RNA interference effectively inhibits mRNA accumulation and protein expression of hepatitis C virus core and E2 genes in human cells

Bioscience, Biotechnology and Biochemistry

Volumn 70, Issue 9, 2006, Pages 2049-2055

  Liu, Min ^a   Ding, Hui ^a   Zhao, Ping ^a   Qin, Zhao Ling ^a   Gao, Jun ^a   Cao, Ming Mei ^a   Luan, Jie ^a   Wu, Wen Bin ^a   Qi, Zhong Tian ^a  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Core protein; Envelope protein; Hepatitis C virus; RNA; RNA interference; Small interfering

Indexed keywords

CORE PROTEIN; ENVELOPE PROTEIN; HEPATITIS C VIRUS; RNA INTERFERENCE; SMALL INTERFERING;

CELLS; DISEASE CONTROL; DRUG PRODUCTS; ENZYME INHIBITION; PROTEINS; RNA;

GENETIC ENGINEERING;

CORE PROTEIN; GREEN FLUORESCENT PROTEIN; NUCLEOCAPSID PROTEIN, HEPATITIS C VIRUS; SMALL INTERFERING RNA; VIRUS DNA; VIRUS ENVELOPE PROTEIN;

ARTICLE; BIOSYNTHESIS; CELL LINE; CHEMISTRY; DRUG ANTAGONISM; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GENETICS; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; METABOLISM; RNA INTERFERENCE; VIROLOGY; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL LINE; DNA, VIRAL; FLOW CYTOMETRY; GENE EXPRESSION REGULATION, VIRAL; GREEN FLUORESCENT PROTEINS; HEPACIVIRUS; HEPATITIS C, CHRONIC; HUMANS; MICROSCOPY, FLUORESCENCE; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSCRIPTION, GENETIC; TRANSFECTION; VIRAL CORE PROTEINS; VIRAL ENVELOPE PROTEINS;

HEPATITIS C VIRUS;

EID: 33748987349     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.60001     Document Type: Article

References (36)

