Glutamine regulates expression of key transcription factor, signal transduction, metabolic gene, and protein expression in a clonal pancreatic β-cell line

Journal of Endocrinology

Volumn 190, Issue 3, 2006, Pages 719-727

  Corless, Mary ^b   Kiely, Aoife ^b   McClenaghan, Neville H ^a   Flatt, Peter R ^a   Newsholme, Philip ^b  

^a UNIVERSITY OF ULSTER   (United Kingdom)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; CALCINEURIN; DNA; GLUTAMATE RECEPTOR AGONIST; GLUTAMIC ACID; GLUTAMINE; GLUTATHIONE; INSULIN; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; NITRITE; PHOSPHATASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR PDX 1; UREA;

ARTICLE; CELL CLONING; CONTROLLED STUDY; DNA MICROARRAY; DOWN REGULATION; ENZYME ACTIVATION; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; INSULIN RELEASE; METABOLIC REGULATION; NUCLEOTIDE SEQUENCE; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; UPREGULATION;

BLOTTING, WESTERN; CALCINEURIN; CLONE CELLS; DEPRESSION, CHEMICAL; ELECTROPHORETIC MOBILITY SHIFT ASSAY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GLUTAMIC ACID; GLUTAMINE; HOMEODOMAIN PROTEINS; HUMANS; INSULIN; INSULIN-SECRETING CELLS; N-METHYLASPARTATE; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; TIME FACTORS; TRANS-ACTIVATORS; TRANSCRIPTION FACTORS;

EID: 33749861808     PISSN: 00220795     EISSN: None     Source Type: Journal    
DOI: 10.1677/joe.1.06892     Document Type: Article

References (40)

