Global effects of virulence gene regulators in a Bacillus anthracis strain with both virulence plasmids

Infection and Immunity

Volumn 71, Issue 5, 2003, Pages 2736-2743

  Bourgogne, Agathe ^a   Drysdale, Melissa ^a   Hilsenbeck, Susan G ^b   Peterson, Scott N ^c,d   Koehler, Theresa M ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

^c J CRAIG VENTER INSTITUTE   (United States)

^d GEORGE WASHINGTON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRAX TOXIN; VIRULENCE FACTOR;

ACPA GENE; ARTICLE; ATXA GENE; BACILLUS ANTHRACIS; BACTERIAL CHROMOSOME; BACTERIAL MEMBRANE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM MUTANT; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE TARGETING; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN SECRETION; REGULATOR GENE; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; TOXIN SYNTHESIS;

BACILLUS ANTHRACIS; BACTERIAL CAPSULES; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REGULATOR; OPEN READING FRAMES; PLASMIDS; TRANS-ACTIVATORS; VIRULENCE;

EID: 0037406725     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.71.5.2736-2743.2003     Document Type: Article

References (45)

1. Agaisse, H., M. Gominet, O. A. Okstad, A. B. Kolsto, and D. Lereclus. 1999. PlcR is a pleiotropic regulator of extracellular virulence factor gene expression in Bacillus thuringiensis. Mol. Microbiol. 32:1043-1053.
2. Ash, C., J. A. Farrow, M. Dorsch, E. Stackebrandt, and M. D. Collins. 1991. Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16S rRNA. Int. J. Syst. Bacteriol. 41:343-346.
3. Brennan, R. G., and B. W. Matthews. 1989, The helix-turn-helix DNA binding motif. J. Biol. Chem. 264:1903-1906.
4. Caldwell, R., R. Sapolsky, W. Weyler, R. R. Maile, S. C. Causey, and E. Ferrari. 2001. Correlation between Bacillus subtilis scoC phenotype and gene expression determined using microarrays for transcriptome analysis. J. Bacteriol. 183:7329-7340.
5. Cataldi, A., E. Labruyere, and M. Mock. 1990. Construction and characterization of a protective antigen-deficient Bacillus anthracis strain. Mol. Microbiol. 4:1111-1117.
6. 2/bicarbonate, affects AtxA synthesis. Infect. Immun. 65:2576-2582.
7. Dai, Z., J.-C. Sirard, M. Mock, and T. M. Koehler. 1995. The atxA gene product activates transcription of the anthrax toxin genes and is essential for virulence. Mol. Microbiol. 16:1171-1181.
8. Fouet, A., and M. Mock. 1996. Differential influence of the two Bacillus anthracis plasmids on regulation of virulence gene expression, Infect. Immun. 64:4928-4932.
9. Green, B. D., L. Battisti, T. M. Koehler, and C. B. Thorne. 1985. Demonstration of a capsule plasmid in Bacillus anthracis. Infect. Immun. 49:291-297.
10. Guidi-Rontani, C., M. Weber-Levy, E. Labruyere, and M. Mock. 1999. Germination of Bacillus anthracis spores within alveolar macrophages. Mol. Microbiol. 31:9-17.
11. Guignot, J., M. Mock, and A. Fouet. 1997. AtxA activates the transcription of genes harbored by both Bacillus anthracis virulence plasmids. FEMS Microbiol. Lett. 147:203-207.
12. Helgason, E., O. A. Okstad, D. A. Caugant, H. A. Johansen, A. Fouet, M. Mock, I. Hegna, and A.-B. Kolsto. 2000. Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis - one species on the basis of genetic evidence. Appl. Environ. Microbiol. 66:2627-2630.
13. 2-enhanced non-toxin gene expression in Bacillus anthracis. Infect. Immun. 65:3091-3099.
14. Hoffmaster, A. R., and T. M. Koehler. 1999. Autogenous regulation of the Bacillus anthracis pag operon. J. Bacteriol. 181:4485-4492.
