Long-term suppression of telomerase expression in HeLa cell clones, transfected with an expression vector carrying siRNA targeting hTERT mRNA

International Journal of Oncology

Volumn 29, Issue 1, 2006, Pages 279-288

  Kurvinen, Kaisa ^a   Syrjänen, Stina ^a   Johansson, Bo ^b  

^a UNIVERSITY OF TURKU   (Finland)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

hTERT; mRNA; siRNA; Telomerase

Indexed keywords

MESSENGER RNA; SMALL INTERFERING RNA; TELOMERASE; TERT PROTEIN, HUMAN;

APOPTOSIS; ARTICLE; CELL CLONE; CELL PROLIFERATION; CELL SHAPE; COMPARATIVE STUDY; ENZYMOLOGY; FEMALE; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HELA CELL; HUMAN; METABOLISM; PATHOLOGY; RNA INTERFERENCE; TELOMERE; TIME; UTERINE CERVIX TUMOR;

APOPTOSIS; CELL PROLIFERATION; CELL SHAPE; CLONE CELLS; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; GENETIC VECTORS; HELA CELLS; HUMANS; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING; TELOMERASE; TELOMERE; TIME FACTORS; TRANSFECTION; UTERINE CERVICAL NEOPLASMS;

EID: 33750990682     PISSN: 10196439     EISSN: 17912423     Source Type: Journal    
DOI: 10.3892/ijo.29.1.279     Document Type: Article

References (35)

