A proteomic view on genome-based signal peptide predictions

Genome Research

Volumn 11, Issue 9, 2001, Pages 1484-1502

  Antelmann, Haike ^a   Tjalsma, Harold ^b   Voigt, Birgit ^a   Ohlmeier, Steffen ^a   Bron, Sierd ^b   Van Dijl, Jan Maarten ^c   Hecker, Michael ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; CYTOPLASM PROTEIN; LIPOPROTEIN; MEMBRANE PROTEIN; PROTEOME; SECRETORY PROTEIN; SIGNAL PEPTIDASE I; SIGNAL PEPTIDE;

ANIMAL CELL; ARTICLE; BACILLUS SUBTILIS; CELL GROWTH; CELL MUTANT; CELL SECRETION; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME SPECIFICITY; ENZYME SUBSTRATE; EUBACTERIUM; EXTRACELLULAR SPACE; GENE MUTATION; GENE SEQUENCE; GRAM POSITIVE BACTERIUM; MASS SPECTROMETRY; NONHUMAN; PEPTIDE ANALYSIS; PREDICTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN MODIFICATION; SIGNAL TRANSDUCTION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

AMINO ACID SEQUENCE; ASPARTIC ENDOPEPTIDASES; BACILLUS SUBTILIS; BACTERIAL PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GENOME, BACTERIAL; LIPOPROTEINS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN SORTING SIGNALS; PROTEOME; SERINE ENDOPEPTIDASES;

EID: 0034821694     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.182801     Document Type: Article

References (58)

1. Phosphate starvation-inducible proteins of Bacillus subtilis: Proteomics and transcriptional analysis
2. Cell wall structure, synthesis and turnover
3. Subunit II of Bacillus subtilis cytochrome c oxidase is a lipoprotein
4. The role of autolysins during vegetative growth of Bacillus subtilis 168
5. The role of autolysins during vegetative growth of Bacillus subtilis 168
6. Improved silverstaining of plant proteins, RNA and DNA polyacrylamide gels
7. Different mechanisms for thermal inactivation of Bacillus subtilis signal peptidase mutants
8. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
9. Competition between Sec- and Tat-dependent protein translocation in Escherichia coli
10. A Bayesian system integrating expression data with sequence patterns for localizing proteins: Comprehensive application to the yeast genome
11. Genome-wide analysis relating expression level with protein subcellular localization
12. Phosphate-starvation-inducible proteins in Bacillus subtilis: A two-dimensional gel electrophoresis study
13. Molecular analysis of three major wall-associated proteins of Bacillus subtilis 168: Evidence for processing of the product of a gene encoding a 258 kDa precursor two-domain ligand-binding protein
14. Cloning, sequencing, and disruption of the Bacillus subtilis sigma 28 gene
15. Localization of Bacillus subtilis sacU(Hy) mutations to two linked genes with similarities to the conserved procaryotic family of two-component signalling systems
16. Proteome analysis of Bacillus subtilis extracellular proteins: A two-dimensional protein electrophoretic study
17. Type III protein secretion systems in bacterial pathogens of animals and plants
18. TatC is a specificity determinant for protein secretion via the twin-arginine translocation pathway
19. Comparative proteome analysis of Mycobacterium bovis BCG strains: Towards functional genomics of microbial pathogens
20. Two evolutionarily closely related ABC transporters mediate the uptake of choline for synthesis of the osmoprotectant glycine betaine in Bacillus subtilis
21. The PrsA lipoprotein is essential for protein secretion in Bacillus subtilis and sets a limit for high-level secretion
22. Lysis genes of the Bacillus subtilis defective prophage PBSX
23. Pleiotropic mutations affecting sporulation conditions and the syntheses of extracellular enzymes in Bacillus subtilis 168
24. Deduced polypeptides encoded by the Bacillus subtilis sacU locus share homology with two-component sensor-regulator systems
25. The complete genome sequence of the gram-positive bacterium Bacillus subtilis
26. High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigmaD gene and is affected by a sin (flaD) mutation
27. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to introcellulose
28. Identification and immunogenicity of group A Streptococcus culture supernatant proteins
29. Properties of the menaquinol oxidase (Qox) and of qox deletion mutants of Bacillus subtilis
30. Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg
31. Lipid modification of prelipoproteins is dispensable for growth but essential for efficient protein secretion in Bacillus subtilis: Characterization of the lgt gene
32. Lytic enzymes associated with defective prophages of Bacillus subtilis: Sequencing and characterization of the region comprising the N-acetylmuramoyl-L-alanine amidase gene of prophage PBSX
33. Simultaneous and rapid isolation of bacterial and eukaryotic DNA and RNA: A new approach for isolating DNA
34. The wprA gene of Bacillus subtilis 168, expressed during exponential growth, encodes a cell-wall-associated protease
35. Characterization of the sigD transcription unit of Bacillus subtilis
36. The Bacillus subtilis flagellin gene (hag) is transcribed by the sigma 28 form of RNA polymerase
37. Structure of the core and central channel of bacterial flagella
38. Consensus predictions of membrane protein topology
39. Identification of human myocardial proteins separated by two-dimensional electrophoresis using an effective sample preparation for mass spectrometry
40. Overall protein content and induced enzyme components of the periplasm of Bacillus subtilis
41. The N-acetylmuramoyl-L-alanine amidase encoded by the Bacillus subtilis 168 prophage SP-beta
42. Mapping and identification of Mycobacterium tuberculosis proteins by two-dimensional gel electrophoresis, microsequencing and immunodetection
43. Predicting the topology of eukaryotic membrane proteins
44. Influence of a cell-wall-associated protease on production of alpha-amylase by Bacillus subtilis
45. Bacillus subtilis contains four closely related type 1 signal peptidases with overlapping substrate specificities. Constitutive and temporally controlled expression of different sip genes
46. Functional analysis of the secretory precursor processing machinery of Bacillus subtilis: Identification of a eubacterial homolog of archaeal and eukaryotic signal peptidases
47. The role of lipoprotein processing by signal peptidase II in the Gram-positive eubacterium bacillus subtilis. Signal peptidase II is required for the efficient secretion of alpha-amylase, a non-lipoprotein
48. Signal peptide-dependent protein transport in Bacillus subtilis: A genome-based survey of the secretome
49. A vector for systematic gene inactivation in Bacillus subtilis
50. Non-functional expression of Escherichia coli signal peptidase I in Bacillus subtilis
51. Identification of the potential active site of the signal peptidase SipS of Bacillus subtilis. Structural and functional similarities with LexA-like proteases
52. Leaky lactococcus cultures that externalize enzymes and antigens independently of culture lysis and secretion and export pathways
53. Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis
54. A new ABC transporter mediating the detachment of lipid-modified proteins from membranes
55. Holins: Form and function in bacteriophage lysis
56. B