The calcium-binding sites of heparinase I from Flavobacterium heparinum are essential for enzymatic activity

Journal of Biological Chemistry

Volumn 274, Issue 7, 1999, Pages 4089-4095

  Liu, Dongfang ^a   Shriver, Zachary ^a   Godavarti, Ranga ^c   Venkataraman, Ganesh ^a,b   Sasisekharan, Ram ^a,b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

^c PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINE; ASPARTIC ACID; CALCIUM; GLUTAMIC ACID; GLYCINE; GLYCOSAMINOGLYCAN; HEPARIN LYASE; THREONINE;

ARTICLE; CALCIUM BINDING; CATALYSIS; DEPOLYMERIZATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; FLAVOBACTERIUM; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITES; CALCIUM; CATALYTIC DOMAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FLAVOBACTERIUM; HEPARIN; HEPARIN LYASE; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP;

BACTERIA (MICROORGANISMS); FELIS CATUS; FLAVOBACTERIUM; NEGIBACTERIA; PEDOBACTER HEPARINUS;

EID: 0033548130     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.7.4089     Document Type: Article

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