1. Hannon, G. J., RNA interference. Nature, 418, 244-251 (2002).
2. Shi, Y., Mammalian RNAi for the masses. Trends Genet., 19, 9-12 (2003).
3. Dykxhoorn, D. M., Novina, C. D., and Sharp, P. A., Killing the messenger: short RNAs that silence gene expression. Nat. Rev. Mol. Cell. Biol., 4, 457-465 (2003).
4. Vance, V., and Vaucheret, H., RNA silencing in plants: defense and counterdefense. Science, 292, 2277-2280 (2001).
5. Cullen, B. R., RNA interference: antiviral defense and genetic tool. Nat. Immunol., 3, 597-599 (2002).
6. Clemens, J. C., Worby, C. A., Simonson-Leff, N., Muda, M., Maehama, T., Hemmings, B. A., and Dixon, J. E., Use of double-stranded RNA interference in Drosophila cell lines to dissect signal transduction pathways. Proc. Natl. Acad. Sci. USA, 97, 6499-6503 (2000).
7. McManus, M. T., and Sharp, P. A., Gene silencing in mammals by small interfering RNAs. Nat. Rev. Genet., 3, 737-747 (2002).
8. Bradley, D. W., Studies of non-A, non-B hepatitis and characterization of the hepatitis C virus in chimpanzees. Curr. Top. Microbiol. Immunol., 242, 1-23 (2000).
9. Alter, M. J., Margolis, H. S., Krawczynski, K., Judson, F. N., Mares, A., Alexander, W. J., Hu, P. Y., Miller, J. K., Gerber, M. A., and Sampliner, R. E., The natural history of community-acquired hepatitis C in the United States: the sentinel counties chronic non-A, non-B hepatitis study team. N. Engl. J. Med., 327, 1899-1905 (1992).
10. Diepolder, H. M., Jung, M. C., Keller, E., Schraut, W., Gerlach, J. T., Gruner, N., Zachoval, R., Hoffmann, R. M., Schirren, C. A., Scholz, S., and Pape, G. R., A vigorous virus-specific CD4+ T cell response may contribute to the association of HLA-DR13 with viral clearance in hepatitis B. Clin. Exp. Immunol., 113, 244-251 (1998).
11. Gruner, N. H., Gerlach, T. J., Jung, M. C., Diepolder, H. M., Schirren, C. A., Schraut, W. W., Hoffmann, R., Zachoval, R., Santantonio, T., Cucchiarini, M., Cerny, A., and Pape, G. R., Association of hepatitis C virus-specific CD8+ T cells with viral clearance in acute hepatitis C. J. Infect. Dis., 181, 1528-1536 (2000).
12. Chisari, F. V., Cytotoxic T cells and viral hepatitis. J. Clin. Invest., 99, 1472-1477 (1997).
13. Lanford, R. E., and Bigger, C., Advances in model systems for hepatitis C virus research. Virology, 293, 1-9 (2002).
14. Christie, J. M., and Chapman, R. W., Combination therapy for chronic hepatitis C: interferon and ribavirin. Hosp. Med., 60, 357-361 (1999).
15. McCaffrey, A. P., Ohashi, K., Meuse, L., Shen, S., Lancaster, A. M., Lukavsky, P. J., Sarnow, P., and Kay, M. A., Determinants of hepatitis C translational initiation in vitro, in cultured cells and mice. Mol. Ther., 5, 676-684 (2002).
16. Reed, K. E., and Rice, C. M., Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties. Curr. Top. Microbiol. Immunol., 242, 55-84 (2000).
17. Bartenschlager, R., and Lohmann, V., Replication of hepatitis C virus. Gen. Virol., 81, 1631-1648 (2000).
18. Penin, F., Dubuisson, J., Rey, F. A., Moradpour, D., and Pawlotsky, J. M., Structural biology of hepatitis C virus. Hepatology, 39, 5-19 (2004).
19. Pileri, P., Uematsu, Y., Campagnoli, S., Galli, G., Falugi, F., Petracca, R., Weiner, A. J., Houghton, M., Rosa, D., Grandi, G., and Abrignani, S., Binding of hepatitis C virus to CD81. Science, 282, 938-941 (1998).
20. Crotta, S., Stilla, A., Wack, A., D'Andrea, A., Nuti, S., D'Oro, U., Mosca, M., Filliponi, F., Brunetto, R. M., Bonino, F., Abrignani, S., and Valiante, N. M., Inhibition of natural killer cells through engagement of CD81 by the major hepatitis C virus envelope protein. J. Exp. Med., 195, 35-41 (2002).
21. Tseng, C. T., and Klimpel, G. R., Binding of the hepatitis C virus envelope protein E2 to CD81 inhibits natural killer cell functions. J. Exp. Med., 195, 43-49 (2002).
22. Machida, K., Cheng, K. T., Pavio, N., Sung, V. M., and Lai, M. M., Hepatitis C virus E2-CD81 interaction induces hypermutation of the immunoglobulin gene in B cells. J. Virol., 79, 8079-8089 (2005).
23. Zhao, L. J., Liu, H. Q., Cao, J., Feng, G. S., and Qi, Z. T., Activation of intracellular MAPK/ERK initiated by hepatitis C virus envelope protein E2 in HepG2 cells. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (in Chinese), 33, 691-695 (2001).
24. Marathe, R., Anandalakshmi, R., Smith, T. H., Pruss, G. J., and Vance, V. B., RNA viruses as inducers, suppressors and targets of post-transcriptional gene silencing. Plant Mol. Biol., 43, 295-306 (2000).
25. Hall, I. M., Shankaranarayana, G. D., Noma, K., Ayoub, N., Cohen, A., and Grewal, S. I., Establishment and maintenance of a heterochromatin domain. Science, 297, 2232-2237 (2002).
26. Wilson, J. A., Jayasena, S., Khvorova, A., Sabatinos, S., Rodrigue-Gervais, I. G., Arya, S., Sarangi, F., Harris-Brandts, M., Beaulieu, S., and Richardson, C. D., RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells. Proc. Natl. Acad. Sci. USA, 100, 2783-2788 (2003).
27. Cao, M. M., Ren, H., Pan, X., Pan, W., and Qi, Z. T., Inhibition of EGFP expression by siRNA in EGFP-stably expressing Huh-7 cells. J. Virol. Methods, 119, 189-194 (2004).
28. Rajeevan, M. S., Vernon, S. D., Taysavang, N., and Unger, E. R., Validation of array-based gene expression profiles by real-time (kinetic) RT-PCR. J. Mol. Diagn., 3, 26-31 (2001).
29. Winer, J., Jung, C. K., Shackel, I., and Williams, P. M., Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal. Biochem., 270, 41-49 (1999).
30. Novina, C. D., Murray, M. F., Dykxhoorn, D. M., Beresford, P. J., Riess, J., Lee, S. K., Collman, R. G., Lieberman, J., Shankar, P., and Sharp, P. A., siRNA-directed inhibition of HIV-1 infection. Nat. Med., 8, 681-686 (2002).
31. Coburn, G. A., and Cullen, B. R., Potent and specific inhibition of human immunodeficiency virus type 1 replication by RNA interference. J. Virol., 76, 9225-9231 (2002).
32. Lee, N. S., Dohjima, T., Bauer, G., Li, H., Li, M. J., Ehsani, A., Salvaterra, P., and Rossi, J., Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol., 20, 500-505 (2002).
33. Chang, J., Yang, S. H., Cho, Y. G., Hwang, S. B., Hahn, Y. S., and Sung, Y. C., Hepatitis C virus core from two different genotypes has an oncogenic potential but is not sufficient for transforming primary rat embryo fibroblasts in cooperation with the H-ras oncogene. J. Virol., 72, 3060-3065 (1998).
34. Ray, R. B., Lagging, L. M., Meyer, K., and Ray, R., Hepatitis C virus core protein cooperates with ras and transforms primary rat embryo fibroblasts to tumorigenic phenotype. J. Virol., 70, 4438-4443 (1996).
35. Moriya, K., Fujie, H., Shintani, Y., Yotsuyanagi, H., Tsutsumi, T., Ishibashi, K., Matsuura, Y., Kimura, S., Miyamura, T., and Koike, K., The core protein of hepatitis C virus induces hepatocellular carcinoma in transgenic mice. Nat. Med., 4, 1065-1067 (1998).
36. Giambartolomei, S., Covone, F., Levrero, M., and Balsano, C., Sustained activation of the Raf/MEK/Erk pathway in response to EGF in stable cell lines expressing the Hepatitis C Virus (HCV) core protein. Oncogene, 20, 2606-2610 (2001).