Sensitivity of P-glycoprotein tryptophan residues to benzodiazepines and ATP interaction

Biophysical Chemistry

Volumn 125, Issue 1, 2007, Pages 143-150

  Lima, Sofia A C ^a   Cordeiro da Silva, Anabela ^b   de Castro, Baltazar ^a   Gameiro, Paula ^a  

^a Campus Universitário de Santiago   (Portugal)

^b UNIVERSITY OF PORTO   (Portugal)

Author keywords

ATP; Benzodiazepines; Interactions; Intrinsic fluorescence; P glycoprotein

Indexed keywords

ADENOSINE TRIPHOSPHATE; BENZODIAZEPINE DERIVATIVE; BROMAZEPAM; CHLORDIAZEPOXIDE; FLURAZEPAM; GLYCOPROTEIN P; IMIDAZOLE; PROTEIN; TRYPTOPHAN;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; FLAME PHOTOMETRY; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; ANIMALS; BINDING SITES; BROMAZEPAM; CATALYTIC DOMAIN; CHLORDIAZEPOXIDE; FLUORESCENCE; FLURAZEPAM; MICE; MODELS, CHEMICAL; P-GLYCOPROTEIN; PICHIA; TRYPTOPHAN;

EID: 33750698584     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bpc.2006.07.006     Document Type: Article

References (41)

