Analysis of proto-oncogene and heat-shock protein gene expression in human derived cell-lines exposed in vitro to an intermittent 1.9 GHz pulse-modulated radiofrequency field

International Journal of Radiation Biology

Volumn 82, Issue 5, 2006, Pages 347-354

  Chauhan, Vinita ^a   Mariampillai, Anusiyanthan ^a   Gajda, Greg ^a   Thansandote, Artnarong ^a   McNamee, James ^a  

^a HEALTH CANADA   (Canada)

Author keywords

Cell phone; Gene expression; Heat shock protein; Proto oncogene; Radiofrequency field

Indexed keywords

HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 27; HEAT SHOCK PROTEIN 70; HEAT SHOCK PROTEIN 70B; MESSENGER RNA; MYC PROTEIN; PROTEIN C FOS; PROTEIN C JUN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; EVALUATION RESEARCH; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; IN VITRO STUDY; PRIORITY JOURNAL; PROTO ONCOGENE; PULSE RATE; RADIATION ABSORPTION; RADIOFREQUENCY; THERMAL EXPOSURE;

CELL LINE; CELLULAR PHONE; DOSE-RESPONSE RELATIONSHIP, RADIATION; ENVIRONMENTAL EXPOSURE; GENE EXPRESSION; HEAT-SHOCK PROTEINS; HL-60 CELLS; HUMANS; MICROWAVES; OXIDATIVE STRESS; PROTO-ONCOGENE PROTEINS; RADIATION DOSAGE; RADIO WAVES;

EID: 33745314029     PISSN: 09553002     EISSN: 13623095     Source Type: Journal    
DOI: 10.1080/09553000600771549     Document Type: Article

References (29)

