Identification of single Mn2+ binding sites required for activation of the mutant proteins of E. coli RNase HI at Glu48 and/or Asp134 by X-ray crystallography

Journal of Molecular Biology

Volumn 345, Issue 5, 2005, Pages 1171-1183

  Tsunaka, Yasuo ^a   Takano, Kazufumi ^a,b   Matsumura, Hiroyoshi ^a   Yamagata, Yuriko ^c   Kanaya, Shigenori ^a  

^a OSAKA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c KUMAMOTO UNIVERSITY   (Japan)

Author keywords

active site mutants; metal fluctuation model; RNase H; X ray crystallography

Indexed keywords

BACTERIAL ENZYME; MANGANESE; MUTANT PROTEIN; RIBONUCLEASE; RIBONUCLEASE HI; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; X RAY CRYSTALLOGRAPHY;

ESCHERICHIA COLI;

EID: 11844284013     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2004.11.007     Document Type: Article

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