Search for Bacillus anthracis potential vaccine candidates by a functional genomic-serologic screen

Infection and Immunity

Volumn 74, Issue 7, 2006, Pages 3987-4001

  Gat, Orit ^a   Grosfeld, Haim ^a   Ariel, Naomi ^a   Inbar, Itzhak ^a   Zaide, Galia ^a   Broder, Yehoshua ^a   Zvi, Anat ^a   Chitlaru, Theodor ^a   Altboum, Zeev ^a   Stein, Dana ^a   Cohen, Sara ^a   Shafferman, Avigdor ^a  

^a ISRAEL INSTITUTE FOR BIOLOGICAL RESEARCH   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; ANTHRAX VACCINE; DNA VACCINE; MEMBRANE PROTEIN; OLIGOPEPTIDE; PEPTIDE TRANSPORTER 1;

ANTHRAX; ANTIBODY RESPONSE; ANTIGENICITY; ARTICLE; BACILLUS ANTHRACIS; FUNCTIONAL GENOMICS; GENETIC SCREENING; HUMORAL IMMUNITY; IMMUNE RESPONSE; IMMUNOPRECIPITATION; IMMUNOREACTIVITY; NONHUMAN; OPEN READING FRAME; PATHOGENESIS; PRIORITY JOURNAL; SEROLOGY; VACCINE PRODUCTION;

ANIMALS; ANTHRAX; ANTHRAX VACCINES; ANTIGENS, BACTERIAL; BACILLUS ANTHRACIS; CHROMOSOMES, BACTERIAL; COMPUTATIONAL BIOLOGY; GENOME, BACTERIAL; GENOMICS; GUINEA PIGS; IMMUNE SERA; MICE; MICE, INBRED ICR; OPEN READING FRAMES; RABBITS; VACCINES, DNA;

EID: 33745617720     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.00174-06     Document Type: Article

References (95)

1. Adu-Bobie, J., B. Capecchi, D. Serruto, R. Rappuoli, and M. Pizza. 2003. Two years into reverse vaccinology. Vaccine 21:605-610.
2. Aloni-Grinstein, R., O. Gat, Z. Altboum, B. Velan, S. Cohen, and A. Shafferman. 2005. Oral spore vaccine based on live attenuated nontoxinogenic Bacillus anthracis expressing recombinant mutant protective antigen. Infect. Immun. 73:4043-4053.
3. Altboum, Z., Y. Gozes, A. Barnea, A. Pass, M. White, and D. Kobiler. 2002. Postexposure prophylaxis against anthrax: evaluation of various treatment regimens in intranasally infected guinea pigs. Infect. Immun. 70:6231-6241.
4. Anantharaman, V., and L. Aravind. 2003. Evolutionary history, structural features and biochemical diversity of the NlpC/P60 superfamily of enzymes. Genome Biol. 4:R11.
5. 3+ siderophore transporter from Gram-positive pathogenic bacteria. Genome Biol. 3:5-9.
6. Andrade, M. A., C. Perez-Iratxeta, and C. P. Ponting. 2001. Protein repeats: structures, functions, and evolution. J. Struct. Biol. 134:117-131.
7. Antelmann, H., E. Darmon, D. Noone, J. W. Veening, H. Westers, S. Bron, O. P. Kuipers, K. M. Devine, M. Hecker, and J. M. van Dijl. 2003. The extracellular proteome of Bacillus subtilis under secretion stress conditions. Mol. Microbiol. 49:143-156.
8. Antelmann, H., H. Tjalsma, B. Voigt, S. Ohlmeier, S. Bron, J. M. van Dijl, and M. Hecker. 2001. A proteomic view on genome-based signal peptide predictions. Genome Res. 11:1484-1502.
9. Arciola, C. R., D. Campoccia, S. Gamberini, L. Baldassarri, and L. Montanaro. 2005. Prevalence of cna, fnbA and fnbB adhesin genes among Staphylococcus aureus isolates from orthopedic infections associated to different types of implant. FEMS Microbiol. Lett. 246:81-86.
