Heparinase III from Flavobacterium heparinum: Cloning and recombinant expression in Escherichia coli

Biochemical and Biophysical Research Communications

Volumn 225, Issue 3, 1996, Pages 751-758

  Godavarti, Ranga ^a   Davis, Matthew ^a   Venkataraman, Ganesh ^a   Cooney, Charles ^a   Langer, Robert ^a   Sasisekharan, Ram ^b  

^a USA   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HEPARIN; HEPARIN LYASE; POLYSACCHARIDE; PROTEIN PRECURSOR; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; FLAVOBACTERIUM; GENE AMPLIFICATION; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 0030598887     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.1996.1246     Document Type: Article

References (32)

1. 1. Kjellèn, L., and Lindahl, U. (1991) Ann. Rev. Biochem. 60, 443-75.
2. 2. Rapraeger, A. C., Krufka, A., and Olwin, B. B. (1991) Science 252, 1705-08.
3. 3. Yayon, A., Klagsbrun, M., et al. (1991) Cell 64, 841-48.
4. 4. Ornitz, D. M., Yayon, A., et al. (1992) Mol. Cell. Biol. 12, 240-47.
5. 5. Gitay-Goren, H., Soker, S., Vlodavsky, I., and Neufeld, G. (1992) J. Biol. Chem. 267, 6093-98.
6. 6. Rapraeger, A. C. (1993) Curr. Op. Cell Biol. 5, 844-53.
7. 7. Ernst, S. E., Langer, R. S., Cooney, C. L., and Sasisekharan, R. (1995) Critical Reviews in Biochemistry and Molecular Biology 30, 387-444.
8. 8. Linker, A., and Hovingh, P. (1972) Meth. Enzymol. 28, 902-911.
9. 9. Silva, M. E., Dietrich, C. P., and Nader, H. B. (1976) Biochim. Biophys. Acta 437, 129-41.
10. 10. Ototani, N., Kikuchi, M., and Yosizawa, Z. (1981) Carb. Res. 88, 291-303.
11. 11. Linhardt, R. J., Fitzgerald, G. L., Cooney, C. L., and Langer, R. (1982) Biochim. Biophys. Acta 702, 197-203.
12. 12. Yang, V. C., Linhardt, R. J., et al. (1985) J. Biol. Chem. 260, 1849-57.
13. 13. Linhardt, R. J., Galliher, P. M., and Cooney, C. L. (1986) Appl. Biochem. Biotech. 12, 135-75.
14. 14. Linhardt, R. J., Turnbull, J. E., et al. (1990) Biochemistry 29, 2611-17.
15. 15. Lohse, D. L., and Linhardt, R. J. (1992) J. Biol. Chem. 267, 23347-55.
16. 16. Desai, U. R., Wang, H., and Linhardt, R. J. (1993) Biochemistry 32, 8140-45.
17. 17. Jandik, K. A., Gu, K., and Linhardt, R. J. (1994) Glycobiology 4, 289-96.
18. 18. Jin, L., Nakajima, M., and Nicolson, G. L. (1990) Int. J. Cancer 45, 1088-95.
19. 19. Graham, L. D. (1994) Biochem. J. 301, 917-918.
20. 20. Sasisekharan, R., Moses, M. A., et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1524-28.
21. 21. Sasisekharan, R., Bulmer, M., et al. (1993) Proc. Natl. Acad. Sci. USA 90, 3660-64.
22. 22. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. 23. Sasisekharan, R., Leckband, D., et al. (1995) Biochemistry 34, 14441-14448.
24. 24. Laemmli, U. K. (1970) Nature 227, 680-85.
25. 25. Godavarti, R. (1996) Ph.D. thesis. Massachusetts Institute of Technology, Cambridge.
26. 26. Sasisekharan, R., Venkataraman, G., et al. (1996) J. Biol. Chem. 271, 3124-3131.
27. 27. Ernst, E., Venkataraman, G., Winkler, S., Godavarti, R., Langer, R., Cooney, C., and Sasisekharan, R. (1996) Biochem. J. 315, 589-597.
28. 28. Godavarti, R., Cooney, C. L., Langer, R., and Sasisekaharan, R. (1996) Biochemistry 35, 6846-6852.
29. 29. Pickersgill, R., Jenkins, J., et al. (1994) Structural Biology 1, 717-23.
30. 30. Yoder, M. D., Keen, N. T., and Jurnak, F. (1993) Science 260, 1503-07.
31. 31. Yoder, M. D., Lietzke, S. E., and Jurnak, F. (1993) Structure 1, 241-51.
32. 32. Hoogewerf, A. J., Leone, J. W., Reardon, I. M., Howe, W. J., Asa, D., Heinrikson, R. L., and Ledbetter, S. R. (1995) J. Biol. Chem. 270, 3268-3277.