Enrichment and efficient screening of ES cells containing a targeted mutation: The use of DT-A gene with the polyadenylation signal as a negative selection maker

Transgenic Research

Volumn 8, Issue 3, 1999, Pages 215-221

  Yanagawa, Yuchio ^a,b   Kobayashi, Takashi ^a   Ohnishi, Motoko ^a   Kobayashi, Takayasu ^a   Tamura, Shinri ^a   Tsuzuki, Teruhisa ^c   Sanbo, Makoto ^b   Yagi, Takeshi ^b   Tashiro, Fumi ^a,d   Miyazaki, Jun ichi ^a,d  

^a TOHOKU UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES   (Japan)

^c KYUSHU UNIVERSITY   (Japan)

^d OSAKA UNIVERSITY MEDICAL SCHOOL   (Japan)

Author keywords

Diphtheria toxin; Gene targeting; LA PCR; Negative selection; Polyadenylation signal; Screening

Indexed keywords

DIPHTHERIA TOXIN A; GENE TARGETING; GENETIC SELECTION; HOMOLOGOUS RECOMBINATION; POLYADENYLATION; POLYMERASE CHAIN REACTION; STEM CELL; THYMIDINE KINASE;

ANIMALS; DIPHTHERIA TOXIN; EMBRYO; GENE TARGETING; GENETIC MARKERS; GLUTAMATE DECARBOXYLASE; MICE; POLY A; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; STEM CELLS; THYMIDINE KINASE;

EID: 0033150880     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008914020843     Document Type: Article

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