10. Ariel, N., A. Zvi, H. Grosfeld, O. Gat, Y. Inbar, B. Velan, S. Cohen, and A. Shafferman. 2002. Search for potential vaccine candidate open reading frames in the Bacillus anthracis virulence plasmid pXO1: in silico and in vitro screening. Infect. Immun. 70:6817-6827.
11. Ariel, N., A. Zvi, K. S. Makarova, T. Chitlaru, E. Elhanany, B. Velan, S. Cohen, A. M. Friedlander, and A. Shafferman. 2003. Genome-based bioinformatic selection of chromosomal Bacillus anthracis putative vaccine candidates coupled with proteomic identification of surface-associated antigens. Infect. Immun. 71:4563-4579.
12. Baillie, L., and T. D. Read. 2001. Bacillus anthracis, a bug with attitude! Curr. Opin. Microbiol. 4:78-81.
13. Barnard, J. P., and A. M. Friedlander. 1999. Vaccination against anthrax with attenuated recombinant strains of Bacillus anthracis that produce protective antigen. Infect. Immun. 67:562-567.
14. Beecher, D. J., T. W. Olsen, E. B. Somers, and A. C. Wong. 2000. Evidence for contribution of tripartite hemolysin BL, phosphatidylcholine-preferring phospholipase C, and collagenase virulence of Bacillus cereus endophthalmitis. Infect. Immun. 68:5269-5276.
15. Bourgogne, A., M. Drysdale, S. G. Hilsenbeck, S. N. Peterson, and T. M. Koehler. 2003. Global effects of virulence gene regulators in a Bacillus anthracis strain with both virulence plasmids. Infect. Immun. 71:2736-2743.
16. Brossier, F., M. Levy, and M. Mock. 2002. Anthrax spores make an essential contribution to vaccine efficacy. Infect. Immun. 70:661-664.
17. Brown, J. S., and D. W. Holden. 2002. Iron acquisition by Gram-positive bacterial pathogens. Microbes Infect. 4:1149-1156.
18. Cabanes, D., P. Dehoux, O. Dussurget, L. Frangeul, and P. Cossart. 2002. Surface proteins and the pathogenic potential of Listeria monocytogenes. Trends Microbiol. 10:238-245.
19. Cabanes, D., O. Dussurget, P. Dehoux, and P. Cossart. 2004. Auto, a surface associated autolysin of Listeria monocytogenes required for entry into eukaryotic cells and virulence. Mol. Microbiol. 51:1601-1614.
20. Cendrowski, S., W. MacArthur, and P. Hanna. 2004. Bacillus anthracis requires siderophore biosynthesis for growth in macrophages and mouse virulence. Mol. Microbiol. 51:407-417.
21. Charlton, S., A. J. Moir, L. Baillie, and A. Moir. 1999. Characterization of the exosporium of Bacillus cereus. J. Appl. Microbiol. 87:241-245.
22. Chhatwal, G. S. 2002. Anchorless adhesins and invasins of Gram-positive bacteria: a new class of virulence factors. Trends Microbiol. 10:205-208.
23. Chitlaru, T., N. Ariel, A. Zvi, M. Lion, B. Velan, A. Shafferman, and E. Elhanany. 2004. Identification of chromosomally encoded membranal polypeptides of Bacillus anthracis by a proteomic analysis: prevalence of proteins containing S-layer homology domains. Proteomics 4:677-691.
24. 2-dependent cross-talk mechanisms modulate extracellular proteolytic activities. J. Bacteriol. 188:3551-3571.
25. Cohen, S., I. Mendelson, Z. Altboum, D. Kobiler, E. Elhanany, T. Bino, M. Leitner, I. Inbar, H. Rosenberg, Y. Gozes, R. Barak, M. Fisher, C. Kronman, B. Velan, and A. Shafferman. 2000. Attenuated nontoxinogenic and nonencapsulated recombinant Bacillus anthracis spore vaccines protect against anthrax. Infect. Immun. 68:4549-4558.
