Influence of the SCGE protocol on the amount of basal DNA damage detected in the Mediterranean mussel, Mytilus galloprovincialis

Environmental and Molecular Mutagenesis

Volumn 47, Issue 8, 2006, Pages 579-586

  Machella, Nicola ^a,b   Battino, Maurizio ^a   Pisanelli, Barbara ^a   Regoli, Francesco ^a  

^a UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

^b Columbia University ^*  (United States)

Author keywords

Comet assay; Dicentrarchus labrax; Genotoxicity; Helix aspersa; Mytilus galloprovincialis

Indexed keywords

AURINTRICARBOXYLIC ACID; CALCIUM ION; EDETIC ACID; MAGNESIUM ION; NUCLEASE; PROTEINASE K; TROLOX C;

ANIMAL CELL; ARTICLE; BLOOD CELL; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA EXTRACTION; DNA STRAND BREAKAGE; EUROPEAN SEA BASS; GEL ELECTROPHORESIS; GENOTOXICITY; HELIX ASPERSA; MYTILUS GALLOPROVINCIALIS; NONHUMAN;

ANIMALS; AURINTRICARBOXYLIC ACID; BUFFERS; CHROMANS; COMET ASSAY; DNA BREAKS; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; EDETIC ACID; ENDOPEPTIDASE K; ENVIRONMENTAL MONITORING; HELIX (SNAILS); HEMOCYTES; MUTAGENICITY TESTS; MYTILUS; SENSITIVITY AND SPECIFICITY;

DICENTRARCHUS LABRAX; GASTROPODA; HELIX ASPERSA; INVERTEBRATA; MYTILUS GALLOPROVINCIALIS; TELEOSTEI; VERTEBRATA;

EID: 33750683592     PISSN: 08936692     EISSN: 10982280     Source Type: Journal    
DOI: 10.1002/em.20231     Document Type: Article

References (40)

