Preparation method for Escherichia coli S30 extracts completely dependent upon tRNA addition to catalyze cell-free protein synthesis

Biotechnology and Bioprocess Engineering

Volumn 11, Issue 5, 2006, Pages 420-424

  Ahn, Jin Ho ^a   Hwang, Mi Yeon ^a   Oh, In Seok ^a   Park, Kyung Moon ^b   Hahn, Geun Hee ^c   Choi, Cha Yong ^a   Kim, Dong Myung ^c  

^a Seoul National University   (South Korea)

^b Hong Ik University   (South Korea)

^c Chungnam National University   (South Korea)

Author keywords

Acetyltransferase; Cell free protein synthesis; Chloramphenicol; Erythropoietin; In vitro suppression; tRNA; Unnatural amino acid

Indexed keywords

AMINO ACID; ETHANOLAMINE; SEPHAROSE; TRANSFER RNA;

ARTICLE; CATALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; PROTEIN SYNTHESIS;

ESCHERICHIA COLI;

EID: 33750688836     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02932309     Document Type: Article

References (29)

1. Noren, C. J., S. J. Anthony-Cahill, M. C. Griffith, and P. G. Schultz (1989) A general method for site-specific incorporation of unnatural amino acids into proteins. Science 244: 182-188.
2. Cook, S. N., W. E. Jack, X. Xiong, L. E. Danley, J. A. Ellman, P. G. Schultz, and C. J. Noren (1995) Photochemically initiated protein splicing. Angew. Chem. Int. Edit. 34: 1629-1630.
3. Miller, J. C., S. K. Silverman, P. M. England, D. A. Dougherty, and H. A. Lester (1998) Flash decaging of tyrosine sidechains in an ion channel. Neuron 20: 619-624.
4. England, P. M., H. A. Lester, N. Davidson, and D. A. Dougherty (1997) Site-specific, photochemical proteolysis applied to ion channels in vivo. Proc. Natl. Acad Sci. USA 94: 11025-11030.
5. Pollitt, S. K. and P. G. Schultz (1998) A photochemical switch for controlling protein-protein interactions. Angew. Chem. Int. Edit. 37: 2104-2107.
6. Cohen, B. E., T. B. McAnaney, E. S. Park, Y. N. Jan, S. G. Boxer, and L. Y. Jan (2002) Probing protein electrostatics with a synthetic fluorescent amino acid. Science 296: 1700-1703.
7. Steward, L. E., C. S. Collins, M. A. Gilmore, J. E. Carlson, J. B. A. Ross, and A. R. Chamberlin (1997) In vitro site-specific incorporation of fluorescent probes into beta-galactosidase. J. Am. Chem. Soc. 119: 6-11.
8. Turcatti, G., K. Nemeth, M. D. Edgerton, U. Meseth, F. Talabot, M. Peitsch, J. Knowles, H. Vogel, and A. Chollet (1996) Probing the structure and function of the tachykinin neurokinin-2 receptor through biosynthetic incorporation of fluorescent amino acids at specific sites. J. Biol. Chem. 271: 19991-19998.
9. Taki, M., T. Hohsaka, H. Murakami, K. Taira, and M. Sisido (2002) Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs. J. Am. Chem. Soc. 124: 14586-14590.
10. Cornish, V. W, D. R. Benson, C. A. Altenbach, K. Hideg, W. L. Hubbell, and P. G. Schultz (1994) Site-specific incorporation of biophysical probes into proteins. Proc. Natl. Acad. Sci. USA 91: 2910-2914.
11. Cornish, V. W, K. M. Hahn, and P. G. Schultz (1996) Site-specific protein modification using a ketone handle. J. Am. Chem. Soc. 118: 8150-8151.
12. Wang, L., Z. W. Zhang, A. Brock, and P. G. Schultz (2003) Addition of the keto functional group to the genetic code of Escherichia coli. Proc. Natl. Acad. Sci. USA 100: 56-61.
13. Wang, L., A. Brock, B. Herberich, and P. G. Schultz (2001) Expanding the genetic code of Escherichia coli. Science 292: 498-500.
14. Chin, J. W, I A. Cropp, J. C. Anderson, M. Mukherji, Z. W. Zhang, and P. G. Schultz (2003) An expanded eukaryotic genetic code. Science 301: 964-967.
15. Taira, H., T. Hohsaka, and M. Sisido (2006) In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system. Nucleic Acids Res. 34: 1653-1662.
16. Hohsaka, T, Y. Ashizuka, H. Murakami, and M. Sisido (2001) Five-base codons for incorporation of nonnatural amino acids into proteins. Nucleic Acids Res. 29: 3646-3651.
17. Kanda, T, K. Takai, T. Hohsaka, M. Sisido, and H. Takaku (2000) Sense codon-dependent introduction of unnatural amino acids into multiple sites of a protein. Biochem. Biophys. Res. Commun. 270: 1136-1139.
18. Hohsaka, T., Y. Ashizuka, H. Taira, H. Murakami, and M. Sisido (2001) Incorporation of nonnatural amino acids into proteins by using various four-base codons in an Escherichia coli in vitro translation system. Biochemistry 40: 11060-11064.
19. Anderson, J. C., T. J. Magliery, and P. G. Schultz (2002) Exploring the limits of codon and anticodon size. Chem. Biol. 9: 237-244.
20. Anderson, J. C., N. Wu, S. W. Santoro, V. Lakshman, D. S. King, and P. G. Schultz (2004) An expanded, genetic code with a functional quadruplet codon. Proc. Natl. Acad. Sci. USA 101: 756-7571.
21. Frankel, A. and R. W. Roberts (2003) In vitro selection for sense codon suppression. RNA 9: 780-786.
22. Jackson, R. J., S. Napthine, and I. Brierley (2001) Development of a tRNA-dependent in vitro translation system. RNA 7: 765-773.
23. Son, J. M., J. H. Ahn, M. Y. Hwang, C. G. Park, C. Y. Choi, and D. M. Kim (2006) Enhancing the efficiency of cell-free protein synthesis through the polymerase-chain-reaction-based addition of a translation enhancer sequence and the in situ removal of the extra amino acid residues. Anal. Biochem. 351: 187-192.
24. Kim, D. M., T. Kigawa, C. Y. Choi, and S. Yokoyama (1996) A highly efficient cell-free protein synthesis system from Escherichia coli. Eur J. Biochem. 239: 881-886.
25. Nakano, H., R. Okumura, C. Goto, and T Yamane (2002) In vitro combinatorial mutagenesis of the 65th and 222nd positions of the green fluorescent protein of Aequarea victoria. Biotechnol. Bioprocess Eng. 7: 311-315.
26. Lee C.-S., S.-H. Lee, Y.-G. Kim, C.-H. Choi, Y.-K. Kim, and B.-G. Kim (2006) Biochemical reactions on a micro-fluidic chip based on a precise fluidic handling method at the nanoliter scale. Biotechnol. Bioprocess Eng. 11: 146-153.
27. Park, Y. S., S. H. Hwang, and C.-Y. Choi (2005) Construction of CpG motif-enriched DNA vaccine plasmids for enhanced early immune response. Biotechnol. Bioprocess Eng. 10: 29-33.
28. Kim, D.-M., C.,-Y. Choi, J., H.Ahn,.T.-W., Kim, N.-Y. Kim, I-S. Oh, and C.-G. Park (2006) Development of a rapid and productive cell-free protein synthesis system. Biotechnol. Bioprocess Eng. 11: 235-239.
29. Schagger, H. and G. von Jagow (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166: 368-379.