26. Davison, S., E. Couture-Tosi, T. Candela, M. Mock, and A. Fouet. 2005. Identification of the Bacillus anthracis (gamma) phage receptor. J. Bacteriol. 187:6742-6749.
27. De Groot, A. S., and R. Rappuoli. 2004. Genome-derived vaccines. Expert Rev. Vaccines 3:59-76.
28. Edlund, T., I. Siden, and H. G. Boman. 1976. Evidence for two immune inhibitors from Bacillus thuringiensis interfering with the humoral defense system of saturniid pupae. Infect. Immun. 14:934-941.
29. Fedhila, S., P. Nel, and D. Lereclus. 2002. The InhA2 metalloprotease of Bacillus thuringiensis strain 407 is required for pathogenicity in insects infected via the oral route. J. Bacteriol. 184:3296-3304.
30. Finco, O., A. Bonci, M. Agnusdei, M. Scarselli, R. Petracca, N. Norais, G. Ferrari, I. Garaguso, M. Donati, V. Sambri, R. Cevenini, G. Ratti, and G. Grandi. 2005. Identification of new potential vaccine candidates against Chlamydia pneumoniae by multiple screenings. Vaccine 23:1178-1188.
31. Fraser, C. M., and R. Rappuoli. 2005. Application of microbial genomic science to advanced therapeutics. Annu. Rev. Med. 56:459-474.
32. Friedlander, A. M., S. L. Welkos, and B. E. Ivins. 2002. Anthrax vaccines. Curr. Top. Microbiol. Immunol. 271:33-60.
33. Garmory, H. S., K. A. Brown, and R. W. Titball. 2002. Salmonella vaccines for use in humans: present and future perspectives. FEMS Microbiol. Rev. 26:339-353.
34. Gaspar, A. H., L. A. Marraffini, E. M. Glass, K. L. Debord, H. Ton-That, and O. Schneewind. 2005. Bacillus anthracis sortase A (SrtA) anchors LPXTG motif-containing surface proteins to the cell wall envelope. J. Bacteriol. 187:4646-4655.
35. Gat, O., H. Grosfeld, and A. Shafferman. In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation and immunoprecipitation analysis. In G. Grandi (ed.), Methods in molecular biology, in press. Humana Press, Totowa, N.J.
36. Gat, O., I. Inbar, R. Aloni-Grinstein, E. Zahavy, C. Kronman, I. Mendelson, S. Cohen, B. Velan, and A. Shafferman. 2003. Use of a promoter trap system in Bacillus anthracis and Bacillus subtilis for the development of recombinant protective antigen-based vaccines. Infect. Immun. 71:801-813.
37. Gat, O., I. Mendelson, T. Chitlaru, N. Ariel, Z. Altboum, H. Levy, S. Weiss, H. Grosfeld, S. Cohen, and A. Shafferman. 2005. The solute-binding component of a putative Mn(II) ABC transporter (MntA) is a novel Bacillus anthracis virulence determinant. Mol. Microbiol. 58:533-551.
38. Grosfeld, H., S. Cohen, T. Bino, Y. Flashner, R. Ber, E. Mamroud, C. Kronman, A. Shafferman, and B. Velan. 2003. Effective protective immunity to Yersinia pestis infection conferred by DNA vaccine coding for derivatives of the F1 capsular antigen. Infect. Immun. 71:374-383.
39. Heilmann, C., J. Hartleib, M. S. Hussain, and G. Peters. 2005. The multifunctional Staphylococcus aureus autolysin aaa mediates adherence to immobilized fibrinogen and fibronectin. Infect. Immun. 73:4793-4802.
40. Heusipp, G., K. M. Nelson, M. A. Schmidt, and V. L. Miller. 2004. Regulation of htrA expression in Yersinia enterocolitica. FEMS Microbiol. Lett. 231:227-235.
