Suppression of methicillin resistance in a mecA-containing pre- methicillin-resistant Staphylococcus aureus strain is caused by the mecI- mediated repression of PBP 2' production

Antimicrobial Agents and Chemotherapy

Volumn 40, Issue 12, 1996, Pages 2680-2685

  Kuwahara Arai, Kyoko ^a   Kondo, Noriko ^a   Hori, Satoshi ^a   Tateda Suzuki, Eiko ^a   Hiramatsu, Keiichi ^a  

^a JUNTENDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC RESISTANCE; ARTICLE; CATABOLITE REPRESSION; CULTURE MEDIUM; GENE FUNCTION; GENE LOCATION; GENETIC TRANSCRIPTION; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; PHENOTYPE; POINT MUTATION; PRIORITY JOURNAL; REGULATOR GENE; STRUCTURAL GENE;

EID: 0029805015     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/aac.40.12.2680     Document Type: Article

References (34)

1. Berger-Bachi, B., A. Strassle, and F. H. Kayser. 1986. Characterization of an isogenic set of methicillin-resistant and susceptible mutants of Staphylococcus aureus. Eur. J. Microbiol. 5:697-701.
2. Boyce, J. M., A. A. Medeiros, E. F. Papa, and C. J. O'Gara. 1990. Induction of beta-lactamase and methicillin resistance in unusual strains of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 25:73-81.
3. Camilli, A., D. A. Portnoy, and P. Youngman. 1990. Insertional mutagenesis of Listeria monocytogenes with a novel Tn917 derivative that allows direct cloning of DNA flanking transposon insertions. J. Bacteriol. 172:3738-3744.
4. Chang, S., and N. Cohen. 1979. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol. Gen. Genet. 168:111-115.
5. de Jonge, B. L. M., and A. Tomasz. 1993. Abnormal peptidoglycan produced in a methicillin-resistant strain of Staphylococcus aureus grown in the presence of methicillin: functional role for penicillin-binding protein 2A in cell wall synthesis. Antimicrob. Agents Chemother. 37:342-346.
6. Dyer, D. W., and J. J. Iandolo. 1983. Rapid isolation of DNA from Staphylococcus aurais. Appl. Environ. Microbiol. 46:283-285.
7. Hackbarth, C. J., T. Kocagoz, S. Kocagoz, and H. F. Chambers. 1995. Point mutations in Staphylococcus aureus PBP 2 gene affect penicillin-binding kinetics and are associated with resistance. Antimicrob. Agents Chemother. 39:103-106.
8. Hartman, B. J., and A. Tomasz. 1984. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus. J. Bacteriol. 158:513-516.
9. Hiramatsu, K. 1995. Molecular evolution of MRSA. Microbiol. Immunol. 39:531-543.
10. 9a. Hiramatsu, K. Unpublished data.
11. Hiramatsu, K., K. Asada, E. Suzuki, K. Okonogi, and T. Yokota. 1992. Molecular cloning and nucleotide sequence determination of the regulator region of mecA gene in methicillin-resistant Staphylococcus aureus (MRSA). FEBS Lett. 298:133-136.
12. Hiramatsu, K., K. Asada, E. Suzuki, and T. Yokota. 1994. Methicillin resistance regulation of the prototype MRSA strain N315. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. (Suppl.) 26:191-193.
13. Hiramatsu, K., H. Kihara, and T. Yokota. 1992. Analysis of borderline-resistant strains of methicillin-resistant Staphylococcus aureus using polymerase chain reaction. Microbiol. Immunol. 36:445-453.
14. Hurlimann-Dalei, R. L., C. Ryffel, F. H. Kayser, and B. Berger-Bachi. 1992. Survey of the methicillin-associated genes mecA, mecR1-mecI, and femA-femB in clinical isolates of methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 36:2617-2621.
15. Ishiwa, H., and H. Shibahara-Sone. 1986. New shuttle vectors for Escherichia coli and Bacillus subtilis. IV. The nucleotide sequence of pHY300PLK and some properties in relation to transformation. Jpn. J. Genet. 61:515-528.
16. Matsuhashi, M., M. D. Song, F. Ishino, M. Wachi, M. Doi, M. Inoue, K. Ubukata, N. Yamashita,. and M. Konno. 1986. Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus. J. Bacteriol. 167:975-980.