1. Schinkel A.H. P-glycoprotein, a gatekeeper in the blood-brain barrier. Adv. Drug Deliv. Rev. 36 (1999) 179-194
2. Sharom F.J. The P-glycoprotein efflux pump: how does it transport drugs?. J. Membr. Biol. 160 (1997) 161-175
3. Germann U.A. P-glycoprotein-a mediator of multidrug resistance in tumour cells. Eur. J. Cancer Res. 32A (1996) 927-944
4. August B.J. P-glycoprotein, secretory transport, and other barriers to the oral delivery of anti-HIV drugs. Adv. Drug Deliv. Rev 39 (1999) 105-116
5. Schutz H. Benzodiazepines: A Handbook (1982), Springer-Verlag, Heldelberg
6. Aquilante C.L., Letrent S.P., Pollack G.M., and Brouwer K.L.R. Increased brain P-glycoprotein in morphine tolerant rats. Life Sci. 66 (2000) Pl47-Pl51
7. Letrent S.P., Pollack G.M., Brouwer K.R., and Brouwer K.L.R. Effects of a potent and specific P-glycoprotein inhibitor on the blood-brain barrier distribution and antinociceptive effect of morphine in the rat. Drug Metab. Dispos. 27 (1999) 827-834
8. Rosenberg M.F., Callaghan R., Ford R.C., and Higgins C.F. Structure of the multidrug resistance P-glycoprotein to 2.5 nm resolution determined by electron microscopy and image analysis. J. Biol. Chem. 272 (1997) 10685-10694
9. Higgins C.F. ABC transporters: physiology, structure and mechanism-an overview. Res. Microbiol. 152 (2001) 205-210
10. Sharom F.J., Liu R.H., and Romsicki Y. Spectroscopic and biophysical approaches for studying the structure and function of the P-glycoprotein multidrug transporter. Biochem. Cell Biol. -Biochim. Biol. Cell. 76 (1998) 695-708
11. Sharom F.J., Liu R.H., Romsicki Y., and Lu P.H. Insights into the structure and substrate interactions of the P-glycoprotein multidrug transporter from spectroscopic studies. Biochim. Biophys. Acta-Biomembranes 1461 (1999) 327-345
12. Sharom F.J., Liu R.H., Qu Q., and Romsicki Y. Exploring the structure and function of the P-glycoprotein multidrug transporter using fluorescence spectroscopic tools. Semin. Cell Dev. Biol. 12 (2001) 257-265
13. Liu R.H., and Sharom F.J. Proximity of the nucleotide binding domains of the P-glycoprotein multidrug transporter to the membrane surface: a resonance energy transfer study. Biochemistry 37 (1998) 6503-6512
14. Liu R.H., and Sharom F.J. Fluorescence studies on the nucleotide binding domains of the P-glycoprotein multidrug transporter. Biochemistry 36 (1997) 2836-2843
15. Liu R.H., Siemiarczuk A., and Sharom F.J. Intrinsic fluorescence of the P-glycoprotein multidrug transporter: sensitivity of tryptophan residues to binding of drugs and nucleotides. Biochemistry 39 (2000) 14927-14938
16. Pawagi A.B., Wang J., Silverman M., Reithmeier R.A.F., and Deber C.M. Transmembrane aromatic amino-acid distribution in P-glycoprotein-a functional-role in broad substrate-specificity. J. Mol. Biol. 235 (1994) 554-564
17. Sonveaux N., Shapiro A.B., Goormaghtigh E., Ling V., and Ruysschaert J.M. Secondary and tertiary structure changes of reconstituted P-glycoprotein-a Fourier transform attenuated total reflection infrared spectroscopy analysis. J. Biol. Chem. 271 (1996) 24617-24624
18. Sonveaux N., Vigano C., Shapiro A.B., Ling V., and Ruysschaert J.M. Ligand-mediated tertiary structure changes of reconstituted P-glycoprotein-a tryptophan fluorescence quenching analysis. J. Biol. Chem. 274 (1999) 17649-17654
19. Manciu L., Chang X.B., Riordan J.R., Buyse F., and Ruysschaert J.M. Nucleotide-induced conformational changes in the human multidrug resistance protein MRP1 are related to the capacity of chemotherapeutic drugs to accumulate or not in resistant cells. FEBS Lett. 493 (2001) 31-35
20. Beaudet L., Urbatsch I.L., and Gros P. High-level expression of mouse Mdr3 P-glycoprotein in yeast Picia pastoris and characterization of ATPase activity. Abc Transporters: Biochemical, Cellular, and Molecular Aspects vol. 292 (1998) 397-413
21. Lerner-Marmarosh N., Gimi K., Urbatsch I.L., Gros P., and Senior A.E. Large scale purification of detergent-soluble P-glycoprotein from Pichia pastoris cells and characterization of nucleotide binding properties of wild-type, Walker A, and Walker B mutant proteins. J. Biol. Chem. 274 (1999) 34711-34718
22. Chifflet S., Torriglia A., Chiesa R., and Tolosa S. A method for the determination of inorganic-phosphate in the presence of labile organic phosphate and high-concentrations of protein-application to lens ATPases. Anal. Biochem. 168 (1988) 1-4
23. Coutinho A., and Prieto M. Ribonuclease-T(1) and alcohol-dehydrogenase fluorescence quenching by acrylamide-a laboratory experiment for undergraduate students. J. Chem. Educ. 70 (1993) 425-428
24. Bradford M.M. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
25. Lacowicz J.R. Principles of Fluorescence Spectroscopy (1999), Kluwer Academic Publishers, New York
26. Castanho M.A.R.B., and Prieto M.J.E. Fluorescence quenching data interpretation in biological systems-the use of microscopic models for data analysis and interpretation of complex systems. Biochim. Biophys. Acta-Biomembranes 1373 (1998) 1-16
27. Connors A.K. Binding Constants: the Measurement of Molecular Complex Stability (1987), John Wiley and Sons, New York
28. Liu R.H., and Sharom F.J. Site-directed fluorescence labeling of P-glycoprotein on cysteine residues in the nucleotide binding domains. Biochemistry 35 (1996) 11865-11873
29. Martin C., Berridge G., Mistry P., Higgins C., Charlton P., and Callaghan R. Drug binding sites on P-glycoprotein are altered by ATP binding prior to nucleotide hydrolysis. Biochemistry 39 (2000) 11901-11906
30. Gans P., Sabatini A., and Vacca A. Investigation of equilibria in solution. Determination of equilibrium constants with the HYPERQUAD suite of programs. Talanta 43 (1996) 1739-1753
31. Urbatsch I.L., Gimi K., Wilke-Mounts S., Lerner-Marmarosh N., Rousseau M.E., Gros P., and Senior A.E. Cysteines 431 and 1074 are responsible for inhibitory disulfide cross-linking between the two nucleotide-binding sites in human P-glycoprotein. J. Biol. Chem. 276 (2001) 26980-26987
32. Baubichon-Cortay H., Baggetto L.G., Dayan G., and Dipietro A. Overexpression and purification of the carboxyl-terminal nucleotide-binding domain from mouse P-glycoprotein-strategic location of a tryptophan residue. J. Biol. Chem. 269 (1994) 22983-22989
33. Shapiro A.B., and Ling V. Positively cooperative sites for drug transport by P-glycoprotein with distinct drug specificities. Eur. J. Biochem. 250 (1997) 130-137
34. Martin C., Berridge G., Higgins C.F., Mistry P., Charlton P., and Callaghan R. Communication between multiple drug binding sites on P-glycoprotein. Mol. Pharmacol. 58 (2000) 624-632
35. Mannering D.E., Sharma S., and Davidson A.L. Demonstration of conformational changes associated with activation of the maltose transport complex. J. Biol. Chem. 276 (2001) 12362-12368
36. Gao M., Cui H.R., Loe D.W., Grant C.E., Almquist K.C., Cole S.P.C., and Deeley R.G. Comparison of the functional characteristics of the nucleotide binding domains of multidrug resistance protein 1. J. Biol. Chem. 275 (2000) 13098-13108
37. Qu Q., Russell P.L., and Sharom F.J. Stoichiometry and affinity of nucleotide binding to P-glycoprotein during the catalytic cycle. Biochemistry 42 (2003) 1170-1177
38. Lamers M.H., Winterwerp H.H.K., and Sixma T.K. The alternating ATPase domains of MutS control DNA mismatch repair. EMBO J. 22 (2003) 746-756
39. Higgins C.F., and Linton K.J. The ATP switch model for ABC transporters. Nat. Struct. Mol. Biol. 11 (2004) 918-926
40. Gatlik-Landwojtowicz E., Aanismaa P., and Seelig A. Quantification and characterization of P-glycoprotein-substrate interactions. Biochemistry 45 (2006) 3020-3032
41. Ambudkar S.V., Kim I.W., and Sauna Z.E. The power of the pump: mechanisms of action of P-glycoprotein (ABCB1). Eur. J. Pharm. Sci. 27 (2006) 392-400