Telomerase inhibition by siRNA causes senescence and apoptosis in Barrett's adenocarcinoma cells: Mechanism and therapeutic potential

Molecular Cancer

Volumn 4, Issue , 2005, Pages

  Shammas, Masood A ^a,b,d   Koley, Hemanta ^a,b,d   Batchu, Ramesh B ^e   Bertheau, Robert C ^e   Protopopov, Alexei ^b   Munshi, Nikhil C ^a,b,d   Goyal, Raj K ^a,c,d,e  

^a VA BOSTON HEALTHCARE SYSTEM   (United States)

^b DANA FARBER CANCER INSTITUTE   (United States)

^c BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

^d HARVARD MEDICAL SCHOOL   (United States)

^e Department of Research and Development ^*

Author keywords

Apoptosis; Barrett's esophageal adenocarcinoma; Cancer prevention; Cancer treatment; P63; P73; Senescence; SiRNA; Telomerase; Telomere

Indexed keywords

CASPASE 8; FAS ANTIGEN; FAS LIGAND; GROWTH ARREST AND DNA DAMAGE INDUCIBLE PROTEIN 45; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; PROTEIN BCL 10; PROTEIN P16; PROTEIN P53; PROTEIN P63; PROTEIN P73; SMALL INTERFERING RNA; TELOMERASE; TRANSCRIPTION FACTOR E2F1;

APOPTOSIS; ARTICLE; BARRETT ESOPHAGUS; CARCINOMA CELL; CELL CYCLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ESOPHAGEAL ADENOCARCINOMA; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PROTEIN INTERACTION; SENESCENCE; TELOMERE; UPREGULATION; WESTERN BLOTTING; ADENOCARCINOMA; CELL AGING; CELL LINE; CELL PROLIFERATION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PATHOLOGY; PHYSIOLOGY;

ADENOCARCINOMA; APOPTOSIS; CELL AGING; CELL LINE; CELL PROLIFERATION; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, NEOPLASTIC; RNA, SMALL INTERFERING; TELOMERASE; TELOMERE;

EID: 26844458316     PISSN: 14764598     EISSN: 14764598     Source Type: Journal    
DOI: 10.1186/1476-4598-4-24     Document Type: Article

References (30)