41. Ibrahim, Y. M., A. R. Kerr, J. McCluskey, and T. J. Mitchell. 2004. Role of HtrA in the virulence and competence of Streptococcus pneumoniae. Infect. Immun. 72:3584-3591.
42. Ivins, B. E., and S. L. Welkos. 1986. Cloning and expression of the Bacillus anthracis protective antigen gene in Bacillus subttlis. Infect. Immun. 54:537-542.
43. -) mutants of Bacillus anthracis and with recombinant strains of Bacillus subtilis that produce anthrax protective antigen. Infect. Immun. 58:303-308.
44. Jedrzejas, M. J. 2001. Pneumococcal virulence factors: structure and function. Microbiol. Mol. Biol. Rev. 65:187-207.
45. Kobiler, D., Y. Gozes, H. Rosenberg, D. Marcus, S. Reuveny, and Z. Altboum. 2002. Efficiency of protection of guinea pigs against infection with Bacillus anthracis spores by passive immunization. Infect. Immun. 70:544-560.
46. Kudva, I. T., R. W. Griffin, J. M. Garren, S. B. Calderwood, and M. John. 2005. Identification of a protein subset of the anthrax spore immunome in humans immunized with the anthrax vaccine adsorbed preparation. Infect. Immun. 73:5685-5696.
47. Lacy, D. B., and R. J. Collier. 2002. Structure and function of anthrax toxin. Curr. Top. Microbiol. Immunol. 271:61-85.
48. Lenz, L. L., S. Mohammadi, A. Geissler, and D. A. Portnoy. 2003. SecA2-dependent secretion of autolytic enzymes promotes Listeria monocytogenes pathogenesis. Proc. Natl. Acad. Sci. USA 100:12432-12437.
49. Leppla, S. H. 1995. Anthrax toxins, p. 543-572. In J. Moss, B. Iglewski, M. Vaughan, and A. T. Tu (ed.), Bacterial toxins and virulence factors in disease. Marcel Dekker, New York, N.Y.
50. Little, S. F., and G. B. Knudson. 1986. Comparative efficacy of Bacillus anthracis live spore vaccine and protective antigen vaccine against anthrax in the guinea pig. Infect. Immun. 52:509-512.
51. Loessner, M. J. 2005. Bacteriophage endolysins-current state of research and applications. Curr. Opin. Microbiol. 8:480-487.
52. Loosmore, S. M., Y. P. Yang, R. Oomen, J. M. Shortreed, D. C. Coleman, and M. H. Klein. 1998. The Haemophilus influenzae HtrA protein is a protective antigen. Infect. Immun. 66:899-906.
53. Lu, J. Z., T. Fujiwara, H. Komatsuzawa, M. Sugai, and J. Sakon. 2005. Cell wall targeting domain of glycylglycine endopeptidase discriminates peptidoglycan cross-bridge. J. Biol. Chem. 281:549-558.
54. Maione, D., I. Margarit, C. D. Rinaudo, V. Masignani, M. Mora, M. Scarselli, H. Tettelin, C. Brettoni, E. T. Iacobini, R. Rosini, N. D'Agostino, L. Miorin, S. Buccato, M. Mariani, G. Galli, R. Nogarotto, V. Nardi Dei, F. Vegni, C. Fraser, G. Mancuso, G. Teti, L. C. Madoff, L. C. Paoletti, R. Rappuoli, D. L. Kasper, J. L. Telford, and G. Grandi. 2005. Identification of a universal group B Streptococcus vaccine by multiple genome screen. Science 309:148-150.
55. Marino, M., L. Braun, P. Cossart, and P. Ghosh. 2000. A framework for interpreting the leucine-rich repeats of the Listeria internalins. Proc. Natl. Acad. Sci. USA 97:8784-8788.
56. Mazmanian, S. K., E. P. Skaar, A. H. Gaspar, M. Humayun, P. Gornicki, J. Jelenska, A. Joachmiak, D. M. Missiakas, and O. Schneewind. 2003. Passage of heme-iron across the envelope of Staphylococcus aureus. Science 299:906-909.
