Measuring infectious bursal disease virus RNA in blood by multiplex real-time quantitative RT-PCR

Journal of Virological Methods

Volumn 85, Issue 1-2, 2000, Pages 55-64

  Moody, Adrian ^a   Sellers, Scott ^a   Bumstead, Nat ^a  

^a INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

Author keywords

Blood; IBDV; Multiplex; PCR; Reverse transcription; TaqMan

Indexed keywords

VIRUS RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD ANALYSIS; CHICKEN; CONTROLLED STUDY; MORTALITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REGRESSION ANALYSIS; REOVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARDIZATION; VIRUS INFECTION; VIRUS LOAD;

ANIMALS; BIRNAVIRIDAE INFECTIONS; BURSA OF FABRICIUS; CHICKENS; INFECTIOUS BURSAL DISEASE VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL;

GALLUS GALLUS; INFECTIOUS BURSAL DISEASE VIRUS;

EID: 0033973247     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(99)00156-1     Document Type: Article

References (19)

1. Bayliss C.D., Spies U., Shaw K., Peters R.W., Papageorgiou A., Muller H., Boursnell M.E.G. A comparison of the sequence of segment A of four infectious bursal disease virus strains and identification of a variable region in the VP2. J. Gen. Vir. 71:1990;1303-1312.
2. Bumstead N., Reece R.L., Cook J.K.A. Genetic differences in susceptibility of chicken lines to infection with Infectious Bursal Disease Virus. Poult. Sci. 72:1993;403-410.
3. Bygrave A.C., Faragher J.T. Mortality associated with Gumbro disease. Vet. Rec. 86:1970;758-759.
4. Clementi M., Menzo S., Manzin A., Bagnarelli P. Quantitative molecular methods in virology. Arch. Vir. 140:1995;1523-1539.
5. Desjardin L.E., Chen Y., Perkins M.D., Teixeira L., Cave M.D., Eisenach K.D. Comparison of the ABI 7700 System (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of Tuberculosis. J. Clin. Microbiol. 36(7):1998;1964-1968.
6. Freeman W.M., Walker S.J., Vrana K.E. Quantitative RT-PCR: Pitfalls and potential. BioTechniques. 26:1999;112-125.
7. Gibson U.E.M., Heid C.A., Williams P.M. A novel method for real time quantitative RT-PCR. Genome Res. 6:1996;995-1001.
8. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitative PCR. Genome Res. 6:1996;986-994.
9. Henry C.W., Brewer R.N., Edgar S.A. Studies on Infectious Bursal Disease in Chickens. 2. Scoring microscopic lesions in the bursa of Fabricius, thymus, spleen, and kidney in gnotobiotic and battery reared white leghorns experimentally infected with infectious bursal disease virus. Poult. Sci. 59:1980;1006-1017.
10. Jackwood D.J., Nielsen C.K. Detection of infectious bursal disease viruses in commercially reared chickens using the reverse transcriptase/polymerase chain reaction-restriction endonuclease assay. Avian Dis. 41:1997;137-143.
11. Kibenge F.S.B., Dhillon A.S., Russell R.G. Biochemistry and immunology of infectious bursal disease virus. J. Gen. Virol. 69:1988;1757-1775.
12. Lee L.H., Ting L.J., Shien J.H., Shien H.K. Single tube, noninterrupted reverse transcription-PCR for detection of infectious bursal disease virus. J. Clin. Microbiol. 32(5):1994;1268-1272.
13. PE Applied Biosystems User Bulletin #5: http://www2.perkin-elmer.com/ab.
14. Qian B., Kibenge F.S.B. Observations on polymerase chain reaction amplification of infectious bursal disease virus dsRNA. J. Virol. Methods. 47:1994;237-242.
15. Souaze F., Ntodou-Thome A., Tran C.Y., Rostene W., Forgez P. Quantitative RT-PCR: Limits and Accuracy. BioTechniques. 21:1996;280-285.
16. Stram Y., Meir R., Molad T., Blumenkranz R., Malkinson M., Weisman Y. Applications of the polymerase chain reaction to detect infectious bursal disease virus in naturally infected chickens. Avian Dis. 38:1994;879-884.
17. Tham K.M., Young L.W., Moon C.D. Detection of infectious bursal disease virus by reverse transcription-polymerase chain reaction amplification of the virus segment A gene. J. Virol. Methods. 53:1995;201-212.
18. Wu C.C., Lin T.L., Zhang H.G., Davis V.S., Boyle J.A. Molecular detection of infectious bursal disease virus by polymerase chain reaction. Avian Dis. 36:1992;221-226.
19. Wu C.C., Lin T.L., Akin A. Quantitative competitive polymerase chain reaction for detection and quantification of infectious bursal disease virus cDNA and RNA. J. Virol. Methods. 66:1997;29-38.