Interaction of native and mutant MecI repressors with sequences that regulate mecA, the gene encoding penicillin binding protein 2a in methicillin-resistant staphylococci

Journal of Bacteriology

Volumn 180, Issue 8, 1998, Pages 2160-2166

  Sharma, Vijay K ^a,c   Hackbarth, Corinne J ^b,d   Dickinson, Tanja M ^a   Archer, Gordon L ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c NATIONAL ANIMAL DISEASE CENTER   (United States)

^d Versicor Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEASE; PALINDROMIC DNA; PENICILLIN BINDING PROTEIN; PENICILLIN BINDING PROTEIN 2A; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL MUTATION; CONTROLLED STUDY; DRUG BINDING; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN INTERACTION; REPRESSOR GENE; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION;

BACTERIA (MICROORGANISMS); METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NEGIBACTERIA; STAPHYLOCOCCI; STAPHYLOCOCCUS AUREUS;

EID: 0031948277     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.8.2160-2166.1998     Document Type: Article

References (26)

1. Archer, G. L., D. M. Niemeyer, J. A. Thanassi, and M. J. Pucci. 1994. Dissemination among staphylococci of DNA sequences associated with methicillin resistance. Antimicrob. Agents Chemother. 38:447-454.
2. m identifies a new region required for promoter function. Nucleic Acids Res. 24: 450-457.
3. Burz, D. S., D. Beckett, N. Benson, and G. K. Ackers. 1994. Self-assembly of bacteriophage λ cI represser: effects of single-site mutations. Biochemistry 33:8399-8405.
4. Burz, D. S., and G. K. Ackers. 1996. Cooperativity mutants of bacteriophage λ cI repressor: temperature dependence of self-assembly. Biochemistry 35: 3341-3350.
5. Chambers, H. F., B. Hartman, and A. Tomasz. 1985. Increased amounts of a novel penicillin-binding protein in methicillin-resistant Staphylococcus aureus exposed to nafcillin. J. Clin. Invest. 76:325-331.
6. Chodosh, L. A. 1987. Mobility shift DNA-binding assay using gel electrophoresis, p. 12.0.1-12.4.16. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. D. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology. Unit 12.2.1, Suppl. 13. Greene Publishing Associates and Wiley Interscience, New York, N.Y.
7. Chou, P. Y., and G. D. Fasman. 1974. Prediction of protein conformation. Biochemistry 13:222-245.
8. Gaisferd, C., and P. E. Reynolds. 1989. Methicillun resistance in Staphylococcus epidermidis. Relationship between the additional penicillin-binding protein and an attachment transpeptidase. Eur. J. Biochem. 185:211-218.
9. Gregory, P. D., R. A. Lewis, S. P. Curnock, and K. G. H. Dyke. 1997. Studies of the repressor (BlaI) of β-lactamase synthesis in Staphylococcus aureus. Mol. Microbiol. 24:1025-1037.
10. Hardt, K., B. Joris, A. Lepage, R. Brasseur, J. O. Lampen, J.-M. Frére, A. L. Fink, and J.-M. Ghuysen. 1997. The penicillin sensory transducer, BlaR, involved in the inducibility of β-lactamase synthesis in Bacillus licheniformis is embedded in the plasma membrane via a four-α-helix bundle. Mol. Microbiol. 23:935-944.
11. Harrison, S. C., and A. K. Aggarwal. 1990. DNA recognition by proteins with the helix-turn-helix motif. Annu. Rev. Biochem. 59:933-969.
12. Hattori, M., and Y. Sakaki. 1986. Dideoxy sequencing method using denatured plasmid templates. Anal. Biochem. 152:232-238.
13. Hiramatsu, K., K. Asada, E. Suzuki, K. Okonogi, and T. Yokota. 1992. Molecular cloning and nucleotide sequence of the regulator region of mecA gene in methicillin resistant Staphylococcus aureus. FEBS Lett. 298:133-136.
14. Hiramatsu, K. 1995. Molecular evolution of MRSA. Microbiol. Immunol. 39:531-543.
15. Joris, B., K. Hardt, and J.-M. Ghuysen. 1994. Induction of β-lactamase and low-affinity penicillin binding protein 2′ synthesis in gram-positive bacteria, p. 505-515. In J.-M. Ghuysen and R. Hakenbeck (ed.), Bacterial cell wall. Elsevier Science, New York, N.Y.
16. Kreiswirth, B. N., S. Lofdahl, M. J. Betley, M. O'Reilly, P. M. Schlievert, M. S. Bergdoll, and R. P. Novick. 1983. The toxic shock exotoxin structural gene is not detectably transmitted by prophage. Nature (London) 305:709-712.
17. Lee, C. Y., S. L. Buranen, and Z. Ye. 1991. Construction of a single-copy integration vector for Staphylococcus aureus. Gene 103:101-105.
18. Luchansky, T. B., P. M. Muriana, and T. R. Klaenhammer. 1988. Electro-transformation of gram-positive bacteria, p. 1-3. In Bio-Rad technical bulletin no. 1350. Bio-Rad Laboratories, Richmond, Calif.
19. Morton, T. M., D. M. Eaton, J. L. Johnston, and G. L. Archer. 1993. DNA sequence and units of transcription of the conjugative transfer gene complex (trs) of Staphylococcus aureus plasmid pG01. J. Bacteriol. 175:4436-4447.
20. Niemeyer, D. M., M. J. Pucci, J. A. Thanassi, V. K. Sharma, and G. L. Archer. 1996. Role of mecA transcriptional regulation in the phenotypic expression of methicillin resistance in Staphylococcus aureus. J. Bacteriol. 178:5464-5471.
21. Thompson, N. E., and P. A. Pattee. 1981. Genetic transformation in Staphylococcus aureus: demonstration of a competence-conferring factor of bacteriophage 80α lysates. J. Bacteriol. 148:294-300.
22. Townsend, D. E., N. Ashdown, J. Bolton, and W. B. Grubb. 1985. The use of cetyltrimethylammonium bromide for the rapid isolation from Staphylococcus aureus of relaxable and nonrelaxed plasmid DNA suitable for in vitro manipulation. Lett. Appl. Microbiol. 1:87-94.
23. Sharma, V. K., J. L. Johnston, T. M. Morton, and G. L. Archer. 1994. Transcriptional regulation by TrsN of conjugative transfer genes on staphylococcal plasmid pG01. J. Bacteriol. 176:3445-3454.
24. Wittman, V., and H. C. Wong. 1988. Regulation of the penicillinase genes of Bacillus licheniformis: interaction of the pen repressor with its operators. J. Bacteriol. 170:3206-3212.
25. Wittman, V., H. C. Lin, and H. C. Wong. 1993. Functional domains of the penicillinase repressor of Bacillus licheniformis. J. Bacteriol. 175:7383-7390.
26. Yanisch-Perron, C. J., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13 mp18 and pUC19 vectors. Gene 33:103-109.