1. Ammon HPT, Grimm A, Lutz S, Wagner-Teschner D, Händel M & Hagenloh I 1980 Islet glutathione, and insulin release. Diabetes 830 834.
2. Averous J, Bruhat A, Mordier S & Fafournoux P 2003 Recent advances in the understanding of amino acid regulation of gene expression. Journal of Nutrition 133 2040S-2045S.
3. Avila JG, Tsujimura T, Oberholzer J, Churchill T, Salehi P, Shapiro AMJ & Lakey JRT 2003 Improvement of pancreatic islet isolation outcomes using glutamine perfusion during isolation procedure. Cell Transplantation 12 877-881.
4. Avila J, Barbaro B, Gangemi A, Romagnoli T, Kuechle J, Hansen M, Shapiro J, Testa G, Sankary H, Benedetti E et al. 2005 Intraductal glutamine administration reduces oxidative injury during human pancreatic islet isolation. American Journal of Transplantation 5 2830-2837.
5. Baker MA, Cerniglia GJ & Zaman A 1990 Microtiter plate assay for the Measurement of glutathione and glutathione disul in large numbers of biological samples. Annals of Biochemistry 190 360-365.
6. Bertrand G, Ishiyama N, Nenquin M, Ravier MA & Henquin J-C 2002 The elevation of glutamate content and the amplification of insulin secretion in glucose-stimulated pancreatic islets are not causally related. Journal of Biological Chemistry 277 32883-32891.
7. Brennan L, Shine A, Hewage C, Malthouse JPG, Brindle K, McClenaghan N, Flatt PR & Newsholme P 2002 A NMR based demonstration of substantial oxidative L-alanine metabolism and L-alanine enhanced glucose metabolism in a clonal pancreatic bete-cell line - metabolism of L-alanine is important to the regulation of insulin secretion. Diabetes 51 1714-1721.
8. 13C NMR analysis reveals a link between L-glutamine metabolism, D-glucose metabolism and gamma-glutamyl cycle activity in a clonal pancreatic beta-cell line. Diabetologia 46 1512-1521.
9. Brice NL, Varadi A, Ashcroft SJ & Molnar E 2002 Metabotropic glutamate and GABA(B) receptors contribute to the modulation of glucose-stimulated insulin secretion in pancreatic beta cells. Diabetologia 45 242-252.
10. Curi R, Lagranha CJ, Doi SQ, Sellitti F, Procopio J, Pithon-Curi TC, Corless M & Newsholme P 2005 Molecular mechanisms of glutamine action. Journal of Cellular Physiology 204 392-401.
11. Dingledine R, Borges K, Bowie D & Traynelis SF 1999 The glutamate receptor ion channels. Pharmacological Reviews 7 61.
12. Dixon G, Nolan J, McClenaghan NH, Flatt PR & Newsholme P 2003 A comparative study of amino acid consumption by rat islet cells and the clonal beta-cell line BRIN-BD11 - the functional significance of L-alanine. Journal of Endocrinology 179 447-454.
13. Gao Z-Y, Li G, Najafi H, Wolf BA & Matschinski FM 1999 Glucose regulation of glutaminolysis and its role in insulin secretion. Diabetes 48 1535-1542.
14. Gauthier BR, Brun T, Sarret EJ, Ishihara H, Schaad O, Descombes P & Wollheim CB 2004 Oligonucleotide microarray analysis reveals PDX1 as an essential regulator of mitochondrial metabolism in rat islets. Journal of Biological Chemistry 279 31121-31130.
15. Haber EP, Ximenes HM, Procopio J, Carvalho CR, Curi R & Carpinelli A 2003 Pleiotropic effects of fatty acids on pancreatic beta-cells. Journal of Cellular Physiology 194 1-12.
16. Haussinger D 1996 The role of cellular hydration in the regulation of cell function. Biochemical Journal 313 697-710.
17. 2+ dependent phosphatase. Nature 369 235-239.
18. MacDonald MJ & Fahien LA 2000 Glutamate is not a messenger in insulin secretion. Biochemical Journal 275 34025-34027.
19. McClenaghan NH, Barnett CR, O'Harte FPM & Flatt PR 1996 Mechanisms of amino acid-induced insulin secretion from the glucose-responsive BRIN-BD11 pancreatic β-cell line. Journal of Endocrinology 15 349-357.
20. MacMullen C, Fang J, Hsu BYL, Kelly A, De Lonlay-Debeney P, Saudubray J-M, Ganguly A, Smith TJ & Stanley CA 2001 The hyperinsulinism/ hyperammonemia syndrome in children with regulatory mutations in the inhibitory guanosine triphosphate binding domain of glutamate dehydrogenase. Journal of Clinical Endocrinology and Metabolism 86 1782-1787.
21. Maechler P & Wollheim CB 1999 Mitochondrial glutamate acts as a messenger in glucose-induced insulin exocytosis. Nature 402 595-596
22. Malaisse WJ, Sener A, Malaisse-Lagae F, Welsh M, Matthews DE, Bier DM & Hellerstrom C 1982a The stimulus-secretion coupling of amino acid-induced insulin release: metabolic response of pancreatic islets of L-glutamine and L-leucine. Journal of Biological Chemistry 257 8731-8737.
23. Malaisse WJ, Sener A, Malaisse-Lagae F, Welsh M, Matthews DE, Bier DM & Hellerstrom C 1982b The stimulus-secretion coupling of amino acid-induced insulin release. Journal of Biological Chemistry 257 8731-8737.
24. Molnar E, Varadi A, McIlhinney RAJ & Ashcroft SJH 1995 Identification of functional ionotropic glutamate receptor proteins in pancreatic beta cells and in islets of Langerhans. FEBS Letter 371 253-257.
25. Murphy C & Newsholme P 1998 The importance of glutamine metabolism in murine macrophages and human monocytes to L-arginine biosynthesis and rates of nitrite or urea production. Clinical Science 95 397-407.
26. Newsholme P, Procopio J, Ramos Lima MM, Pithon-Curi TC & Curi R 2003 Glutamine and glutamate - their central role in cell metabolism and function. Cell Biochemistry and Function 21 1-9.
27. Prentki M & Renold AE 1983 Neutral amino acid transport in isolated rat pancreatic islets. Journal of Biological Chemistry 258 14239-14244.
28. Raschaert J, Flatt PR, Barnett CR, McClenaghan NH & Malaisse WJ 1996a D-Glucose metabolism in BRIN-BD11 islet cells. Biochemical and Molecular Medicine 57 97-105.
29. Rasschaert J, Flatt PR, McClenaghan NH & Malaisse WJ 1996b Amino acid oxidation in BRIN-BD11 islet cells. Medical Science Research 24 691-692.
30. Renstrom E, Ding WG, Bokvist K & Rorsman P 1996 Neurotransmitter-induced inhibition of exocytosis in insulin-secreting beta cells by activation of calcineurin. Neuron 17 513-522.
31. Schuit F, Halban P & Rhodes C 2005 What is a beta cell and how can we improve it? Diabetologia 48 R93-R101.
32. Sener A, Mercan D & Malaisse WJ 2001 Enzymic activities in two populations of purified rat islet beta-cells. International Journal of Molecular Medicine 8 285-289.
33. Stanley CA, Lieu YK, Hsu BY, Burlina AB, Greenberg CR, Hopwood NJ, Perlman K, Rich BH, Zammarchi E & Poncz M 1998 Hyperinulinism and hyperammonemia in infants with regulatory mutations of the glutamate dehydrogenase gene. New England Journal of Medicine 338 1352-1357.
34. Stanley CA, Fang J, Kutyna K, Hsu BYL, Ming JE, Glaser B & Poncz M 2000 Molecular basis and characterization of the hyperinsulinism/ hyperammonemia syndrome: predominance of mutations in exons 11 and 12 of the glutamate dehydrogenase gene. Diabetes 49 667-673.
35. Tanizawa Y, Nakai K, Sasaki T, Anno T, Ohta Y, Inoue H, Matsuo K, Koga M, Furukawa S & Oka Y 2002 Unregulated elevation of glutamate dehydrogenase activity induces glutamine-stimultaed insulin secretion-identification and characterization of a GLUD1 gene mutation and insulin secretion studies with MIN6 cells overexpressing the mutant glutamate dehydrogenase. Diabetes 51 712-717.
36. Tietze F 1969 Enzymic method for quantitative determination of nanogram amounts of total and oxidize glutathione: applications to mammalian blood and other tissues. Annals of Biochemistry 27 502-522.
37. Webb GC, Akbar MS, Zhao C & Steiner DF 2000 Expression Profiling of pancreatic beta cells: glucose regulation of secretory and metabolic pathway genes. PNAS 97 5773-5778.
38. Webb GC, Akbar MS, Zhao C & Steiner DF 2001 Expression Profiling of pancreatic beta cells. Glucose regulation of secretory and metabolic pathway genes. Diabetes 50 S135-S136.
39. Wollheim CB 2000 Beta-cell mitochondria in the regulation of insulin secretion: a new culprit in type II diabetes. Diabetologia 43 265-277.
40. Yaney GC & Corkey BE 2003 Fatty acid metabolism and insulin secretion in pancreatic beta cells. Diabetologia 46 1297-1312.