1. Adair ER, Black DR. 2003. Thermoregulatory responses to RF energy absorption. Bioelectromagnetics Supplement 6:S17-38.
2. Ahlbom A, Green A, Kheifets L, Savitz D, Swerdlow A. 2004. ICNIRP (International Commission for Non-Ionizing Radiation Protection) Standing Committee on Epidemiology. Epidemiology of health effects of radiofrequency exposure. Environmental Health Perspectives 112:1741-1754.
3. Challis LJ. 2005. Mechanisms for interaction between RF fields and biological tissue. Bioelectromagnetics Supplements 7:S98-S106.
4. Chauhan V, Mariampillai A, Bellier PV, Qutob SS, Gajda G, Lemay E, Thansandote A, McNamee JP. 2006. Gene expression analysis of a human lymphoblastoma cell line exposed in vitro to an intermittent 1.9 GHz pulse-modulated radiofrequency field. Radiation Research 165:424-429.
5. Czyz J, Guan K, Zeng Q, Nikolova T, Meister A, Schonborn F, Schuderer J, Kuster N, Wobus AM. 2004. High frequency electromagnetic fields (GSM signals) affect gene expression levels in tumor suppressor p53-deficient embryonic stem cells. Bioelectromagnetics 25:296-307.
6. Finnie JW. 2005. Expression of the immediate early gene, c-fos, in mouse brain after acute global system for mobile communication microwave exposure. Pathology 37:231-233.
7. Fritze K, Wiessner C, Kuster N, Sommer C, Gass P, Hermann DM, Kiessling M, Hossmann KA. 1997. Effect of global system for mobile communication microwave exposure on the genomic response of the rat brain. Neuroscience 81:627-639.
8. Gajda GB, McNamee JP, Thansandote A, Boonpanyarak S, Lemay E, Bellier PV. 2002. Cylindrical waveguide applicator for in vitro exposure of cell culture samples to 1.9-GHz radiofrequency fields. Bioelectromagnetics 23:592-598.
9. Goodman R, Blank M. 2002. Insights into electromagnetic interaction mechanisms. Journal of Cellular Physiology 192:16-22.
10. Goswami PC, Albee LD, Parsian AJ, Baty JD, Moros EJ, Pickard WF, Roti Roti JL, Hunt CR. 1999. Proto-oncogene mRNA levels and activities of multiple transcription factors in C3H 10T 1/2 murine embryonic fibroblasts exposed to 835.62 and 847.74 MHz cellular phone communication frequency radiation. Radiation Research 151:300-309.
11. Ivaschuk OI, Jones RA, Ishida-Jones T, Haggren W, Adey WR, Phillips JL. 1997. Exposure of nerve growth factor-treated PC12 rat pheochromocytoma cells to a modulated radiofrequency field at 836.55 MHz: effects on c-jun and c-fos expression. Bioelectromagnetics 18:223-229.
12. Kwee S, Raskmark P. 1998. Changes in cell proliferation due to environmental non-ionizing radiation: 2. Microwave radiation, Bioelectrochemistry and Bioenergetics 44:251-255.
13. Lantow M, Schuderer J, Hartwig C, Simko M. 2006. Free radical release and HSP70 expression in two human immune-relevant cell lines after exposure to 1800 MHz radiofrequency radiation. Radiation Research 165:88-94.
14. Laszlo A, Moros EG, Davidson T, Bradbury M, Straube W, Roti Roti J. 2005. The heat-shock factor is not activated in mammalian cells exposed to cellular phone frequency microwaves. Radiation Research 164:163-172.
15. Lee S, Johnson D, Dunbar K, Dong H, Ge X, Kim YC, Wing C, Jayathilaka N, Emmanuel N, Zhou CQ, Gerber HL, Tseng CC, Wang SM. 2005a. 2.45 GHz radiofrequency fields alter gene expression in cultured human cells. FEBS Letters 579:4829-4836.
16. Lee JS, Huang TQ, Lee JJ, Pack JK, Jang JJ, Seo JS. 2005b. Subchronic exposure of hsp70.1-deficient mice to radiofrequency radiation. International Journal of Radiation Biology 81:781-792.
17. Leszczynski D, Joenvaara S, Reivinen J, Kuokka R. 2002. Non-thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: Molecular mechanism for cancer- and blood-brain barrier-related effects. Differentiation 70:120-129.
18. Lim H, Cook MM, Barker AT, Coulton LA. 2005. Effect of 900 MHz electromagnetic fields on nonthermal induction of heat-shock proteins in human leukocytes. Radiation Research 163:45-52.
19. Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402-408.
20. Miyakoshi J, Takemasa K, Takashima Y, Ding GR, Hirose H, Koyama S. 2005. Effects of exposure to a 1950 MHz radio frequency field on expression of Hsp70 and Hsp27 in human glioma cells. Bioelectromagnetics 26:251-257.
21. Moulder JE, Foster KR, Erdreich LS, McNamee JP. 2005. Mobile phones, mobile phone base stations and cancer: A review. International Journal of Radiation Biology 81:189-203.
22. Nikolova T, Czyz J, Rolletschek A, Blyszczuk P, Fuchs J, Jovtchev G, Schuderer J, Kuster N, Wobus AM. 2005. Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells. FASEB Journal 19:1686-1688.
23. Pacini S, Ruggiero M, Sardi I, Aterini S, Gulisano F, Gulisano M. 2002. Exposure to global system for mobile communication (GSM) cellular phone radiofrequency alters gene expression, proliferation, and morphology of human skin fibroblasts. Oncology Research 13:19-24.
24. Qutob SS, Chauhan V, Bellier PV, Yauk C, Douglas G, Williams A, Berndt L, Gajda G, Thansondote A, McNamee JP. 2006. Microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to 1.9 GHz pulse modulated radiofrequency fields. Radiation Research 65:636-644.
25. Simko M, Hartwig C, Lantow M, Lupke M, Mattsson MO, Rahman Q, Rollwitz J. 2006. Hsp70 expression and free radical release after exposure to non-thermal radio-frequency electromagnetic fields and ultrafine particles in human Mono Mac 6 cells. Toxicology Letters 161:73-82.
26. Stagg RB, Hawel LH 3rd, Pastorian K, Cain C, Adey WR, Byus CV. 2001. Effect of immobilization and concurrent exposure to a pulse-modulated microwave field on core body temperature, plasma ACTH and corticosteroid, and brain ornithine decarboxylase, Fos and Jun mRNA. Radiation Research 155: 584-592.
27. Strauss GH. 1991. Non-random cell killing in cryopreservation: implications for performance of the battery of leukocyte tests (BLT), I. Toxic and immunotoxic effects. Mutation Research 252:1-15.
28. Tian F, Nakahara T, Wake K, Taki M, Miyakoshi J. 2002. Exposure to 2.45 GHz electromagnetic fields induces hsp70 at a high SAR of more than 20 W/kg but not at 5W/kg in human glioma MO54 cells. International Journal of Radiation Research 78:433-440.
29. Whitehead TD, Brownstein BH, Parry JJ, Thompson D, Cha BA, Moros EG, Rogers BE, Roti Roti JL. 2005. Expression of the Proto-oncogene Fos after exposure to radiofrequency radiation relevant to wireless communications. Radiation Research 164:420-430.