Restoration of oxacillin susceptibility in methicillin-resistant staphylococcus aureus by blocking the MecR1-mediated signaling pathway

Journal of Chemotherapy

Volumn 18, Issue 4, 2006, Pages 360-365

  Meng, Jingru ^a   Hu, Benquan ^a   Liu, Jie ^a   Hou, Zheng ^a   Meng, Jia ^a   Jia, Min ^a   Luo, Xiaoxing ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Antisense phosphothioate oligonucleotide; MecR1; MRSA; PBP2a; PS ODN; Staphylococcus aureus

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; BACTERIAL PROTEIN; BETA LACTAM ANTIBIOTIC; MESSENGER RNA; OXACILLIN; PENICILLIN BINDING PROTEIN 2A; PROTEIN INHIBITOR; PROTEIN MECR1; RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; BACTERIUM COLONY; CONTROLLED STUDY; DRUG EFFECT; DRUG SENSITIVITY; GENE TARGETING; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; PROTEIN TARGETING;

EID: 33749007922     PISSN: 1120009X     EISSN: None     Source Type: Journal    
DOI: 10.1179/joc.2006.18.4.360     Document Type: Article

References (24)

1. Tiemersma EW, Bronzwaer SL, Lyytikäinen O, et al. Methicillin-resistant Staphylococcus aureus in Europe, 1999-2002. Emerg Infect Dis 2004; 10(9): 1627-1634.
2. Ko KS, Lee JY, Sun JY, Oh WS, Peck KR, Lee NY, Song JH. Distribution of major genotypes among methicillin-resistant Stophylococcus aureus clones in Asian countries. J Clin Microbiol. 2005;43(1):421-426.
3. Weber JT. Community-associated methicillin-resistant Staphylococcus aureus. liHn Infect Dis 2005; 41(Suppl 4): S269-72.
4. Klevens RM, Edwards JR, Tenover FC, McDonald LC, Horan T, Gaynes R. Changes in the epidemiology of methicillin-resistant Staphylococcus aureus in intensive care units in US hospitals, 1992-2003. Clin Infect Dis 2006; 2(3): 389-391
5. White C. MRSA infections rose by 5% between 2003 and 2004. Br Med J 2004; 329(7458): 131.
6. Archer GL, Bosilevac JM. Signaling antibiotic resistance in staphylococci. Science 2001; 291(5510): 1915-1916.
7. Zhang HZ, Hackbarth CJ, Chansky KM, Chambers HF. A proteolytic transmembrane signaling pathway and resistance to beta-lactams in staphylococci. Science 2001; 291(5510): 1962-1965.
8. Niemeyer DM, Pucci MJ, Thanassi JA, Sharma VK, Archer GL. Role of mecA transcriptional regulation in the phenotypic expression of methicillin resistance in Staphylococcus aureus. J Bacteriol 1996; 178(18): 5464-5471.
9. Rosato AE, Kreiswirth BN, Craig WA, Eisner W, Climo MW, Archer GL. mecA-blaZ corepressors in clinical Staphylococcus aureus isolates. Antimicrob Agents Chemother 2003; 47(4): 1460-1463.
10. Archer GL, Niemeyer DM, Thanassi JA, Pucci MJ. Dissemination among staphylococci of DNA sequences associated with methicillin resistance. Antimicrob Agents Chemother 1994; 38(3): 447-454.
11. Finan JE, Rosato AE, Dickinson TM, Ko D, Archer GL. Conversion of oxacillin-resistant staphylococci from heterotypic to homotypic resistance expression. Antimicrob Agents Chemother 2002; 46(1): 24-30.
12. Sharma RC, Schimke RT. Preparation of electrocompetent E. coli using salt-free growth medium. Biotechniques 1996; 20(1): 42-44.
13. Brakstad OG, Maeland JA. Mechanisms of methicillin resistance in staphylococci. APMIS 1997; 105(4): 264-276.
14. Weller TMA. The distribution of mecA, mecR1 and mecI and sequence analysis of mecI and mec promoter region in staphylococci expressing resistance to methicillin. J Antimicrob Chemother 1999; 43(1): 15-22.
15. Sharma VK, Hackbarth CJ, Dickinson TM, Archer GL. Interaction of native and mutant MecI repressors with sequences that regulate mecA, the gene encoding penicillin binding protein 2a in methicillin-resistant staphylococci. J Bacteriol 1998; 180(8): 2160-2166.
16. Katayama Y, Ito T, Hiramatsu K. Genetic organization of the chromosome region surrounding mecA in clinical staphylococcal strains: role of IS431-mediated mecI deletion in expression of resistance in mecA-carrying, low-level methicillin-resistant Staphylococcus haemolyticus. Antimicrob Agents Chemother 2001; 45(7): 1955-1963.
17. Kuwahara-Arai K, Kondo N, Hori S, Tateda-Suzuki E, Hiramatsu K. Suppression of methicillin resistance in a mecA-containing pre-methicillin- resistant Staphylococcus aureus strain is caused by the mecI-mediated repression of PBP 2′ production. Antimicrob Agents Chemother 1996; 40(12): 2680-2685.
18. Harth G, Zamecnik PC, Tang JY, Tabatadze D, Horwitz MA. Treatment of Mycobacterium tuberculosis with antisense oligonucleotides to glutamine synthetase mRNA inhibits glutamine synthetase activity, formation of the poly-L-glutamate/glutamine cell wall structure, and bacterial replication. Proc Natl Acad Sci USA 2000; 97(1): 418-423.
19. White DG, Maneewannakul K, Von Hofe E et al. Inhibition of the multiple antibiotic resistance (mar) operon in Escherichia coli by antisense DNA analogs. Antimicrob Agents Chemother 1997; 41(12): 2699-2704.
20. Good L, Nielsen PE. Antisense inhibition of gene expression in bacteria by PNA targeted to mRNA. Nat Biotechnol. 1998; 16(4): 355-358.
21. Fillion P, Desjardins A, Sayasith K, Lagace J. Encapsulation of DNA in negatively charged liposomes and inhibition of bacterial gene expression with fluid liposome-encapsulated antisense oligonucleotides. Biochim Biophys Acta 2001; 1515(1): 44-54.
22. Jones MN. Use of liposomes to deliver bactericides to bacterial biofilms. Methods Enzymol 2005; 391:211-228.
23. Suzuki E, Kuwahara-Arai K, Richardson JF, Hiramatsu K. Distribution of mec regulator genes in methicillin-resistant Staphylococcus clinical strains. Antimicrob Agents Chemother 1993; 37(6): 1219-1226.
24. Petinaki E, Arvanti A, Dimitracopoulos G, Spiliopoulou I. Detection of mecA, mecR1 and mecI genes among clinical isolates of methicillin-resistant staphylococci by combined polymerase chain reactions. J Antimicrob Chemother 2001; 47(3): 297-304.