Preparation of electro-competent E. coli using salt-free growth medium

BioTechniques

Volumn 20, Issue 1, 1996, Pages 42-44

  Sharma, Rakesh C ^a   Schimke, Robert T ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA; SODIUM CHLORIDE;

ARTICLE; BACTERIAL GROWTH; CULTURE MEDIUM; ELECTROPORATION; ESCHERICHIA COLI; GENETIC TRANSFORMATION; NONHUMAN; CULTURE TECHNIQUE; GROWTH, DEVELOPMENT AND AGING;

CELL CULTURE TECHNIQUES; CULTURE MEDIA; ELECTROPORATION; ESCHERICHIA COLI; SODIUM CHLORIDE;

EID: 0029665023     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96201bm08     Document Type: Article

References (10)

1. Bio-Rad E. coli Transformation Apparatus. 1994. Operating Instructions and Application Guide. Bio-Rad Laboratories, Richmond, CA.
2. Dover, W.J. 1990. Electroporation of bacteria: a general approach to genetic transformation. Genet. Eng. 12:275-295.
3. Dover, W.J., J.F. Miller and C.W. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16:6127-6145.
4. Maniatis, T., E.F. Fritsch and J. Sambrook. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
5. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
6. Sharma, R.C. and K.C. Smith. 1987. Role of DNA Polymerase I in postreplication repair: a reexamination with Escherichia coli Δ polA. J. Bacteriol. 769:4559-4564.
7. Sharma, R.C. and K.C. Smith. 1987. Comparison of the rep-38 and mmrA1 mutations of Escherichia coli. Mutat. Res. 184:23-28.
8. Sharma, R.C. and K.C. Smith. 1986. Repair of DNA double-strand breaks in UV-irradiated Escherichia coli uvrB recF cells is inhibited by rich growth medium. Mutat. Res. 166:23-28.
9. Sharma, R.C. and K.C. Smith. 1985. A mechanism of rich medium inhibition of the repair of daughter-strand gaps in the deoxyribonucleic acid of UV-irradiated Escherichia coli K-12 uvrA. Mutat. Res. 146:177-183.
10. Sharma, R.C. and K.C. Smith. 1985. A minor pathway of postreplication repair in Escherichia coli is independent of recB recC and recF genes. Mutat. Res. 146:169-176.