Heparinase II from Flavobacterium heparinum: Role of cysteine in enzymatic activity as probed by chemical modification and site-directed mutagenesis

Journal of Biological Chemistry

Volumn 273, Issue 36, 1998, Pages 22904-22912

  Shriver, Zachary ^a   Hu, Yini ^a   Pojasek, Kevin ^b   Sasisekharant, Ram ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 CHLOROMERCURIBENZOIC ACID; 5,5' DITHIOBIS(2 NITROBENZOIC ACID); CYSTEINE; GLYCOSAMINOGLYCAN; HEPARAN SULFATE; HEPARIN; HEPARIN LYASE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MODIFICATION; ESCHERICHIA COLI; FLAVOBACTERIUM; FLAVOBACTERIUM HEPARINUM; GLYCOSAMINOGLYCAN METABOLISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; NONHUMAN; PH; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STOICHIOMETRY; STRUCTURE ACTIVITY RELATION;

BINDING SITES; CYSTEINE; FLAVOBACTERIUM; HEPARIN; HEPARIN LYASE; HEPARITIN SULFATE; HYDROGEN-ION CONCENTRATION; METALLOENDOPEPTIDASES; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; POLYSACCHARIDE-LYASES; RECOMBINANT PROTEINS; SULFHYDRYL REAGENTS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FLAVOBACTERIUM; NEGIBACTERIA; PEDOBACTER HEPARINUS;

EID: 0032483440     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.273.36.22904     Document Type: Article

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