., Fukai I., Kiriyama M., Kaji M., Yano M., Yamakawa Y., and Fujii Y. Thymidylate synthase and dihydropyrimidine dehydrogenase mRNA levels in thymoma. Surg. Today 33 (2003) 83-88
24. Tanimura N., Tsukamoto K., Nakamura K., Narita M., and Maeda M. Association between pathogenicity of infectious bursal disease virus and viral antigen distribution detected by immunohistochemistry. Avian Dis. 39 (1995) 9-20
25. Tsukamoto K., Tanimura N., Mase M., and Imai K. Comparison of virus replication efficiency in lymphoid tissues among three infectious bursal disease virus strains. Avian Dis. 39 (1995) 844-852
26. van den Berg T.G., Gonze M., and Meulemans G. Acute infectious bursal disease in poultry: isolation and characterization of a highly virulent strain. Avian Pathol. 20 (1991) 133-143
27. van den Berg T.P., Eterradossi N., Toquin D., and Meulemans G. Infectious bursal disease (Gumboro disease). Rev. Sci. Tech. 19 (2000) 509-543
28. van Loon A.A., de Haas N., Zeyda I., and Mundt E. Alteration of amino acids in VP2 of very virulent infectious bursal disease virus results in tissue culture adaptation and attenuation in chickens. J. Gen. Virol. 83 (2002) 121-129
29. Yamaguchi T., Kondo T., Inoshima Y., Ogawa M., Miyoshi M., Yanai T., Masegi T., Fukushi H., and Hirai K. In vitro attenuation of highly virulent infectious bursal disease virus: some characteristics of attenuated strains. Avian Dis. 40 (1996) 501-509
30. Zhang M.F., Huang G.M., and Qiao S. Early stages of infectious bursal disease virus infection in chickens detected by in situ reverse transcriptase-polymerase chain reaction. Avian Pathol. 31 (2002) 593-597