1. Bailey SM, Comforth MN, Ullrich RL, Goodwin EH. 2004. Dysfunctional mammalian telomeres join with DNA double-strand breaks. DNA Repair 3:349-357.
2. Banerjee S, Smallwood A, Hultén M. 1995. ATP-dependent reorganization of human sperm nuclear chromatin. J Cell Sci 108:755-765.
3. Battino M, Ferreiro MS, Armeni T, Politi A, Bompadre S, Massoli A, Bullon P. 2005. In vitro antioxidant activities of antioxidant-enriched toothpastes. Free Radic Res 39:343-350.
4. Bhattacharya R, Lakshmana Rao PV. 2001. Pharmacological interventions of cyanid-induced cytotoxicity and DNA damage in isolated rat thymocytes and their protective efficacy in vivo. Toxicol Lett 119:59-70.
5. Burton RF. 1983. Ionic regulation and water balance, In: Saleuddin ASM, Wilbur KM, editors. The Mollusca, Vol. 5: Physiology, Part 2. New York: Academic Press, pp 291-352.
6. Collins AR. 2002. The comet assay, principles, applications and limitations. In: Didenko W, editor. Methods in Molecular Biology, Vol. 203: In Situ Detection of DNA Damage: Methods and Protocols. Totowa, NJ: Humana Press, pp 163-177.
7. Cotelle S, Férard JF. 1999. Comet assay in genetic ecotoxicology: A review. Environ Mol Mutagen 34:246-255.
8. Dixon DR, Pruski AM, Dixon LRJ, Jha AN. 2002. Marine invertebrates eco-genotoxicology: A methodological overview. Mutagen 17:495-507.
9. Dixon DR, Pruski AM, Dixon LRJ. 2004. The effects of hydrostatic pressure change on DNA integrity in the hydrothermal-vent mussel Bathymodiolus azoricus: Implications for future deep-sea mutagenicity studies. Mutat Res 552:235-246.
10. Everaarts JM. 1995. DNA integrity as a biomarker of marine pollution: Strand breaks in seastar (Asterias rubens) and dab (Limanda limanda). Mar Pollut Bull 31:431-438.
11. Fairbain DW, Reyes WA, O'Neill KL. 1994. Alkali-labile sites are prevalent in kidney tissue DNA. Cancer Lett 81:67-76.
12. Fairbairn DW, Olive PL, O'Neill KL. 1995. The comet assay: A comprehensive review. Mutat Res 339:37-59.
13. Fernandez JL, Vazquez-Gundin F, Rivero MT, Genesca A, Gosalvez J, Goyanes V. 2001. DBD-fish on neutral comets: Simultaneous analysis of DNA single- and double-strand breaks in individual cells. Exp Cell Res 270:102-109.
14. Frenzilli G, Scarcelli V, Del Barga I, Nigro M, Forlin L, Bolognesi C, Sturve J. 2004. DNA damage in eelpout (Zoarces viviparus) from Goteborg harbour. Mutat Res 552:187-195.
15. Gielazyn ML, Ringwood AH, Piegorsch WW, Stancyk SE. 2003. Detection of oxidative DNA damage in isolated marine bivalve haemocytes using the comet assay and formamidopyrimidine glycosylase (Fpg). Mutat Res 452:15-22.
16. Haines G, Marples B, Daniel P, Morris I. 1998. DNA damage in human and mouse spermatozoa after in vitro-irradiation assessed by the come assay. Adv Exp Med Biol 444:79-91.
17. Kirschner LB. 1991. Water and ions. In: Ladd Prosser C, editor. Environmental and Metabolic Animal Physiology. New York: Wiley-Liss. pp 13-107.
18. Lee RF, Steinert SA. 2003. Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutat Res 544:43-64.
19. Machella N, Regoli F, Cambria A, Santella RM. 2004. Application of an immunoperoxidase staining method for detection of 7,8-dihydro-8- oxodeoxyguanosine as a biomarker of chemical-induced oxidative stress in marine organisms. Aquat Toxicol 67:23-32.
20. Meneghini R. 1997. Iron homeostasis, oxidative stress, and DNA damage. Free Rad Biol Med 23:783-792.
21. Mitchelmore CL, Chipman JK. 1998. DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutat Res 399:135-147.
22. Mitchelmore CL, Birmenlin C, Chipman JK, Livigstone DR. 1998a. Evidence for cytochrome P-450 catalysis and free radical involvement in the production of DNA strand breaks by benzo[a]pyrene and nitroaromatics in mussel (Mytilus edulis L.) digestive gland cells. Aquat Toxicol 41:193-212.
23. Mitchelmore CL, Birmenlin C, Livigstone DR, Chipman JK. 1998b. Detection of DNA strand breaks in isolated mussel (Mytilus edulis L.) digestive gland cells using the "Comet" assay. Ecotoxicol Environ Saf 41:51-58.
24. Nacci DE, Nelson S, Nelson W, Jackim E. 1992. Application of the DNA alkaline unwinding assay to detect DNA strand breaks in marine bivalves. Mar Environ Res 33:83-100.
25. Östling O, Johanson KJ. 1984. Microelectrophoretic study of radiation-induced DNA damage in individual mammalian cells. Biochem Biophys Res Commun 123:291-298.
26. Peréz-Cadahía B, Laffon B, Pásaro E, Méndez J. 2004. Evaluation of PAH bioaccumulation and DNA damage in mussels (Mytilus galloprovincialis) exposed to spilled Prestige crude oil. Comp Biochem Physiol C 138:453-460.
27. Regoli F, Frenzilli G, Bocchetti R, Annarumma F, Scarcelli V, Fattorini D, Nigro M. 2004. Time-course variations of oxyradical metabolism, DNA integrity and lysosomal stability in mussels, Mytilus galloprovincialis, during a field translocation experiment. Aquat Toxicol 68:167-178.
28. 2+-dependent endonuclease from human spleen: Purification, properties, and role in apoptosis. Biochemistry 32:9129-9136.
29. Rivero MT, Vazquez-Gundin F, Goyanes V, Campos A, Blasco M, Gosalvez J, Fernandez JL. 2001. High frequency of constitutive alkali-labile sites in mouse major satellite DNA, detected by DNA breakage detection-fluorescence in situ hybridization. Mutat Res 483:43-50.
30. Sakkas D, Moffatt O, Manicardi GC, Mariethoz E, Tarozzi N, Bizzaro D. 2002. Nature of DNA damage in ejaculated human spermatozoa and the possible involvement of apoptosis. Biol Reprod 66:1061-1067.
31. Singh NP. 2000. A simple method for accurate estimation of apoptotic cells. Exp Cell Res 256:328-337.
32. Singh NP, Danner BD, Tice RR, Mc Coy MT, Collins GD, Schneider EL. 1989. Abundant alkali-sensitive sites in DNA of human and mouse sperm. Exp Cell Res 184:461-470.
33. Singh NP, Muller CH, Berger RE. 2003. Effects of age on DNA double-strand breaks and apoptosis in human sperm. Fertil Steril 80:1420-1430.
34. Siu WHL, Cao J, Jack RW, Wu RSS, Richardson BJ, Xu L, Lam PKS. 2004. Application of the comet and micronucleus assay to the detection of B[a]P genotoxicity in haemocytes of the green-lipped mussel (Perna viridis). Aquat Toxicol 66:381-392.
35. Steinert SA. 1996. Contribution of apoptosis to observed DNA damage in mussel cells. Mar Environ Res 42:253-259.
36. Steinert SA. 1999. DNA damage as a bivalve biomarker. Biomarkers 4: 492-496.
37. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. 2000. Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206-221.
38. Wang YC, Cheung RH, Tung LC. 2004. Comparison of the cytotoxicity induced by different exposure to sodium arsenate in two fish cells lines. Aquat Toxicol 69:67-79.
39. Wilson JT, Pascoe PL, Parry JM, Dixon DR. 1998. Evaluation of the comet assay as a method for the detection of DNA damage in the cells of a marine invertebrate, Mytilus edulis L. (Mollusca: Pelecypoda). Mutat Res 399:87-95.
40. Winston GW, Moore MN, Kirkin MA, Soverchia C. 1996. Production of reactive species by haemocytes from the marine mussel, Mytilus edulis: Lysosomal localization and effects of xenobiotics. Comp Biochem Physiol C 2:221-229.