57. Mendelson, I., O. Gat, R. Aloni-Grinstein, Z. Altboum, I. Inbar, C. Kronman, E. Bar-Haim, S. Cohen, B. Velan, and A. Shafferman. 2005. Efficacious, nontoxigenic Bacillus anthracis spore vaccines based on strains expressing mutant variants of lethal toxin components. Vaccine 23:5688-5697.
58. Mesnage, S., T. Fontaine, T. Mignot, M. Delepierre, M. Mock, and A. Fouet. 2000. Bacterial SLH domain proteins are non-covalently anchored to the cell surface via a conserved mechanism involving wall polysaccharide pyruvylation. EMBO J. 19:4473-4484.
59. Mesnage, S., and A. Fouet 2002. Plasmid-encoded autolysin in Bacillus anthracis: modular structure and catalytic properties. J. Bacteriol. 184:331-334.
60. Mesnage, S., E. Tosi-Couture, M. Mock, P. Gounon, and A. Fouet. 1997. Molecular characterization of the Bacillus anthracis main S-layer component: evidence that it is the major cell-associated antigen. Mol. Microbiol. 23:1147-1155.
61. Mignot, T., M. Mock, and A. Fouet. 2003. A plasmid-encoded regulator couples the synthesis of toxins and surface structures in Bacillus anthracis. Mol. Microbiol. 47:917-927.
62. Mock, M., and A. Fouet 2001. Anthrax. Annu. Rev. Microbiol. 42:1189-1198.
63. Mock, M., and T. Mignot 2003. Anthrax toxins and the host: a story of intimacy. Cell. Microbiol. 5:15-23.
64. Montigiani, S., F. Falugi, M. Scarselli, O. Finco, R. Petracca, G. Galli, M. Mariani, R. Manetti, M. Agnusdei, R. Cevenini, M. Donati, R. Nogarotto, N. Norais, I. Garaguso, S. Nuti, G. Saletti, D. Rosa, G. Ratti, and G. Grandi. 2002. Genomic approach for analysis of surface proteins in Chlamydia pneumoniae. Infect. Immun. 70:368-379.
65. Moscoso, M., E. Lopez, E. Garcia, and R. Lopez. 2005. Implications of physiological studies based on genomic sequences: Streptococcus pneumoniae TIGR4 synthesizes a functional LytC lysozyme. J. Bacteriol. 187:6238-6241.
66. Navarre, W. W., and O. Schneewind. 1999. Surface proteins of gram-positive bacteria and mechanisms of their targeting to the cell wall envelope. Microbiol. Mol. Biol. Rev. 63:174-229.
67. Okinaka, R. T., K. Cloud, O. Hampton, A. R. Hoffmaster, K. K. Hill, P. Keim, T. M. Koehler, G. Lamke, S. Kumano, J. Mahillon, D. Manter, Y. Martinez, D. Ricke, R. Svensson, and P. J. Jackson. 1999. Sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes. J. Bacteriol. 181:6509-6515.
68. Pallen, M. J., R. R. Chaudhuri, and I. R. Henderson. 2003. Genomic analysis of secretion systems. Curr. Opin. Microbiol. 6:519-527.
69. Pannucci, J., R. T. Okinaka, E. Williams, R. Sabin, L. O. Ticknor, and C. R. Kuske. 2002b. DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria. BMC Genomics 3:34-41.
70. Pezard, C., M. Weber, J. C. Sirard, P. Berche, and M. Mock. 1995. Protective immunity induced by Bacillus anthracis toxin-deficient strains. Infect. Immun. 63:1369-1372.
71. Phillips, R. W., and R. M. Roop, 2nd. 2001. Brucella abortus HtrA functions as an authentic stress response protease but is not required for wild-type virulence in BALB/c mice. Infect. Immun. 69:5911-5913.
