Targeting efficiency of α-1,3-galactosyl transferase gene in pig fetal fibroblast cells

Experimental and Molecular Medicine

Volumn 35, Issue 6, 2003, Pages 572-577

  Jin, Dong Il ^a,c   Lee, Seung Hyeon ^a,d   Choi, Jin Hee ^a   Lee, Jae Seon ^a   Lee, Jong Eun ^a   Park, Kwang Wook ^d   Seo, Jeong Sun ^a,b  

^a Seoul National University   (South Korea)

^b Seoul National University College of Medicine   (South Korea)

^c Chungnam National University   (South Korea)

^d MGenbio Inc   (South Korea)

Author keywords

Animal cloning; Galactosyltransferases; Gene targeting; Swine

Indexed keywords

ALPHA 1,3 GALACTOSYLTRANSFERASE; ANTIBIOTIC G 418; GALACTOSYLTRANSFERASE; GANCICLOVIR; GENOMIC DNA; HERPESVIRUS VECTOR; NEOMYCIN; THYMIDINE KINASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; DNA SCREENING; DRUG SELECTIVITY; EMBRYO CELL; FETUS; FIBROBLAST; GENE TARGETING; GENETIC RECOMBINATION; GENETIC TRANSFECTION; HERPES SIMPLEX VIRUS; KNOCKOUT GENE; NONHUMAN; SERUM; SOMATIC CELL GENETICS; STEM CELL; SWINE; TRANSGENIC ANIMAL; ANIMAL; GENE VECTOR; GENETICS; METABOLISM; POLYMERASE CHAIN REACTION; PRENATAL DEVELOPMENT;

ANIMALIA; HERPESVIRIDAE; SIMPLEXVIRUS; SUS SCROFA;

ANIMALS; FIBROBLASTS; GALACTOSYLTRANSFERASES; GENE TARGETING; GENETIC VECTORS; POLYMERASE CHAIN REACTION; SWINE;

EID: 1642541981     PISSN: 12263613     EISSN: 20926413     Source Type: Journal    
DOI: 10.1038/emm.2003.75     Document Type: Article

References (18)

1. Betthanser J, Forsberg E, Augenstein M, Childs L, Eilertsen K, Enos J, Forsythe T, Golueke P, Jurgella G, Koppang R, Lesmeister T, Mallon K, Mell G, Misica P, Pace M, Pfister-Geneskow M, Strelchenko N, Voelker G, Watt S, Thompson S, Bishop M. Production of cloned pigs from in vitro systems. Nature Biotech 2000;18:1055-9
2. Capecchi MR. Altering the genome by homologous recombination. Science 1989;244:1288-92
3. Cibelli JB, Stice, SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Leon AP, Robl JM. Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 1998;280:1256-8
4. Dai YD, Vaught T, Boone J, Chen SHJ, Phelps C, Ball SA, Monahan JM, Jobst PJ, McCreath KE, Lamborn AL, Cowell-Lucero JD, Wells K, Colman AA, Polejaeva IL, Ayares D. Targeted disruption of the α -1-,3galactosyltransferase gene in cloned pigs. Nature Biotech 2002;20:251-5
5. Denning C, Burl S, Ainslie A, Bracken J, Dinnyes A, Fletcher J, King T, Ritchie M, Ritchie WA, Rollo MP, de Sousa, Travers A, Wilmut I, Clark AJ. Deletion of theα (1,3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep. Nature Biotech 2001;19:559-62
6. Harrison SJ, Guidolin A, Faast R, Crocker LA, Giannakis C, D'Apice AJ, Nottle MB, Lyons I. Efficient generation of alpha (1,3) galactosyltransferase knockout porcine fetal fibroblasts for nuclear transfer. Transgenic Res 2002;11:143-50
7. Lai L, kolber-Simonds D, Park KW, Cheong HT, L.Greenstein J, Im GS, Samuel M, Bonk A, Rieke AN, Day BN, Murphy CB, Carter DJ, Hawley RS, Prather R. Production of α-1, 3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning. Science Express Report 2002;295:1089-9-92
8. McCreath KJ, Howcroft J, Campbell KHS, Colman A, Schnieke AE, Kind AJ. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 2000;405:1066-9
9. Mansour SL, Thomas KR, Capecchi MR. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature 1988;336:348-52
10. Onishi A, Iwamoto M, Akita T, Mikawa S, Takeda K, Awata T, Hanada H, Perry ACF. Pig cloning by microinjection of fetal fibroblast nuclei. Science 2000;289:1188-90
11. Phelps CJ, Koike C, Vaught TD, Boone J, Wells KD, Chen SH, Ball S, Specht SM, Polejaeva IA, Monahan JA, Jobst PM, Sharma SB, Lamborn AE, Garst AS, Moore M, Demetris AJ, Rudert WA, Bottino R, Bertera S, Trucco M, Starzl TE, Dai Y, Ayares DL. 2003. Production of alpha 1,3-galactosyltransferase-deficient pigs. Science 2002;299:411-4
12. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KHS. Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 2000;407:86-90
13. Ramsoondar JJ, Machaty Z, Costa C, Williams BL, Fodor WL, Bondioli KR. Production of alpha 1,3-galactosyltransferase-knockout cloned pigs expressing human alpha 1,2-fucosylosyltransferase. Biol Reprod 2003;69:437-45
14. Riele HT, Maandag ER, Berns A. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc Natl Acad Sci USA 1992;89:5128-32
15. Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A, Campbell KHS. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science 1997;278:2130-3
16. Snedecor GW, Cochran WG. 1967. Statistical Methods, The Iowa State University Press, Ames, IA, USA
17. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS. Viable offspring derived from fetal and adult mammalian cells Nature 1997;385:810-3
18. Zakhartchenko V, Mueller S, Alberio R, Schernthaner W, Stojkovic M, Wenigerkind H, Wanke R, Lassnig C, Mueller M, Wolf E, Brem G. Nuclear transfer in cattle with non-transfected and transfected fetal or cloned transgenic fetal and postnatal fibroblasts. Mol Reprod Dev 2001;60:362-9