Cloning and expression of human lectin-like oxidized low density lipoprotein receptor-1 in Pichia pastoris

Biotechnology Letters

Volumn 27, Issue 1, 2005, Pages 49-52

  Huang, Zhentai ^a   Zhang, Tiantai ^a   Yang, Jinling ^a   Zhu, Ping ^a   Du, Guanhua ^a   Cheng, Kedi ^a  

^a INSTITUTE OF MATERIA MEDICA   (China)

Author keywords

Heterologous expression; LOX 1; Pichia pastoris; Recombinant protein

Indexed keywords

BINDING ENERGY; BIOASSAY; CELLS; CHEMICAL MODIFICATION; CHROMATOGRAPHIC ANALYSIS; CLONING; OXIDATION; PURIFICATION;

BINDING ACTIVITY; GENE EXPRESSION; OXIDIZED LOW-DENSITY LIPOPROTEINS (OXLDL); RECOMBINANT PROTEINS;

PROTEINS;

LOW DENSITY LIPOPROTEIN RECEPTOR; OLR1 PROTEIN, HUMAN; OXIDIZED LOW DENSITY LIPOPROTEIN RECEPTOR; OXIDIZED LOW DENSITY LIPOPROTEIN RECEPTOR 1; PRIMER DNA; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; GENETICS; HUMAN; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PICHIA; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; TUMOR CELL LINE; WESTERN BLOTTING;

BASE SEQUENCE; BLOTTING, WESTERN; CELL LINE, TUMOR; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; PICHIA; PLASMIDS; RECEPTORS, LDL; RECEPTORS, OXIDIZED LDL; RECOMBINANT PROTEINS; SCAVENGER RECEPTORS, CLASS E;

PICHIA PASTORIS;

EID: 13844298641     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10529-004-6582-5     Document Type: Article

References (8)

1. Chen MY, Inoue K, Narumiya S, Masaki T, Sawamura T (2001) Requirements of basic amino acid residues within the lectin-like domain of LOX-1 for the binding of oxidized low-density lipoprotein. FEBS Lett. 499: 215-219.
2. Chen MY, Kakutani M, Minami M, Kataoka H, Kume N, Narumiya S, Kita T, Masaki T, Sawamura T (2000) Increased expression of lectin-like oxidized low density lipoprotein receptor-1 in initial atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits. Arterioscler. Thromb. Vasc. Biol. 20: 1107-1115.
3. Kataoka H, Kume N, Miyamoto S, Minami M, Murase T, Sawamura T, Masaki T, Hashimoto N, Kita T (2000) Biosynthesis and post-translational processing of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). N-linked glycosylation affects cell-surface expression and ligand binding. J. Biol. Chem. 275: 6573-6579.
4. Kume N, Kita T (2001) Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in atherosclerosis. Trends Cardiovasc. Med. 11: 22-25.
5. Masaki T (2003) Endothelial dysfunction and LOX-1. Circ. Res. 92: 819-820.
6. Murase T, Kume N, Kataoka H, Minami M, Sawamura T, Masaki T, Kita T (2000) Identification of soluble forms of lectin-like oxidized LDL receptor-1. Thromb. Vasc. Biol. 20: 715-720.
7. Sakurai K, Sawamura T (2003) Endothelial dysfunction via lectin-like oxidized lipoprotein receptor-1: close relationships with oxidative stress. J. Pharmacol. Sci. 91: 182-186.
8. Sawamura T, Kume N, Aoyama T, Moriwaki H, Hoshikawa H, Aiba Y, Tanaka T, Miwa S, Katsura Y, Kita T et al. (1997) An endothelial receptor for oxidized low-density lipoprotein. Nature 386: 73-77.