The expression of recombinant human LOX-1 and identifying its mimic ligands by fluorescence polarization-based high throughput screening

Journal of Biotechnology

Volumn 125, Issue 4, 2006, Pages 492-502

  Zhang, Tiantai ^a   Huang, Zhentai ^a   Dai, Ying ^a   Chen, Xiuping ^a   Zhu, Ping ^a   Du, Guanhua ^a  

^a INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

Atherosclerosis; Endothelial dysfunction; Fluorescence polarization; High throughput screening; Lectin like low density lipoprotein receptor 1

Indexed keywords

ATHEROSCLEROSIS; ENDOTHELIAL DYSFUNCTION; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING;

FLUORESCENCE; HUMAN ENGINEERING; OXIDATION; POLARIZATION;

PROTEINS;

COMPLEMENTARY DNA; DICHLORODIHYDROFLUORESCEIN DIACETATE; DIMETHYL SULFOXIDE; LIGAND; OXIDIZED LOW DENSITY LIPOPROTEIN; OXIDIZED LOW DENSITY LIPOPROTEIN RECEPTOR 1; REACTIVE OXYGEN METABOLITE; RECEPTOR PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; ATHEROGENESIS; ATHEROSCLEROSIS; CHO CELL; CONTROLLED STUDY; ENDOTHELIUM CELL; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; HUMAN; LIGAND BINDING; LIPID OXIDATION; NONHUMAN; PICHIA PASTORIS; PLASMID; PRIORITY JOURNAL; PROTEIN ANALYSIS; RECEPTOR BINDING; VASCULAR ENDOTHELIUM; WESTERN BLOTTING;

ANIMALS; BINDING, COMPETITIVE; CHO CELLS; CHOLESTEROL, LDL; CRICETINAE; DIMETHYL SULFOXIDE; DRUG STABILITY; FLUORESCENCE POLARIZATION; GENE EXPRESSION; HUMANS; LIGANDS; MOLECULAR MIMICRY; RECOMBINANT PROTEINS; SCAVENGER RECEPTORS, CLASS E; SUBSTRATE SPECIFICITY; TRANSFECTION;

PICHIA PASTORIS;

EID: 33748318206     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.03.040     Document Type: Article

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