Obtaining of ScFv-CBD fusion protein and its application for affinity purification of recombinant human interferon α2b

Ukrain'skyi Biokhimichnyi Zhurnal

Volumn 78, Issue 2, 2006, Pages 52-61

  Gilchuk, P V ^a   Okunev, O V ^b   Pavlova, M V ^a   Irodov, D M ^b   Gorbatyuk, O B ^a  

^a TARAS SHEVCHENKO NATIONAL UNIVERSITY OF KYIV   (Ukraine)

^b INSTITUTE OF MOLECULAR BIOLOGY AND GENETICS   (Ukraine)

Author keywords

Affinity purification; Bacterial inclusion bodies; Cellulose binding domain; Fusion proteins; Protein immobilization; Protein refolding; Single chain antibodies

Indexed keywords

ALPHA2B INTERFERON; CELLULOSE; HYBRID PROTEIN; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN FV; MONOCLONAL ANTIBODY;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL INCLUSION; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN RENATURATION;

ANTIBODIES, MONOCLONAL; CELLULOSE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; IMMUNOGLOBULIN FRAGMENTS; INCLUSION BODIES; INTERFERON ALFA-2B; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN RENATURATION; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI;

EID: 33746612927     PISSN: 02018470     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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