1. Blackburn EH: Telomere states and cell fates. Nature 408: 53-56, 2000.
2. Blackburn EH: Telomeres and telomerase: their mechanisms of action and the effects of altering their functions. FEBS Lett 579: 859-862, 2005.
3. Huffman KE, Levene SD, Tesmer VM, Shay JW and Wright WE: Telomere shortening is proportional to the size of the G-rich telomeric 3'-overhang. J Biol Chem 275: 19719-19722, 2000.
4. Dong CK, Masutomi K and Hahn WC: Telomerase: regulation, function and transformation. Crit Rev Oncol Hematol 54: 85-93, 2005.
5. Janknecht R: On the road to immortality: hTERT upregulation in cancer cells. FEBS Lett 564: 9-13, 2004.
6. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, Coviello GM, Wright WE, Weinrich SL and Shay JW: Specific association of human telomerase activity with immortal cells and cancer. Science 266: 2011-2015, 1994.
7. Wright WE, Piatyszek MA, Rainey WE, Byrd W and Shay JW: Telomerase activity in human germline and embryonic tissues and cells. Dev Genet 18: 173-179, 1996.
8. Hahn WC, Stewart SA, Brooks MW, York SG, Eaton E, Kurachi A, Beijersbergen RL, Knoll JH, Meyerson M and Weinberg RA: Inhibition of telomerase limits the growth of human cancer cells. Nat Med 5: 1164-1170, 1999.
9. Zhang X, Mar V, Zhou W, Harrington L and Robinson MO: Telomere shortening and apoptosis in telomerase-inhibited human tumor cells. Genes Dev 13: 2388-2399, 1999.
10. Saretzki G, Ludwig A, von Zglinicki T and Runnebaum IB: Ribozyme-mediated telomerase inhibition induces immediate cell loss but not telomere shortening in ovarian cancer cells. Cancer Gene Ther 8: 827-834, 2001. (Pubitemid 32983139)
11. Pascolo E, Wenz C, Lingner J, Hauel N, Priepke H, Kauffmann I, Garin-Chesa P, Rettig WJ, Damm K and Schnapp A: Mechanism of human telomerase inhibition by BIBR1532, a synthetic, non-nucleosidic drug candidate. J Biol Chem 277: 15566-15572, 2002.
12. Kraemer K, Fuessel S, Schmidt U, Kotzsch M, Schwenzer B, Wirth MP and Meye A: Antisense-mediated hTERT inhibition specifically reduces the growth of human bladder cancer cells. Clin Cancer Res 9: 3794-3800, 2003.
13. Caplen NJ: RNAi as a gene therapy approach. Expert Opin Biol Ther 3: 575-586, 2003.
14. Kosciolek BA, Kalantidis K, Tabler M and Rowley PT: Inhibition of telomerase activity in human cancer cells by RNA interference. Mol Cancer Ther 2: 209-216, 2003.
15. Shammas MA, Koley H, Batchu RB, Bertheau RC, Protopopov A, Munshi NC and Goyal RK: Telomerase inhibition by siRNA causes senescence and apoptosis in Barrett's adenocarcinoma cells: mechanism and therapeutic potential. Mol Cancer 4: 24, 2005.
16. Xia Y, Lin RX, Zheng SJ, Yang Y, Bo XC, Zhu DY and Wang SQ: Effective siRNA targets screening for human telomerase reverse transcriptase. World J Gastroenterol 11: 2497-2501, 2005.
17. Bryan TM, Englezou A, Dunham MA and Reddel RR: Telomere length dynamics in telomerase-positive immortal human cell populations. Exp Cell Res 239: 370-378, 1998. (Pubitemid 28366637)
18. Wege H, Chui MS, Le HT, Tran JM and Zern MA: SYBR Green real-time telomeric repeat amplification protocol for the rapid quantification of telomerase activity. Nucleic Acids Res 31: e3, 2003.
19. Miller SA, Dykes DD and Polesky HF: A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16: 1215, 1988.
20. Elbashir SM, Harborth J, Weber K and Tuschl T: Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods 26: 199-213, 2002.
21. Xu D, Gruber A, Björkholm M, Peterson C and Pisa P: Suppression of telomerase reverse transcriptase (hTERT) expression in differentiated HL-60 cells: regulatory mechanisms. Br J Cancer 80: 1156-1161, 1999.
22. Holt SE, Aisner DL, Shay JW and Wright WE: Lack of cell cycle regulation of telomerase activity in human cells. Proc Natl Acad Sci USA 94: 10687-10692, 1997.
23. Yi X, Shay JW and Wright WE: Quantitation of telomerase components and hTERT mRNA splicing patterns in immortal human cells. Nucleic Acids Res 29: 4818-4825, 2001.
24. Saretzki G, Petersen S, Petersen I, Kölble K and von Zglinicki T: hTERT gene dosage correlates with telomerase activity in human lung cancer cell lines. Cancer Lett 176: 81-91, 2002.
25. Swiggers SJJ, Nibbeling HAJ, Zeilemaker A, Kuijpers MA, Mattern KA and Zijlmans JMJM: Telomerase activity level, but not hTERT mRNA and hTR level, regulates telomere length in telomerase-reconstituted primary fibroblasts. Exp Cell Res 297: 434-443, 2004. (Pubitemid 38798405)
26. Smogorzewska A, van Steensel B, Bianchi A, Oelmann S, Schaefer MR, Schnapp G and de Lange T: Control of human telomere length by TRF1 and TRF2. Mol Cell Biol 20: 1659-1668, 2000. (Pubitemid 30100185)
27. Rodier F, Kim SH, Nijjar T, Yaswen P and Campisi J: Cancer and aging: the importance of telomeres in genome maintenance. Int J Biochem Cell Biol 37: 977-990, 2005.
28. Yaswen P and Stampfer MR: Molecular changes accompanying senescence and immortalization of cultured human mammary epithelial cells. Int J Biochem Cell Biol 34: 1382-1394, 2002.
29. Scherr M, Morgan MA and Eder M: Gene silencing mediated by small interfering RNAs in mammalian cells. Curr Med Chem 10: 245-256, 2003.
30. Natarajan S, Chen Z, Wancewicz EV, Monia BP and Corey DR: Telomerase reverse transcriptase (hTERT) mRNA and telomerase RNA (hTR) as targets for downregulation of telomerase activity. Oligonucleotides 14: 263-273, 2004.
31. Masutomi K, Yu EY, Khurts S, Ben-Porath I, Currier JL, Metz GB, Brooks MW, Kaneko S, Murakami S, De Caprio JA, Weinberg RA, Stewart SA and Hahn WC: Telomerase maintains telomere structure in normal human cells. Cell 114: 241-253, 2003.
32. Guo Y, Liu J, Li YH, Song TB, Wu J, Zheng CX and Xue CF: Effect of vector-expressed shRNAs on hTERT expression. World J Gastroenterol 11: 2912-2915, 2005. (Pubitemid 40790059)
33. Nakamura M, Masutomi K, Kyo S, Hashimoto M, Maida Y, Kanaya T, Tanaka M, Hahn WC and Inoue M: Efficient inhibition of human telomerase reverse transcriptase expression by RNA interference sensitizes cancer cells to ionizing radiation and chemotherapy. Hum Gene Ther 16: 859-868, 2005.
34. Ludwig A, Saretzki G, Holm PS, Tiemann F, Lorenz M, Emrich T, Harley CB and von Zglinicki T: Ribozyme cleavage of telomerase mRNA sensitizes breast epithelial cells to inhibitors of topoisomerase. Cancer Res 61: 3053-3061, 2001.
35. Yuan Z and Mei HD: Inhibition of telomerase activity with hTERT antisense increases the effect of CDDP-induced apoptosis in myeloid leukemia. Hematol J 3: 201-205, 2002.