72. Pizza, M., V. Scarlato, V. Masignani, M. M. Giuliani, B. Arico, M. Comanducci, G. T. Jennings, L. Baldi, E. Bartolini, B. Capecchi, C. L. Galeotti, E. Luzzi, R. Manetti, E. Marchetti, M. Mora, S. Nuti, G. Ratti, L. Santini, S. Savino, M. Scarselli, E. Storni, P. Zuo, M. Broeker, E. Hundt, B. Knapp, E. Blair, T. Mason, H. Tettelin, D. W. Hood, A. C. Jeffries, N. J. Saunders, D. M. Granoff, J. C. Venter, E. R. Moxon, G. Grandi, and R. Rappuoli. 2000. Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing. Science 287:1816-1820.
73. Ramarao, N., and D. Lereclus. 2005. The InhA1 metalloprotease allows spores of the B. cereus group to escape macrophages. Cell. Microbiol. 7:1357-1364.
74. Ratledge, C., and L. G. Dover. 2000. Iron metabolism in pathogenic bacteria. Annu. Rev. Microbiol. 54:881-941.
75. Read, T. D., S. N. Peterson, N. Tourasse, L. W. Baillie, I. T. Paulsen, K. E. Nelson, H. Tettelin, D. E. Fouts, J. A. Eisen, S. R. Gill, E. K. Holtzapple, O. A. Okstad, E. Helgason, J. Rilstone, M. Wu, J. F. Kolonay, M. J. Beanan, R. J. Dodson, L. M. Brinkac, M. Gwinn, R. T. DeBoy, R. Madpu, S. C. Daugherty, A. S. Durkin, D. H. Haft, W. C. Nelson, J. D. Peterson, M. Pop, H. M. Khouri, D. Radune, J. L. Benton, Y. Mahamoud, L. Jiang, I. R. Hance, J. F. Weidman, K. J. Berry, R. D. Plaut, A. M. Wolf, K. L. Watkins, W. C. Nierman, A. Hazen, R. Cline, C. Redmond, J. E. Thwaite, O. White, S. L. Salzberg, B. Thomason, A. M. Friedlander, T. M. Koehler, P. C. Hanna, A. B. Kolsto, and C. M. Fraser. 2003. The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria. Nature 423:81-86.
76. Reid, S. D., A. G. Montgomery, J. M. Voyich, F. R. DeLeo, B. Lei, R. M. Ireland, N. M. Green, M. Liu, S. Lukomski, and J. M. Musser. 2003. Characterization of an extracellular virulence factor made by group A Streptococcus with homology to the Listeria monocytogenes internalin family of proteins. Infect. Immun. 71:7043-7052.
77. Rhie, G. E., M. H. Roehrl, M. Mourez, R. J. Collier, J. J. Mekalanos, and J. Y. Wang. 2003. A dually active anthrax vaccine that confers protection against both bacilli and toxins. Proc. Natl. Acad. Sci. USA 100:10925-10930.
78. Rosch, J. W., and M. G. Caparon. 2005. The ExPortal: an organelle dedicated to the biogenesis of secreted proteins in Streptococcus pyogenes. Mol. Microbiol. 58:959-968.
79. Ross, B. C., L. Czajkowski, D. Hocking, M. Margetts, E. Webb, L. Rothel, M. Patterson, C. Agius, S. Camuglia, E. Reynolds, T. Littlejohn, B. Gaeta, A. Ng, E. S. Kuczek, J. S. Mattick, D. Gearing, and I. G. Barr. 2001. Identification of vaccine candidate antigens from a genomic analysis of Porphyromonas gingivalis. Vaccine 19:4135-4142.
80. Schaible, U. E., and S. H. Kaufmann. 2004. Iron and microbial infection. Nat. Rev. Microbiol. 2:946-953.
81. Schneerson, R., J. Kubler-Kielb, T. Y. Liu, Z. D. Dai, S. H. Leppla, A. Yergey, P. Backlund, J. Shiloach, F. Majadly, and J. B. Robbins. 2003. Poly(gamma-D-glutamic acid) protein conjugates induce IgG antibodies in mice to the capsule of Bacillus anthracis: a potential addition to the anthrax vaccine. Proc. Natl. Acad. Sci. USA 100:8945-8950.