15. Ivins, B. E., J. W. Ezzell, Jr., J. Jemski, K. W. Hedlund, J. D. Ristroph, and S. H. Leppla. 1986. Immunization studies with attenuated strains of Bacillus anthracis. Infect. Immun. 52:454-458.
16. Koehler, T. M. 2002. Bacillus anthracis genetics and virulence gene regulation, p. 143-164. In T. M. Koehler (ed.), Anthrax, vol. 271. Springer-Verlag, Berlin, Germany.
17. 2 and a trans-acting element activate transcription from one of two promoters. J. Bacteriol. 176:586-595.
18. Koide, A., and J. A. Hoch. 1994. Identification of a second oligopeptide transport system in Bacillus subtilis and determination of its role in sporulation. Mol. Microbiol. 13:417-426.
19. Koide, A., M. Perego, and J. A. Hoch. 1999. ScoC regulates peptide transport and sporulation initiation in Bacillus subtilis. J. Bacteriol. 181:4114-4117.
20. Kunst, F., N. Ogasawara, I. Moszer, A. M. Albertini, G. Alloni, V. Azevedo, M. G. Bertero, P. Bessieres, A. Bolotin, S. Borchert, R. Borriss, L. Boursier, A. Brans, M. Braun, S. C. Brignell, S. Bron, S. Brouillet, C. V. Bruschi, B. Caldwell, V. Capuano, N. M. Carter, S. K. Choi, J. J. Codani, I. F. Connerton, A. Danchin, et al. 1997. The complete genome sequence of the grampositive bacterium Bacillus subtilis. Nature 390:249-256.
21. Leppla, S. H. 1995. Anthrax toxins, p. 543-572. In J. Moss, B. Iglewski, M. Vaughan, and A. T. Tu (ed.), Bacterial toxins and virulence factors in disease. Marcel Dekker, New York, N.Y.
22. Mahillon, J., R. Rezsohazy, B. Hallet, and J. Delcour. 1994. IS231 and other Bacillus thuringiensis transposable elements: a review. Genetica 93:13-26.
23. Makino, S., M. Watarai, H. I. Cheun, T. Shirahata, and I. Uchida. 2002. Effect of the lower molecular capsule released from the cell surface of Bacillus anthracis on the pathogenesis of anthrax. J. Infect. Dis. 186:227-233.
24. Makino, S.-I., I. Uchida, N. Terakado, C. Sasakawa, and M. Yoshikawa. 1989. Molecular characterization and protein analysis of the cap region, which is essential for encapsulation in Bacillus anthracis. J. Bacteriol. 171: 722-730.
25. Mesnage, S., T. Fontaine, T. Mignot, M. Delepierre, M. Mock, and A. Fouet. 2000. Bacterial SLH domain proteins are non-covalently anchored to the cell surface via a conserved mechanism involving wall polysaccharide pyruvylation. EMBO J. 19:4473-4484.
26. Mesnage, S., and A. Fouet. 2002. Plasmid-encoded autolysin in Bacillus anthracis: modular structure and catalytic properties. J. Bacteriol. 184:331-334.
27. Mignot, T., M. Mock, D. Robichon, A. Landier, D. Lereclus, and A. Fouet. 2001. The incompatibility between the PlcR- and AtxA-controlled regulons may have selected a nonsense mutation in Bacillus anthracis. Mol. Microbiol. 42:1189-1198.
28. Okinaka, R. T., K. Cloud, O. Hampton, A. Hoffmaster, K. K. Hill, P. Keim, T. M. Koehler, G. Lamke, S. Kumano, J. Mahillon, D. Manter, Y. Martinez, D. Ricke, R. Svensson, and P. J. Jackson. 1999. The sequence and organization of pXO1, the large Bacillus anthracis plasmid harboring the anthrax toxin genes. J. Bacteriol. 181:6509-6515.
29. Peltoniemi, K., E. Vesanto, and A. Palva. 2002. Genetic characterization of an oligopeptide transport system from Lactobacillus delbrueckii subsp. bulgaricus. Arch. Microbiol. 177:457-467.
30. Perego, M. 1997. A peptide export-import control circuit modulating bacterial development regulates protein phosphatases of the phosphorelay. Proc. Natl. Acad. Sci. USA 94:8612-8617.
