Comparison of different methods of isolation of DNA of commonly encountered Candida species and its quantitation by using a real-time PCR-based assay

Journal of Clinical Microbiology

Volumn 42, Issue 7, 2004, Pages 3159-3163

  Maaroufi, Younes ^a   Ahariz, Naima ^a   Husson, Mireille ^a   Crokaert, Françoise ^a  

^a JULES BORDET INSTITUTE   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINE SERUM ALBUMIN; CHLOROFORM; GUANIDINE DERIVATIVE; PHENOL; PHENOL DERIVATIVE; PROTEINASE K; REAGENT; TAQ POLYMERASE; TRIS REAGENT; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; CANCER PATIENT; CANDIDA; CELL WALL; CLINICAL ARTICLE; COST; DIAGNOSTIC KIT; DNA DETERMINATION; DNA EXTRACTION; DNA ISOLATION; DNA TEMPLATE; FUNGUS CULTURE; GENE AMPLIFICATION; HEMATOLOGY; HUMAN; MEMBRANE DAMAGE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; TECHNIQUE;

CANDIDA; DNA, FUNGAL; FUNGEMIA; HUMANS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC;

BOVINAE; CANDIDA; FUNGI;

EID: 3142682262     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.7.3159-3163.2004     Document Type: Article

References (16)

1. Ahmad, S., Z. Khan, S. Mustafa, and U. Khan. 2002. Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification. J. Clin. Microbiol. 40:2483-2489.
2. Ascioglu, S., J. H. Rex, B. de Pauw, J. E. Bennett, J. Bille, F. Crokaert, D. W. Denning, J. P. Donnelly, J. E. Edwards, Z. Erjavec, D. Fiere, O. Lortholary, J. Maertens, J. F. Meis, T. F. Patterson, J. Ritter, D. Selleslag, P. M. Shah, D. A. Stevens, and T. J. Walsh. 2002. Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus. Clin. Infect. Dis. 34:7-14.
3. Bougnoux, M.-E., C. Dupont, J. Mateo, P. Saulnier, V. Faivre, D. Payen, and M. H. Nicolas-Chanoine. 1999. Serum is more suitable than whole blood for diagnosis of systemic candidiasis by nested PCR. J. Clin. Microbiol. 37:925-930.
4. Burnie, J. P., N. Golbang, and R. C. Matthews. 1997. Semiquantitative polymerase chain reaction enzyme immunoassay for diagnosis of disseminated candidiasis. Eur. J. Clin. Microbiol. Infect. Dis. 16:346-350.
5. Chryssanthou, E., B. Anderson, B. Petrini, S. Löfdahl, and J. Tollemar. 1994. Detection of Candida albicans DNA in serum by polymerase chain reaction. Scand. J. Infect. Dis. 26:479-485.
6. Dixon, S. C., J. Horti, Y. Guo, E. Reed, and W. D. Figg. 1998. Methods for extracting and amplifying genomic DNA isolated from frozen serum. Nat. Biotechnol. 16:91-94.
7. Flahaux, M., D. Sanglard, M. Monod, J. Bille, and M. Rossier. 1998. Rapid detection of Candida albicans in clinical samples by DNA amplification of common regions from C. albicans-secreted aspartic proteinase genes. J. Clin. Microbiol. 36:395-401.
8. Horvath, L. L., D. R. Hospenthal, C. K. Murray, and D. P. Dooley. 2003. Detection of simulated candidemia by the BACTEC 9240 system with Plus aerobic/F and anaerobic/F blood culture bottles. J. Clin. Microbiol. 41:4714-4717.
9. Khan, Z. U., and A. S. Mustafa. 2001. Detection of Candida species by polymerase chain reaction (PCR) in blood samples of experimentally infected mice and patients with suspected candidemia. Microbiol. Res. 156:95-102.
10. Löeffler, J., H. Hebart, U. Schumacher, H. Reitze, and H. Einsele. 1997. Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood. J. Clin. Microbiol. 35:3311-3312.
11. Löeffler, J., N. Henke, H. Hebart, D. Schmidt, L. Hagmeyer, U. Schumacher, and H. Einsele. 2000. Quantification of fungal DNA by using fluorescence resonance energy transfer and the LightCycler system. J. Clin. Microbiol. 38:586-590.
12. Maaroufi, Y., C. Heymans, J.-M. De Bruyne, V. Duchateau, H. Rodriguez-Villalobos, M. Aoun, and F. Crokaert. 2003. Rapid detection of Candida albicans in clinical blood samples by using a TaqMan-based PCR assay. J. Clin. Microbiol. 41:3293-3298.
13. Sandhu, G. S., B. C. Kline, L. Stockman, and G. D. Roberts. 1995. Molecular probes for diagnosis of fungal infections. J. Clin. Microbiol. 33:2913-2919.
14. Shin, J. H., F. S. Nolte, B. P. Holloway, and C. J. Morrison. 1999. Rapid identification of up to three Candida species in a single reaction tube by a 5′ exonuclease assay using fluorescent DNA probes. J. Clin. Microbiol. 37:165-170.
15. Turenne, C. Y., S. E. Sanche, D. J. Hoban, J. A. Karlowsky, and A. M. Kabani. 1999. Rapid identification of fungi by using the ITS2 genetic region and an automated fluorescent capillary electrophoresis system. J. Clin. Microbiol. 37:1846-1851.
16. Wahyuningsih, R., H.-J. Freisleben, H.-G. Sonntag, and P. Schnitzler. 2000. Simple and rapid detection of Candida albicans DNA in serum by PCR for diagnosis of invasive candidiasis. J. Clin. Microbiol. 38:3016-3021.