1. Schmitt CA: Senescence, apoptosis and therapy - cutting the lifelines of cancer. Nat Rev Cancer 2003, 3(4):286-295.
2. Blackburn EH: Telomerases. Ann Rev Biochem 1992, 61:113-129.
3. Shay JW, Wright WE: Senescence and immortalization: role of telomeres and telomerase. Carcinogenesis 2004, 10:1093.
4. Tominaga K, Olgun A, Smith JR, Pereira-Smith OM: Genetics of cellular senescence. Mech of Ageing & Dev 2002, 123 (8): 927-936.
5. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, Coviello GM, Wright WE, Weinrich SL, Shay JW: Specific association of human telomerase activity with immortal cells and cancer. Science 1994, 266(5193): 2011-2015.
6. Herbert BS, Pongracz K, Shay JW, Gryaznov SM, Shea-Herbert B: Oligonucleotide N3′-->P5′ phosphoramidates as efficient telomerase inhibitors. Oncogene 2002, 21(4):638-42. Erratum in: Oncogene 2002, 21(17):2774.
7. Shammas, MA, Koley, H, Beer DG, Li C, Goyal RK and Munshi NC: Growth arrest, apoptosis and telomere shortening of Barrett's associated adenocarcinoma cells by a telomerase inhibitor. Gastroenterology 2004, 126(5):1337-46.
8. Incles CM, Schultes CM, Kempski H, Koehler H, Kelland LR, Neidles S: A G-quadruplex telomere targeting agent produces p16-associated senescence and chromosomal fusions in human prostate cancer cells. Mol Cancer Ther 2004, 3(10): 1201-1206.
9. Stiewe T: p73alpha in apoptosis. Apoptosis 2001, 6;447-452.
10. Gong JG, Costanzo A, Yang HQ, Melino G, Kaelin WG, Jr, Levrero M, wang JY: The tyrosine kinase c-abl regulates p73 in apoptotic response to cisplatin-induced DNA damage. Nature 1999, 399(6738): 806-809.
11. Shammas, MA, Simmons, CG, Corey, DR, and Shmookler Reis, RJ: Telomerase inhibition by peptide nucleic acids reverses 'immortality' of transformed human cells. Oncogene 1999, 18: 6191-200.
12. Barma DK, Elayadi A Falck JR, Corey DR: Inhibition of telomerase by BIBR1532 and related analogues. Bioorg Med Chem Lett 2003, 13(7): 1333-1336.
13. + T lymphocytes. Clin Cancer Res 2004, 10(3): 828-839.
14. Spechler SJ, Goyal RK: Barrett's esophagus. N Engl J Med 1986, 315(6):362-71.
15. Devesa SS, Blot WJ, Fraumeni JF Jr: Changing patterns in the incidence of esophageal and gastric carcinoma in the United States. Cancer 1998, 83(10):2049-53.
16. Lord RV, Salonga D, Danenberg KD, Peters JH, DeMeester TR, Park JM, Johansson J, Skinner KA, Chandrasoma P, DeMeester SR, Bremner CG, Tsai PI, Danenberg PV: Telomerase reverse transcriptase expression is increased early in the Barrett's metaplasia, dysplasia, adenocarcinoma sequence. J Gastrointest Surg 2000, 4(2):135-42.
17. Shammas, MA, Shmookler Reis, RJ, Akiyama, M, Koley, H, Chauhan, D, Hideshima, T, Goyal, RK, Hurley, LH, Anderson, KC, and Munshi, NC: Telomerase Inhibition and Cell Growth Arrest by G-Quadruplex Interactive Agent in Multiple Myeloma. Mol Cancer Ther 2003, 2(9):825-833.
18. Song E, Lee SK, Wang J, Ince N, Ouyang N, Min J, Chen J, Shankar P, Lieberman J: RNA interference targeting Fas protects mice from fulminant hepatitis. Nat Med 2003, 9(3):347-351.
19. Harley, C. B., Futcher, A. B., and Greider, C. W: Telomeres shorten during aging of human fibroblasts. Nature 1990, 345: 458-60, 1990.
20. Shammas, M. A. and Shmookler Reis, R. J: Recombination and its roles in DNA repair, cellular immortalization and cancer. Age 1999, 22: 71-88.
21. Shay JW, Roninson IB: Hallmarks of senescence in carcinogenesis and cancer therapy. Oncogene 2004, 12;23(16):2919-2933.
22. Murphy JF, McGregor JL, Leung LL: Senescent human neutrophil binding to thrombospondin (TSP): evidence for a TSP-independent pathway of phagocytosis by macrophages. Br J Haematol 1998, 102(4):957-964.
23. Volker, M.; Mone, M. J.; Karmakar, P.; van Hoffen, A.; Schul, W.; Vermeulen, W.; Hoeijmakers, J. H. J.; van Driel, R.; van Zeeland, A. A.; Mullenders, L. H. F: Sequential assembly of the nucleotide excision repair factors in vivo. Molec. Cell 2001, 8: 213-224.
24. Bertin J, Guo Y, Wang L, Srinivasula SM, Jacobson MD, Poyet JL, Merriam S, Du MQ, Dyer MJ, Robison KE, DiStefane PS, Alnemri ES: Card9 is a novel caspase recruitment domain-containing protein that interacts with BCL10/CLAP and activates NF-kappa B. J. Biol. Chem 2000, 275(52): 41082-41086.
25. Harborth J, Elbashir SM, Bechert K, Tuschl T, Weber K: Identification of essential genes in cultured mammalian cells using small interfering RNAs. J Cell Sci 2001, 114, 4557-4565.
26. Shammas, M. A., Raney, K. D., Subramanian, S., and Shmookler Reis, R. J: Telomere length, cell growth potential, and DNA integrity of human immortal cells are all compromised by peptide nucleic acids targeted to the telomere or telomerase. Experimental Cell Research 2004, 295(1):204-214.
27. Lansdorp PM, Verwoerd NP, van de Rijke FM, Dragowska V, Little MT, Dirks RW, Raap AK, Tanke HJ: Heterogeneity in telomere length of human chromosomes. Hum Mol Genet 1996, 5(5):685-691.
28. Dimri G, Lee P, Basile X, Acosta G, Scott M, and Roskelley G et al: A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc. Natl. Acad. Sci. USA 1995, 92:9363-9367.
29. Li, C. and Hung Wong, W: Model-based analysis of oligonucleotide arrays: model validation, design issues and standard error application. Genome Biol 2001, 2.
30. Li, C. and Wong, W. H: Model-based analysis of oligonucleotide arrays: expression index computation and outlier detection. Proc Natl Acad Sci USA 2001, 98: 31-36.