31. Perez-Martin, J., F. Rojo, and V. de Lorenzo. 1994. Promoters responsive to DNA bending: a common theme in prokaryotic gene expression. Microbiol. Rev. 58:268-290.
32. Peterson, S., R. T. Cline, H. Tettelin, V. Sharov, and D. A. Morrison. 2000. Gene expression analysis of the Streptococcus pneumoniae competence regulons by use of DNA microarrays. J. Bacteriol. 182:6192-6202.
33. Pezard, C., P. Berche, and M. Mock. 1991. Contribution of individual toxin components to virulence of Bacillus anthracis. Infect. Immun. 59:3472-3477.
34. Read, T. D., S. N. Peterson, N. Tourasse, L. W. Baillie, I. T. Paulsen, K. E. Nelson, H. Tettelin, D. E. Fouts, J. A. Eisen, S. R. Gill, E. K. Holtzapple, O. A. Økstad, E. Helgason, J. Rilstone, M. Wu, J. F. Kolonay, M. J. Beanan, R. J. Dodson, L. M. Brinkac, M. Gwinn, R. T. DeBoy, R. Madpu, S. C. Daugherty, A. S. Durkin, D. H. Haft, W. C. Nelson, J. D. Peterson, M. Pop, H. M. Khouri, D. Radune, J. L. Benton, Y. Mahamoud, L. Jiang, I. R. Hance, J. F. Weidman, K. J. Berry, R. D. Plaut, A. M. Wolf, K. L. Watkins, W. C. Nierman, A. Hazen, R. Cline, C. Redmond, J. E. Thwaite, O. White, S. L. Salzberg, B. Thomason, A. M. Friedlander, T. M. Koehler, P. C. Hanna, A. B. Kolstø, and C. M. Fraser. The genome sequence of Bacillus anthracis Ames and comparison to closely-related bacteria. Nature, in press.
35. Saile, E., and T. M. Koehler. 2002. Control of anthrax toxin gene expression by the transition state regulator abrB. J. Bacteriol. 184:370-380.
36. Slamti, L., and D. Lereclus. 2002. A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group. EMBO J. 21:4550-4559.
37. Sterne, M. 1939. The use of anthrax vaccines prepared from avirulent (uncapsulated) variants of Bacillus anthracis. Onderstepoort J. Vet. Sci. Anim. Ind. 13:307-312.
38. Strauch, M. A. 1993. Abr, a transition state regulator, p. 757-764. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and other gram-positive bacteria. ASM Press, Washington, D.C.
39. Stukey, J., and G. M. Carman. 1997. Identification of a novel phosphatase sequence motif. Protein Sci. 6:469-472.
40. Thorne, C. B., and F. C. Belton. 1957. An agar-diffusion method for titrating Bacillus anthracis immunizing antigen and its application to a study of antigen production. J. Gen. Microbiol. 17:505-516.
41. Ticknor, L. O., A. B. Kolsto, K. K. Hill, P. Keim, M. T. Laker, M. Tonks, and P. J. Jackson. 2001. Fluorescent amplified fragment length polymorphism analysis of Norwegian Bacillus cereus and Bacillus thuringiensis soil isolates. Appl. Environ. Microbiol. 67:4863-4873.
42. Uchida, I., K. Hashimoto, and N. Terakado. 1986. Virulence and immunogenicity in experimental animals of Bacillus anthracis strains harbouring or lacking 110 MDa and 60 MDa plasmids. J. Gen. Microbiol. 132(Pt. 2):557-559.
43. Uchida, I., J. M. Hornung, C. B. Thorne, K. R. Klimpel, and S. H. Leppla. 1993. Cloning and characterization of a gene whose product is a transactivator of anthracis toxin synthesis. J. Bacteriol. 175:5329-5338.
44. Uchida, I., S. Makino, T. Sekizaki, and N. Terakado. 1997. Cross-talk to the genes for Bacillus anthracis capsule synthesis by atxA, the gene encoding the trans-activator of anthrax toxin synthesis. Mol. Microbiol. 23:1229-1240.
45. Vietri, N. J., R. Marrero, T. A. Hoover, and S. L. Welkos. 1995. Identification and characterization of a trans-activator involved in the regulation of encapsulation by Bacillus anthracis. Gene 152:1-9.