Development of a tRNA-dependent in vitro translation system

RNA

Volumn 7, Issue 5, 2001, Pages 765-773

  Jackson, R J ^a   Napthine, S ^a   Brierley, I ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Leaky termination; Programmed ribosomal frame shifting; Rabbit reticulocyte lysate; Wheat germ system

Indexed keywords

TRANSFER RNA;

AFFINITY CHROMATOGRAPHY; ARTICLE; PRIORITY JOURNAL; RETICULOCYTE LYSATE; RIBOSOMAL FRAMESHIFTING; RNA TRANSLATION; STOP CODON; WHEAT GERM;

ANIMALS; PROTEIN BIOSYNTHESIS; RABBITS; RETICULOCYTES; RNA, MESSENGER; RNA, TRANSFER; TRITICUM;

ORYCTOLAGUS CUNICULUS; TRITICUM AESTIVUM;

EID: 0035043155     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838201002539     Document Type: Article

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5. An introduction to polyacrylamide gel electrophoresis
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7. The use of affinity chromatography on 2′5′ ADP-sepharose reveals a requirement for NADPH, thioredoxin and thioredoxin reductase for the maintenance of high protein synthesis activity in rabbit reticulocyte lysates
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9. The roles of sugar phosphates and thiol-reducing systems in the control of reticulocyte protein synthesis
10. Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic mRNA
11. Messenger RNA translation in the presence of homologous and heterologous tRNA
12. The Q-base of asparaginyl-tRNA is dispensable for efficient -1 ribosomal frame-shifting in eukaryotes
13. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
14. Preparation of a tRNA-dependent wheat germ protein-synthesizing system
15. Direct analysis of aminoacylation levels of tRNAs in vivo. Application to studying recognition of Escherichia coli initiator tRNA mutants by glutaminyl-tRNA synthetase
16. Cloning and expression of influenza virus genes