Heparinase II from Flavobacterium heparinum: Role of histidine residues in enzymatic activity as probed by chemical modification and site-directed mutagenesis

Journal of Biological Chemistry

Volumn 273, Issue 17, 1998, Pages 10160-10167

  Shriver, Zachary ^a   Hu, Yini ^a   Sasisekharan, Ram ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIETHYL PYROCARBONATE; GLYCOSAMINOGLYCAN; HEPARIN LYASE; HISTIDINE; HYDROXYLAMINE; MUTANT PROTEIN;

ARTICLE; CATALYSIS; CHEMICAL MODIFICATION; CONCENTRATION RESPONSE; CONTROL SYSTEM; ENZYME ASSAY; ENZYME INACTIVATION; ENZYME MECHANISM; FLAVOBACTERIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

BINDING SITES; DIETHYL PYROCARBONATE; ENZYME ACTIVATION; FLAVOBACTERIUM; HEPARIN; HEPARIN LYASE; HEPARITIN SULFATE; HISTIDINE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MOLECULAR PROBES; MUTAGENESIS, SITE-DIRECTED;

EID: 0032562573     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.273.17.10160     Document Type: Article

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