17. McDougal, L. K., and C. Thornsberry. 1986. The role of β-lactamase in staphylococcal resistance to penicillinase-resistant penicillins and cephalosporins. J. Clin. Microbiol. 23:832-839.
18. Murakami, K., K. Nomura, M. Doi, and T. Yoshida. 1987. Production of low-affinity penicillin-binding protein by low- and high-resistance groups of methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 31:1307-1311.
19. Murakami. K., and A. Tomasz. 1989. Involvement of multiple genetic determinants in high-level methicillin resistance in Staphylococcus aureus. J. Bacteriol. 171:874-879.
20. National Committee for Clinical Laboratory Standards. 1990. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 2nd ed. Approved standard. NCCLS document M7-A2. National Committee for Clinical Laboratory Standards, Villanova, Pa.
21. Okonogi, K., Y. Noji, M. Kondo, A. Imada, and T. Yokota. 1989. Emergence of methicillin-resistant clones from cephamycin-resistant Staphylococcus aureus. J. Antimicob. Chemother. 24:637-645.
22. Reynolds, P. E., and C. Fuller. 1986. Methicillin-resistant strains of Staphylococcus aureus; presence of identical additional penicillin-binding protein in all strains examined. FEMS Microbiol. Lett. 33:251-254.
23. Saito, M., K. Sekiguchi, R. Yajima, M. Hina, R. C. Doss, and H. Kanno. 1995. Immunological detection of penicillin-binding protein 2′ of methicillin-resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides. J. Clin. Microbiol. 33:2498-2500.
24. Sekiguchi, K., M. Saito, and R. Yajima. 1995. Detection of methicillin-resistant Staphylococcus aureus (MRSA) with antibodies against synthetic peptides derived from penicillin-binding protein 2′. Microbiol. Immunol. 39:545-550.
25. Song, M. D., M. Wachi, M. Doi, F. Ishino, and M. Matsuhashi. 1987. Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion. FEBS Lett. 221:167-171.
26. Suzuki, E., K. Hiramatsu, and T. Yokota. 1992. Survey of methicillin-resistant clinical strains of coagulase-negative staphylococci for mecA gene distribution. Antimicrob. Agents Chemother. 36:429-434.
27. Suzuki, E., K. Kuwahara-Arai, J. F. Richardson, and K. Hiramatsu. 1993. Distribution of mec regulator genes in methicillin-resistant Staphylococcus clinical strains. Antimicrob. Agents Chemother. 37:1219-1226.
28. Tanaka, T., K. Okuzumi, A. Iwamoto, and K. Hiramatsu. 1995. A retrospective study of methicillin-resistant Staphylococcus aureus clinical strains in Tokyo university hospital. J. Infect. Chemother. 1:40-49.
29. Tesch, W., C. Ryffel, A. Strassle, F. H. Kayser, and B. Berger-Bachi. 1990. Evidence of a novel staphylococcal mec-encoded element (mecR) controlling expression of penicillin-binding protein 2′. Antimicrob. Agents Chemother. 34:1703-1706.
30. Tomasz, A., H. B. Drugeon, H. M. de Lencastre, O. Jabes, L. McDougal, and J. Bille. 1989. New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity. Antimicrob. Agents Chemother. 33:1869-1874.
31. Tomasz, A., S. Nachman, and H. Leaf. 1991. Stable classes of phenotypic expression in methicillin-resistant clinical isolates of staphylococci. Antimicrob. Agents Chemothcr. 35:124-129.
32. Ubukata, K., N. Yamashita, and M. Konno. 1985. Occurrence of a beta-lactam-inducible penicillin-binding protein in methicillin-resistant staphylococci. Antimicrob. Agents Chemother. 27:851-857.
33. Utsui, Y., and T. Yokota. 1985. Role of an altered penicillin-binding protein in methicillin- and cephem-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 28:397-403.
34. Yoshida, R., K. Murakami, J. F. Richardson, and K. Hiramatsu. Raised penicillin-binding of PBPs 1 and 2 are correlated with high methicillin resistance in a mecA-negative Staphylococcus aureus clinical strain 61/5896. Submitted for publication.