82. Siden, I., G. Dalhammar, B. Telander, H. G. Boman, and H. Somerville. 1979. Virulence factors in Bacillus thuringiensis: purification and properties of a protein inhibitor of immunity in insects. J. Gen. Microbiol. 114:45-52.
83. Sillanpaa, J., Y. Xu, S. R. Nallapareddy, B. E. Murray, and M. Hook. 2004. A family of putative MSCRAMMs from Enterococcus faecalis. Microbiology 150:2069-2078.
84. Skaar, E. P., M. Humayun, T. Bae, K. L. DeBord, and O. Schneewind. 2004. Iron-source preference of Staphylococcus aureus infections. Science 305:1626-1628.
85. Smith, T. J., S. A. Blackman, and S. J. Foster. 2000. Autolysins of Bacillus subtilis: multiple enzymes with multiple functions. Microbiology 146:249-262.
86. Steen, A., G. Buist, K. J. Leenhouts, M. El Khattabi, F. Grijpstra, A L. Zomer, G. Venema, O. P. Kuipers, and J. Kok. 2003. Cell wall attachment of a widely distributed peptidoglycan binding domain is hindered by cell wall constituents. J. Biol. Chem. 278:23874-23881.
87. Sutcliffe, I. C., and D. J. Harrington. 2002. Pattern searches for the identification of putative lipoprotein genes in Gram-positive bacterial genomes. Microbiology 148:2065-2077.
88. Sylvestre, P., E. Couture-Tosi, and M. Mock. 2002. A collagen-like surface glycoprotein is a structural component of the Bacillus anthracis exosporium. Mol. Microbiol. 45:169-178.
89. Ton-That, H., L. A. Marraffini, and O. Schneewind. 2004. Protein sorting to the cell wall envelope of Gram-positive bacteria. Biochim. Biophys. Acta 1694:269-278.
90. Weiss, S., D. Kobiler, H. Levy, H. Marcus, A. Pass, N. Rothschild, and Z. Altboum. 2006. Immunological correlates for protection against intranasal challenge of Bacillus anthracis spores conferred by a protective antigen-based vaccine in rabbits. Infect. Immun. 74:394-398.
91. Williams, R. C., M. L. Rees, M. F. Jacobs, Z. Pragai, J. E. Thwaite, L. W. Baillie, P. T. Emmerson, and C. R. Harwood. 2003. Production of Bacillus anthracis protective antigen is dependent on the extracellular chaperone, PrsA. J. Biol. Chem. 278:18056-18062.
92. Wizemann, T. M, J. H. Heinrichs, J. E. Adamou, A. L. Erwin, C. Kunsch, G. H. Choi, S. C. Barash, C. A. Rosen, H. R. Masure, E. Tuomanen, A. Gayle, Y. A. Brewah, W. Walsh, P. Barren, R. Lathigra, M. Hanson, S. Langermann, S. Johnson, and S. Koenig. 2001. Use of a whole genome approach to identify vaccine molecules affording protection against Streptococcus pneumoniae infection. Infect. Immun. 69:1593-1598.
93. Xu, Y., X. Liang, Y. Chen, T. M. Koehler, and M. Hook. 2004. Identification and biochemical characterization of two novel collagen binding MSCRAMMs of Bacillus anthracis. J. Biol. Chem. 279:51760-51768.
94. Xu, Y., J. M. Rivas, E. L. Brown, X. Liang, and M. Hook. 2004. Virulence potential of the staphylococcal adhesin CNA in experimental arthritis is determined by its affinity for collagen. J. Infect. Dis. 189:2323-2333.
95. Zink, S. D., and D. L. Burns. 2005. Importance of srtA and srtB for growth of Bacillus anthracis in macrophages. Infect. Immun. 73:5222-5228.