The catalytic machinery of chondroitinase ABC I utilizes a calcium coordination strategy to optimally process dermatan sulfate

Biochemistry

Volumn 45, Issue 37, 2006, Pages 11130-11139

  Prabhakar, Vikas ^a   Capila, Ishan ^a   Raman, Rahul ^a   Srinivasan, Aravind ^a   Bosques, Carlos J ^a   Pojasek, Kevin ^a   Wrick, Michael A ^a   Sasisekharan, Ram ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOASSAY; CALCIUM; CATALYSIS; COORDINATION REACTIONS; IONS; MUTAGENESIS;

CHONDROITIN SULFATE; CHONDROITINASE; DIVALENT IONS; KINETIC ASSAYS;

ENZYME KINETICS;

AMINO ACID; BACTERIAL ENZYME; CALCIUM; CALCIUM DERIVATIVE; CALCIUM ION; CHONDROITIN; CHONDROITIN ABC I LYASE; CHONDROITIN ABC LYASE; CHONDROITIN SULFATE; DERMATAN; DERMATAN SULFATE; DIVALENT CATION; FLUORESCENT DYE; GLYCOSAMINOGLYCAN; LYASE; TERBIUM; UNCLASSIFIED DRUG;

ARTICLE; BIOPHYSICS; CHEMICAL ANALYSIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; DEPOLYMERIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE ANALYSIS; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEUS VULGARIS; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

CALCIUM; CATALYSIS; CATIONS, DIVALENT; CHONDROITIN ABC LYASE; DERMATAN SULFATE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); PROTEUS VULGARIS;

EID: 33748759854     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0605484     Document Type: Article

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